Highly selective behaviour of colon adenoma after administration of EMR composition and its HCT116-based model.

When applying the improved composition of the solution used during endoscopic mucosal resection (EMR), we observed unexpectedly large and quantitatively significant differences in adenoma response vs. healthy tissue of the surrounding GIT tract, namely, the selective reaction enhancing the adenoma volume and differentiated colour. The in vitro experiments on the model neoplasia cell line HCT116 suggest that the robust differences in the response of starving cells can be traced down principally to tetrastarch digestion and the enhanced metabolic rate of neoplastic cells. The neoplastic tissue grows into several intestine layers so that submucosal injection of iso-oncotic tetrastarch compound leads to degradation of starch and production of oncotic molecules in submucosa transported by facilitated transport into the neoplastic tissue. The colour distinction is due to concentration differences of the reporting dye between three separated compartments, further enhancing the utility of the contrasting mixture. The diffusion dynamics shall be tuneable by optimizing starch composition, improving desirable pharmacokinetics.

Clothing Insulation Rate and Metabolic Rate Estimation for Individual Thermal Comfort Assessment in Real Life

Satisfactory indoor thermal environments can improve working efficiencies of office staff. To build such satisfactory indoor microclimates, individual thermal comfort assessment is important, for which personal clothing insulation rate (Icl) and metabolic rate (M) need to be estimated dynamically. Therefore, this paper proposes a vision-based method. Specifically, a human tracking-by-detection framework is implemented to acquire each person’s clothing status (short-sleeved, long-sleeved), key posture (sitting, standing), and bounding box information simultaneously. The clothing status together with a key body points detector locate the person’s skin region and clothes region, allowing the measurement of skin temperature (Ts) and clothes temperature (Tc), and realizing the calculation of Icl from Ts and Tc. The key posture and the bounding box change across time can category the person’s activity intensity into a corresponding level, from which the M value is estimated. Moreover, we have collected a multi-person thermal dataset to evaluate the method. The tracking-by-detection framework achieves a mAP50 (Mean Average Precision) rate of 89.1% and a MOTA (Multiple Object Tracking Accuracy) rate of 99.5%. The Icl estimation module gets an accuracy of 96.2% in locating skin and clothes. The M estimation module obtains a classification rate of 95.6% in categorizing activity level. All of these prove the usefulness of the proposed method in a multi-person scenario of real-life applications.

Efficiency of treatment of laryngopharyngeal reflux with proton pump inhibitors depending on the CYP2C19 polymorphism

Introduction. A treatment for LFR for many years, the superiority of PPIs over placebos is still controversial. Of particular clinical importance is the metabolic rate of PPIs in hepatocytes using the cytochrome P450 system with the participation of the isoenzyme CYP2C19 and partially CYP3A4Аim. We set a goal to study the efficacy of omeprazole 20 mg in the treatment of LFR symptoms without esophageal syndrome in patients with gastroesophageal reflux (GERD), depending on the polymorphism of the CYP2C19 genotype.Мaterials and мethods. After the exclusion criteria, 100 people took part in the study, 94 people completed the study.Results. According to the results, 26.6% of patients in the study group (residents of the Moscow region) with LFR symptoms without esophageal syndrome belong to fast metabolizers of CYP2C19, 4.2% to ultrafast metabolizers, 52.1% to normal metabolizers, 16% to intermediate metabolizers and 1.1% to slow CYP2C19.Conclusions. In patients with a rapid metabolism, within 1 month after discontinuation of omeprazole, it is necessary to increase the amount of omeprazole 20 mg intake up to 2 times a day in the morning and in the evening and reduce the duration of treatment to 6 weeks.

