antifreeze proteins
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Author(s):  
Predrag Jevtić ◽  
K. Wade Elliott ◽  
Shelby E. Watkins ◽  
Jonathan A. Sreter ◽  
Katarina Jovic ◽  
...  

Cryoprotection is of interest in many fields of research, necessitating a greater understanding of different cryoprotective agents. Antifreeze proteins have been identified that have the ability to confer cryoprotection in certain organisms. Antifreeze proteins are an evolutionary adaptation that contributes to the freeze resistance of certain fish, insects, bacteria, and plants. These proteins adsorb to an ice crystal's surface and restrict its growth within a certain temperature range. We investigated the ability of an antifreeze protein from the desert beetle Anatolica polita, ApAFP752, to confer cryoprotection in the frog Xenopus laevis. X. laevis eggs and embryos microinjected with ApAFP752 exhibited reduced damage and increased survival after a freeze/thaw cycle in a concentration-dependent manner. We also demonstrate that ApAFP752 localizes to the plasma membrane in eggs and embryonic blastomeres and is not toxic for early development. These studies show the potential of an insect antifreeze protein to confer cryoprotection in amphibian eggs and embryos.


Author(s):  
Abirami Baskaran ◽  
Radhakrishnan Manikkam ◽  
Manigundan Kaari ◽  
Gopikrishnan Venugopal ◽  
Somasundaram Thirugnanasambandham ◽  
...  
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2022 ◽  
Vol 34 (2) ◽  
pp. 255
Author(s):  
L. Correia ◽  
G. Leal ◽  
B. Alves ◽  
R. Batista ◽  
P. Mermillod ◽  
...  

2021 ◽  
Author(s):  
Kartik Kamat ◽  
Pavithra M. Naullage ◽  
Valeria Molinero ◽  
Baron Peters

Cryobiology ◽  
2021 ◽  
Vol 103 ◽  
pp. 181
Author(s):  
Heather E. Tomalty ◽  
Virginia K. Walker ◽  
Peter L. Davies

2021 ◽  
Vol 176 ◽  
pp. 94-103
Author(s):  
Lucas F.L. Correia ◽  
Bruna R.C. Alves ◽  
Ribrio I.T.P. Batista ◽  
Pascal Mermillod ◽  
Joanna M.G. Souza-Fabjan

Author(s):  
Shaoli Cui ◽  
Weijia Zhang ◽  
Xueguang Shao ◽  
Wensheng Cai

Author(s):  
M. Saginova ◽  
Zh. Akishev ◽  
A. Sarsen ◽  
A. Kiribayeva ◽  
B. Khassenov

For survival in cold conditions, many organisms have developed unique adaptive mechanisms based on the synthesis of antifreeze proteins, peptides and glycoproteins that prevent ice formation at negative temperatures. These molecules tend to bind ice crystals and lower the freezing point of the solution without the formation of large crystals. Antifreeze proteins (AFP) were found in almost all types of living organisms, including insects, fungus, yeasts, bacteria and plants. The gene of antifreeze protein - glucan endo-1,3-beta-D-glucosidase (ScGlu-3) from Secale cereale was cloned into shuttle vector pPICZαA. The competent cells of yeast Pichia pastoris GS115 were transformed and the producer strain was obtained, which secreted of ScGlu-3 into the culture medium using 3% methanol as the only carbon source. It was found by western blotting that the maximum accumulation of ScGlu-3 in the culture occurs after 48 hours of fermentation on a medium with methanol. Established that rScGlu-3 precipitates at 50-65% of ammonium sulfate.


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