Serological and hematological characteristics of Sjogren’s syndrome and dry eye syndrome patients using a novel immune serology technique

Objectives To compare hematologic and serological parameters among patients with Sjogren’s syndrome (SS), dry eye syndrome (DES) and controls, and validate a novel multiplex-serology method for identifying auto-antibodies in these populations. Methods In a clinic-based case-control study a total of 422 participants were recruited, including 91 with SS, 120 DES, and 211 controls (age and sex frequency-matched). We measured blood counts, anti-nuclear-antibodies (ANA), anti-SSA/SSB, anti-ribonucleoprotein (RNP), anti-double-stranded-DNA (DS-DNA), and rheumatoid factor (RF) using the “Immunodot” qualitative-ELISA assay. Immunoglobulins, C3 and C4 were measured by immune-fluorescence. Autoantibodies were also quantified with a newly-developed method using glutathione-S-transferase fusion proteins of SSA/Ro 52 and 60kD and SSB/La (multiplex-serology), measuring median fluorescence intensity (MFI). Results Among DES patients, only 2% (95%CI: 0.36–6.3) had positive immune serology. SS patients had lower lymphocyte, hemoglobin and C3 levels but higher prevalence of RF, ANA, anti-SSA/B and higher IgG and MFI levels, compared to DES and controls (P<0.001). Presence of anti-SSA/Ro-52kD was associated with SS [odds ratio (OR) = 2.05, 95% confidence interval (CI): 1.46–2.88]. Anti-SSB/La was inversely associated with DES (OR = 0.81, 95%CI: 0.65–1.00) compared to controls. Positivity to RF (adjusted for age, gender and ethnicity OR = 5.03, 95%CI: 1.78–14.21), ANA (OR = 14.75, 95%CI: 4.09–53.17), or combination of anti-SSA/B (OR = 20.97, 95%CI: 4.60–95.54) were more likely in SS compared to DES. The novel multiplex-serology method correctly identified anti-SSA/B autoantibodies by ELISA among SS, DES patients and controls (sensitivity = 1.0, negative-predictive-value = 1.0). Conclusions Serologic parameters distinguish SS from DES patients and controls. A newly-developed multiplex-serology technique may be useful to detect autoantibodies in large epidemiologic studies.

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SNP variations in IL10, TNFα and TNFAIP3 genes in patients with dry eye syndrome and Sjogren’s syndrome

Journal of Inflammation ◽

10.1186/s12950-019-0209-z ◽

2019 ◽

Vol 16 (1) ◽

Cited By ~ 2

Author(s):

Hadas Ben-Eli ◽

Nir Gomel ◽

Doron Jacob Aframian ◽

Rania Abu-Seir ◽

Riki Perlman ◽

...

Keyword(s):

Sjögren’S Syndrome ◽

Dry Eye ◽

Sjögren's Syndrome ◽

Sjogren’S Syndrome ◽

Sjogren's Syndrome ◽

Dry Eye Syndrome ◽

Sjögren‘S Syndrome

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Comprehensive Treatment of Dry Eye Syndrome in Patients with Sjogren’s Syndrome by Using Preservative-Free Drops Containing 0.4 % Sodium Hyaluronate and a Complex of Nutraceuticals

International scientific-practical journal Ophthalmology ◽

10.30702/ophthalmology27082021-14.3.08-17/002.1-022 ◽

2021 ◽

pp. 8-17

Author(s):

Oleksandra V. Zborovska ◽

Ilijna S. Horyanova ◽

Victoria V. Kolesnichenko

Keyword(s):