Effects of a Single Dose of BURN-XT™ on Resting Metabolic Rate, Substrate Oxidation, and Various Indices of Affect

Introduction: Many consumers use dietary supplements in the hopes of increasing energy and burning more calories, which if sustained over time may help accelerate weight loss. The purpose of this clinical trial was to investigate the effects of an over-the-counter thermogenic supplement called Burn-XT™ (BXT) on metabolic rate, substrate oxidation, and various psychometric indices of affect that impact weight management. Methods: Using a double-blind, placebo-controlled, cross-over design, 16 women and 10 men (29.3 ± 7.3 yr, 169.4 ± 8.6 cm, 75.5 ± 14.3 kg) underwent two testing sessions: placebo (PL) and BXT. Seated metabolic rate and substrate oxidation, vital signs, and anchored visual analogue scale (VAS) assessments of energy, mood, motivation, focus, fatigue, concentration, and appetite were made before supplementation and hourly for three hours post-ingestion. Two-factor (2x4) factorial ANOVAs and paired sample t-tests (corrected for multiple comparisons) were used for analyses. Results: Significant increases in metabolic rate (oxygen consumption) were noted at 60 minutes in BXT (+11.9 mL O2/min) vs. PL (-2.5 mL O2/min), p = 0.004, d = -0.74. Only BXT increased metabolic rate compared to baseline at 60 minutes (+11.9 mL O2/min, p = 0.021, d = -0.53) and 120 minutes (+12.1 mL O2/min, p = 0.019, d = -0.54). The AUC for resting energy expenditure increased more in BXT vs. PL (p = 0.007, d = -0.57). VAS detected significant improvements in energy, mood, focus, and concentration for BXT vs. PL at 120 and 180 minutes (all p < 0.05, d = -0.58 to -0.68). In all cases, within-group changes from baseline for these VAS parameters were significant (all p < 0.05, d = -0.76 to -1.38) in BXT but not in PL. No within or between group differences in appetite, substrate oxidation, or heart rate were noted. Small (~3-4 mm Hg), but statistically significant (p < 0.05, d = -0.51 to -0.69) increases in diastolic blood pressure were noted in BXT at 60, 120, and 180 min vs. PL; and in systolic blood pressure at 60 min vs. PL. In all cases, values remained within normal clinical hemodynamic ranges. Conclusions: A single dose of BXT safely increased metabolic rate, energy, mood, focus, and concentration. Given that these factors are known to favorably impact weight management, future studies should determine whether daily supplementation with BXT reduces body weight and improves body composition.

Highly selective behaviour of colon adenoma after administration of EMR composition and its HCT116-based model.

When applying the improved composition of the solution used during endoscopic mucosal resection (EMR), we observed unexpectedly large and quantitatively significant differences in adenoma response vs. healthy tissue of the surrounding GIT tract, namely, the selective reaction enhancing the adenoma volume and differentiated colour. The in vitro experiments on the model neoplasia cell line HCT116 suggest that the robust differences in the response of starving cells can be traced down principally to tetrastarch digestion and the enhanced metabolic rate of neoplastic cells. The neoplastic tissue grows into several intestine layers so that submucosal injection of iso-oncotic tetrastarch compound leads to degradation of starch and production of oncotic molecules in submucosa transported by facilitated transport into the neoplastic tissue. The colour distinction is due to concentration differences of the reporting dye between three separated compartments, further enhancing the utility of the contrasting mixture. The diffusion dynamics shall be tuneable by optimizing starch composition, improving desirable pharmacokinetics.

Association Between Basal Metabolic Rate and All-Cause Mortality in a Prospective Cohort of Southern Chinese Adults

