Practical aspects of super-resolution optical fluctuation image scanning microscopy (SOFISM)

2020 ◽  
Author(s):  
Adrian Makowski ◽  
Gur Lubin ◽  
Ron Tenne ◽  
Aleksandra Sroda ◽  
Uri Rossman ◽  
...  
Keyword(s):  
Super Resolution ◽  
Scanning Microscopy ◽  
Image Scanning

scholarly journals A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM

2019 ◽  
Vol 16 (2) ◽  
pp. 175-178 ◽  
Author(s):  
Marco Castello ◽  
Giorgio Tortarolo ◽  
Mauro Buttafava ◽  
Takahiro Deguchi ◽  
Federica Villa ◽  
...  
Keyword(s):  
Super Resolution ◽  
Scanning Microscopy ◽  
Image Scanning

scholarly journals Super-resolution microscopy based on parallel detection

2019 ◽  
Vol 12 (06) ◽  
pp. 1950023
Author(s):  
Zhimin Zhang ◽  
Shaocong Liu ◽  
Liang Xu ◽  
Yubing Han ◽  
Cuifang Kuang ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Signal To Noise Ratio ◽  
3D Structure ◽  
Super Resolution ◽  
Confocal Imaging ◽  
Scanning Microscopy ◽  
Resolution Limit ◽  
Image Scanning ◽  
Parallel Detection ◽  
Super Resolution Microscopy

Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C. J. R. Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988). C. B. Müller, E. Jörg, “Image scanning microscopy, “Phys. Rev. Lett. 104(19) 198101 (2010). C. J. R. Sheppard, S. B. Mehta, R. Heintzmann, “Superresolution by image scanning microscopy using pixel reassignment,” Opt. Lett. 38(15) 2889–2892 (2013)]. Here, we use a tailor-made optical fiber and 19 avalanche photodiodes (APDs) as parallel detectors to upgrade our existing confocal microscopy, termed as parallel-detection super-resolution (PDSR) microscopy. In order to obtain the correct shift value, we use the normalized 2D cross correlation to calculate the shifting value of each image. We characterized our system performance by imaging fluorescence beads and applied this system to observing the 3D structure of biological specimen.

scholarly journals Super-resolution enhancement by quantum image scanning microscopy

2018 ◽  
Vol 13 (2) ◽  
pp. 116-122 ◽  
Author(s):  
Ron Tenne ◽  
Uri Rossman ◽  
Batel Rephael ◽  
Yonatan Israel ◽  
Alexander Krupinski-Ptaszek ◽  
...  
Keyword(s):  
Resolution Enhancement ◽  
Super Resolution ◽  
Scanning Microscopy ◽  
Quantum Image ◽  
Image Scanning

scholarly journals SOFISM: Super-resolution optical fluctuation image scanning microscopy

Optica ◽  
2020 ◽  
Vol 7 (10) ◽  
pp. 1308 ◽  
Author(s):  
Aleksandra Sroda ◽  
Adrian Makowski ◽  
Ron Tenne ◽  
Uri Rossman ◽  
Gur Lubin ◽  
...  
Keyword(s):  
Super Resolution ◽  
Scanning Microscopy ◽  
Image Scanning

scholarly journals Structured illumination microscopy and image scanning microscopy: a review and comparison of imaging properties

2021 ◽  
Vol 379 (2199) ◽  
Author(s):  
Colin J. R. Sheppard
Keyword(s):  
Spatial Resolution ◽  
Super Resolution ◽  
Scanning Microscopy ◽  
Structured Illumination ◽  
Practical Application ◽  
Structured Illumination Microscopy ◽  
Image Scanning ◽  
Imaging Performance ◽  
Imaging Properties

Structured illumination microscopy and image scanning microscopy are two microscopical tech- niques, rapidly increasing in practical application, that can result in improvement in transverse spatial resolution, and/or improvement in axial imaging performance. The history and principles of these techniques are reviewed, and the imaging properties of the two methods compared. This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 1)’.

scholarly journals The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy

2021 ◽  
Vol 11 (19) ◽  
pp. 8981
Author(s):  
Colin J. R. Sheppard
Keyword(s):  
Confocal Microscopy ◽  
Biological Samples ◽  
Super Resolution ◽  
Scanning Microscopy ◽  
Optical Methods ◽  
Nonlinear Microscopy ◽  
Structured Illumination ◽  
Basic Principles ◽  
Image Scanning ◽  
Super Resolution Microscopy

Optical methods of super-resolution microscopy, such as confocal microscopy, structured illumination, nonlinear microscopy, and image scanning microscopy are reviewed. These methods avoid strong invasive interaction with a sample, allowing the observation of delicate biological samples. The meaning of resolution and the basic principles and different approaches to superresolution are discussed.

Super-resolution optical fluctuation image scanning microscopy (SOFISM)

2019 ◽  
Author(s):  
Aleksandra Sroda ◽  
Adrian Makowski ◽  
Uri Rossman ◽  
Gur Lubin ◽  
Ron Tenne ◽  
...  
Keyword(s):  
Super Resolution ◽  
Scanning Microscopy ◽  
Image Scanning

scholarly journals Super-resolution in confocal scanning microscopy

1987 ◽  
Vol 3 (2) ◽  
pp. 195-212 ◽  
Author(s):  
M Bertero ◽  
P Brianzi ◽  
E R Pike
Keyword(s):  
Super Resolution ◽  
Scanning Microscopy ◽  
Confocal Scanning ◽  
Confocal Scanning Microscopy
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