Sjögren’S Syndrome ◽

Dry Eye ◽

Sjögren's Syndrome ◽

Sodium Hyaluronate ◽

Sjogren’S Syndrome ◽

Sjogren's Syndrome ◽

Dry Eye Syndrome ◽

Comprehensive Treatment ◽

Preservative Free ◽

Sjögren‘S Syndrome

The aim. To increase the effectiveness of treatment of dry eye syndrome in patients with Sjogren’s syndrome with preservative-free drops which contain 0.4 % sodium hyaluronate and nutraceutical complexes nutraceuticals. Materials and methods. The study was conducted on the basis of the Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine. The study included 64 patients aged 34 to 58 years (128 eyes) with dry eye syndrome accompanied by Sjogren’s syndrome. Examination of patients included: assessment of complaints, visometry, refractometry, bacterial culture of the conjunctiva and nasopharynx, biomicroscopy, ophthalmoscopy, Schirmer and Norn tests, assessment of "comfort of application of drops" on the questionnaire. This study is an extension of the study previously conducted at the premises of the Filatov Institute of Eye Diseases and Tissue Therapy of The National Academy of Medical Sciences of Ukraine on the effectiveness of preservative-free eye drops containing 0.4 % hyaluronic acid in the treatment of Sjogren’s syndrome. Results. Prior to the appointment of comprehensive treatment with instillations, the Norn test showed 6.55 s (SD 1.57) with a minimum of 3 s, a maximum of 9 s, and a median of 6.5 s. After 3 months of treatment the Norn test score in the 1st group averaged 14.66 (SD 1.07) vs. 13.86 (SD 1.07) in the 2nd group, but the difference was not statistically significant (p=1.0). The mean number of required instillations per day was 4.1 (SD 1.04) in the 1st group and 5.27 (SD 1.11) in the 2nd group. Conclusion. Instillations of preservative-free drops containing sodium hyaluronate 0.4 % (in a bottle with an innovative ophthalmic squeeze dispenser (OSD)) in combination with a nutraceutical complex in the treatment of dry eye syndrome in patients with Sjogren’s syndrome is effective and allows achieving a stable and long-term effect. Keywords: Sjogren’s syndrome, hyaluronic acid, nutraceuticals, artificial tear, Schirmer test, Norn test, dry eye syndrome, tear film.

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Increasing the Efficiency of Treatment of Dry Eye Syndrome in Patients with Sjogren’s Syndrome by Application of the Preparation of Preserved Drops Containing Sodium Hyaluronate 0.4%

International scientific-practical journal Ophthalmology ◽

10.30702/ophthalmology30092020-11.1.1615-22 ◽

2020 ◽

pp. 15-22

Author(s):

A Zborovskaya ◽

A Dorokhova ◽

V Kolesnichenko

Keyword(s):

Sjögren’S Syndrome ◽

Dry Eye ◽

Sjögren's Syndrome ◽

Sodium Hyaluronate ◽

Sjogren’S Syndrome ◽

Sjogren's Syndrome ◽

Dry Eye Syndrome ◽

Tissue Therapy ◽

Study Results ◽

Sjögren‘S Syndrome

Purpose. To increase the effectiveness of the treatment of dry eye syndrome in patients with Sjogren’s syndrome by using the drug without preservative drops containing sodium hyaluronate 0.4%. Materials and methods. The research was carried out on the basis of “The Filatov Institute of Eye Diseases and Tissue Therapy of The National Academy of Medical Sciences of Ukraine”. The study included 14 patients aged 34 to 59 years (28 eyes) with dry eye syndrome concomitant with Sjogren’s syndrome. The examination of patients included: assessment of complaints, visometry, refractometry, culture tank from the conjunctiva, biomicroscopy, ophthalmoscopy, Schirmer’s test and Norn’s test, assessment of the “comfort of using drops” according to the questionnaire developed for this study. Results. Before the appointment of instillations, Norn’s test was 6.5 seconds (SD 1.56) with a minimum of 3 seconds and a maximum of 9 seconds, a median of 6.5 seconds. After a month of treatment while taking drops, Norn’s test in the first group averaged 14.86 (SD 1.07) versus 13.86 (SD 1.07), but the difference was not statistically significant (p=1.0). The average number of instillations required per day for the first group was 4.14 (SD 1.06) and for the second group it was: 5.29 (SD 1.11). Conclusion. Instillations of non-preservative drops containing sodium hyaluronate 0.4% (in a bottle equipped with an innovative reusable push-type ophthalmic dispenser (OSD) in the treatment of dry eye syndrome in patients with Sjogren’s syndrome are quite effective and very convenient to use.

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