Objective: The aim of this study was to assess the relationship between basal metabolic rate (BMR) and all-cause mortality in southern Chinese adults.Methods: We prospectively examined the relationship between BMR and all-cause mortality in 12,608 Southern Chinese adults with age ≥ 35 years who participated in the National Key R&amp;D Program from 2013–2014 to 2019–2020. Cox proportional hazard models were used to examine the association between BMR and all-cause mortality.Results: A total of 809 deaths (including 478 men and 331 women) occurred during a median follow-up period of 5.60 years. All-cause mortality was higher in elderly individuals than in non-elderly individuals (11.48 vs. 2.04%, P &lt; 0.001) and was higher in male subjects than in female subjects (9.84 vs. 4.56%, P &lt; 0.001). There was a significantly inverse relationship between BMR levels and all-cause mortality in elderly male individuals (adjusted-HR per SD increase: 0.80, 95% CI: 0.70–0.91, P &lt; 0.001). Compared with BMR levels ≤ 1,115 kJ/day, there was lower all-cause mortality in third and highest BMR quartiles in the elderly male subjects (adjusted-HR: 0.71, 95% CI: 0.53–0.95, P = 0.022; adjusted-HR: 0.60, 95% CI: 0.43–0.84, P = 0.003, respectively).Conclusion: An elevated BMR was independently inversely associated with all-cause mortality in elderly male subjects in a southern Chinese population.

Behavioural microclimate selection and physiological responses to environmental conditions in a hibernating bat

Hibernators adjust the expression of torpor behaviourally and physiologically to balance the benefits of energy conservation in hibernation against the physiological and ecological costs. Small fat-storing species, like many cave-hibernating bats, have long been thought to be highly constrained in their expression of hibernation because they must survive winter relying only on endogenous energy stores. We evaluated behavioural microclimate selection in tri-colored bats (Perimyotis subflavus (Cuvier, 1832)) across a three-month hibernation experiment under laboratory conditions. We also opportunistically tested for evidence of acclimatization in torpid metabolic rate (TMR). When given access to gradients in microclimate, bats tended to choose the warmest temperature available (11°C) while almost completely avoiding the driest condition available (85% relative humidity at 8°C). Further, bats held at different temperatures over the course of the hibernation showed no differences in TMR when measured under common conditions at the end of hibernation. Taken together, our results suggest selective pressures to conserve energy during hibernation are not overwhelmingly strong and further support the proposition that optimal expression of hibernation is something less than the maximal expression of hibernation unless the animal is nearing starvation.

Influence of fat-free mass and resting metabolic rate on increased food reinforcement after exercise training

Purpose Models of appetite control have been largely based on negative feedback from gut and adipose signaling to central appetite centers. However, contemporary models posit that fat-free mass (FFM) or the energy demand of FFM [i.e., resting metabolic rate (RMR)] may play a primary role in the motivational drive for food intake (i.e., food reinforcement). The relative reinforcing value of food (RRVfood) is associated with energy intake (EI) and increases with an acute energy deficit. Chronic exercise-induced energy deficits lead to alterations in fat mass (FM), FFM, and RMR and provide an opportunity to test whether change in (∆) FM, ∆FFM, ∆usual EI, or ∆RMR are associated with ∆RRVfood. Methods Participants (n = 29, BMI = 25–35 kg/m2) engaged in aerobic exercise expending 300 or 600 kcal, 5 days/weeks for 12 weeks. The reinforcing value of food (PMaxfood) was measured via a computer-based operant responding task and RRVfood was calculated as the reinforcing value of food relative to non-eating sedentary behaviors. RMR was determined by indirect calorimetry and body composition by DXA. Results Post-training FFM correlated with usual post-training EI (rs = 0.41, p < 0.05), PMaxfood (rs=0.52, p < 0.01), and RMR (rs = 0.85, p < 0.0001). ∆RMR negatively correlated with ∆PMaxfood (rs = − 0.38, p < 0.05) and with ∆RRVfood (rs = − 0.37, p < 0.05). ∆PMaxfood and ∆RRVfood were not associated with ∆FFM (p = 0.71, p = 0.57, respectively). Conclusions Reductions in RMR with weight loss may increase food reinforcement as means of restoring FFM and RMR to pre-weight loss amounts. Limiting reductions in RMR during weight loss may benefit weight maintenance by restricting increases in food reinforcement after weight loss.