Selective excitation of fluorescent proteins on the basis of the two-photon absorption spectrum measurement

2006 ◽  
Author(s):  
M. Tanaka ◽  
J. Tada ◽  
F. Kannari ◽  
H. Kawano ◽  
H. Mizuno ◽  
...  
Keyword(s):  
Absorption Spectrum ◽  
Fluorescent Proteins ◽  
Selective Excitation ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Spectrum Measurement

Intensity ratios of ^2D_5/2,3/2 ← ^2S doublets in the two-photon absorption spectrum of Rb and Cs

1984 ◽  
Vol 9 (3) ◽  
pp. 82 ◽  
Author(s):  
F. Arqueros ◽  
P. E. LaRocque ◽  
M. S. O’Sullivan ◽  
B. P. Stoicheff
Keyword(s):  
Absorption Spectrum ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Intensity Ratios

Two-photon absorption spectrum of CdS in theA- andB-excitonic region at 20 °K by the new twin-laser technique

1974 ◽  
Vol 10 (8) ◽  
pp. 3471-3479 ◽  
Author(s):  
R. G. Stafford ◽  
M. Sondergeld
Keyword(s):  
Absorption Spectrum ◽  
Photon Absorption ◽  
Laser Technique ◽  
Two Photon Absorption ◽  
Two Photon

Two-photon absorption spectrum of ZnS

1968 ◽  
Vol 28 (4) ◽  
pp. 305-306 ◽  
Author(s):  
K. Park ◽  
H.S. Waff
Keyword(s):  
Absorption Spectrum ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon

Doppler-free two-photon absorption spectrum of rubidium

1979 ◽  
Vol 57 (12) ◽  
pp. 2143-2154 ◽  
Author(s):  
B. P. Stoicheff ◽  
E. Weinberger
Keyword(s):  
Absorption Spectrum ◽  
Hyperfine Structure ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Quantum Defects ◽  
Ionization Limit

Doppler-free, two-photon spectroscopy has been used to investigate the transitions n2S ← 52S and n2D ← 52S of atomic rubidium, from n = 9 to 116 and n = 7 to 124, respectively, only 7.3 cm−1 from the ionization limit. Wavelengths of these transitions have been measured to an accuracy of 1 part in 107, and series formulae computed for term values and quantum defects. Hyperfine structure splittings in the 92S to 132S levels have also been measured.

scholarly journals Multiphoton Bleaching of Red Fluorescent Proteins and the Ways to Reduce It

2022 ◽  
Vol 23 (2) ◽  
pp. 770
Author(s):  
Mikhail Drobizhev ◽  
Rosana S. Molina ◽  
Jacob Franklin
Keyword(s):  
Chemical Structure ◽  
Fluorescent Proteins ◽  
Multiphoton Ionization ◽  
Excitation Power ◽  
Photon Absorption ◽  
Green Fluorescent Proteins ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Red Fluorescent Proteins ◽  
Green Fluorescent

Red fluorescent proteins and biosensors built upon them are potentially beneficial for two-photon laser microscopy (TPLM) because they can image deeper layers of tissue, compared to green fluorescent proteins. However, some publications report on their very fast photobleaching, especially upon excitation at 750–800 nm. Here we study the multiphoton bleaching properties of mCherry, mPlum, tdTomato, and jREX-GECO1, measuring power dependences of photobleaching rates K at different excitation wavelengths across the whole two-photon absorption spectrum. Although all these proteins contain the chromophore with the same chemical structure, the mechanisms of their multiphoton bleaching are different. The number of photons required to initiate a photochemical reaction varies, depending on wavelength and power, from 2 (all four proteins) to 3 (jREX-GECO1) to 4 (mCherry, mPlum, tdTomato), and even up to 8 (tdTomato). We found that at sufficiently low excitation power P, the rate K often follows a quadratic power dependence, that turns into higher order dependence (K~Pα with α > 2) when the power surpasses a particular threshold P*. An optimum intensity for TPLM is close to the P*, because it provides the highest signal-to-background ratio and any further reduction of laser intensity would not improve the fluorescence/bleaching rate ratio. Additionally, one should avoid using wavelengths shorter than a particular threshold to avoid fast bleaching due to multiphoton ionization.

scholarly journals Two-Photon Absorption Properties of Chromophores of a Few Fluorescent Proteins: a Theoretical Investigation

2016 ◽  
Vol 32 (1) ◽  
pp. 301-312
Author(s):  
Chuan-Xiang YE ◽  
◽  
Hui-Li MA ◽  
Wan-Zhen LIANG ◽  
◽  
...  
Keyword(s):  
Theoretical Investigation ◽  
Fluorescent Proteins ◽  
Photon Absorption ◽  
Absorption Properties ◽  
Two Photon Absorption ◽  
Two Photon

Two photon absorption spectrum ofp-dichlorobenzene

Pramana ◽  
1995 ◽  
Vol 45 (3) ◽  
pp. 271-278
Author(s):  
I B Singh ◽  
S B Rai ◽  
D K Rai
Keyword(s):  
Absorption Spectrum ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon

scholarly journals Absolute Two-Photon Absorption Spectra and Two-Photon Brightness of Orange and Red Fluorescent Proteins

2009 ◽  
Vol 113 (4) ◽  
pp. 855-859 ◽  
Author(s):  
M. Drobizhev ◽  
S. Tillo ◽  
N. S. Makarov ◽  
T. E. Hughes ◽  
A. Rebane
Keyword(s):  
Absorption Spectra ◽  
Fluorescent Proteins ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Red Fluorescent Proteins
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