scholarly journals Measuring oxygen saturation in retinal and choroidal circulations in rats using visible light optical coherence tomography angiography

2015 ◽  
Vol 6 (8) ◽  
pp. 2840 ◽  
Author(s):  
Siyu Chen ◽  
Ji Yi ◽  
Hao F. Zhang
Keyword(s):  
Optical Coherence Tomography ◽  
Visible Light ◽  
Oxygen Saturation ◽  
Optical Coherence Tomography Angiography ◽  
Optical Coherence

scholarly journals Retinal capillary oximetry with visible light optical coherence tomography

2020 ◽  
Vol 117 (21) ◽  
pp. 11658-11666 ◽  
Author(s):  
Shaohua Pi ◽  
Tristan T. Hormel ◽  
Xiang Wei ◽  
William Cepurna ◽  
Bingjie Wang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Metabolic Rate ◽  
Visible Light ◽  
Oxygen Saturation ◽  
Vascular Diseases ◽  
Retinal Vessels ◽  
Optical Coherence ◽  
Retinal Circulation ◽  
Retinal Capillary ◽  
Vascular Plexuses

Assessing oxygen saturation (sO2) remains challenging but is nonetheless necessary for understanding retinal metabolism. We and others previously achieved oximetry on major retinal vessels and measured the total retinal oxygen metabolic rate in rats using visible-light optical coherence tomography. Here we extend oximetry measurements to capillaries and investigate all three retinal vascular plexuses by amplifying and extracting the spectroscopic signal from each capillary segment under the guidance of optical coherence tomography (OCT) angiography. Using this approach, we measured capillary sO2in the retinal circulation in rats, demonstrated reproducibility of the results, validated the measurements in superficial capillaries with known perfusion pathways, and determined sO2responses to hypoxia and hyperoxia in the different retinal capillary beds. OCT capillary oximetry has the potential to provide new insights into the retinal circulation in the normal eye as well as in retinal vascular diseases.

scholarly journals Visible light optical coherence tomography angiography (vis-OCTA) and local microvascular retinal oximetry in human retina

2020 ◽  
Author(s):  
Weiye Song ◽  
Wenjun Shao ◽  
Wei Yi ◽  
Rongrong Liu ◽  
Manishi Desai ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Visible Light ◽  
Optical Coherence Tomography Angiography ◽  
Spectral Power ◽  
Spectral Power Density ◽  
Optical Coherence ◽  
Imaging Protocol ◽  
Spectral Contrast ◽  
Accurate Localization ◽  
Acquisition Speed

AbstractWe report herein the first visible light optical coherence tomography angiography (vis-OCTA) for human retinal imaging. Compared to the existing vis-OCT systems, we devised a spectrometer with a narrower bandwidth to increase the spectral power density for OCTA imaging, while retaining the major spectral contrast in the blood. We achieved a 100 kHz A-line rate, the fastest acquisition speed reported so far for human retinal vis-OCT. We rigorously optimized the imaging protocol such that a single acquisition takes <6 seconds with a field of view (FOV) of 3×7.8 mm2. The angiography enables accurate localization of microvasculature down to the capillary level and thus enables oximetry at vessels < 100 μm in diameter. We demonstrated microvascular hemoglobin oxygen saturation (sO2) at the feeding and draining vessels at the perifoveal region. The longitudinal repeatability was assessed by <5% coefficient of variation (CV). The unique capabilities of our vis-OCTA system may allow studies on the role of microvascular oxygen in various retinal pathologies.

scholarly journals Spectral Contrast Optical Coherence Tomography Angiography Enables Single-Scan Vessel Imaging

2018 ◽  
Author(s):  
James A. Winkelmann ◽  
Aya Eid ◽  
Graham Spicer ◽  
Luay M. Almassalha ◽  
The-Quyen Nguyen ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Visible Light ◽  
Lymphatic Vessel ◽  
Optical Coherence Tomography Angiography ◽  
Optical Coherence ◽  
Spectral Signatures ◽  
Spectral Contrast ◽  
Endogenous Contrast ◽  
Tissue Discrimination ◽  
Vessel Imaging

AbstractOptical coherence tomography angiography relies on motion for contrast and requires at least two data acquisitions per pointwise scanning location. We present a method termed spectral contrast optical coherence tomography angiography using visible light that relies on the spectral signatures of blood for angiography from a single scan using endogenous contrast. We demonstrate the molecular sensitivity of this method, which enables lymphatic vessel, blood, and tissue discrimination.

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