The Avidin-Biotin Complex(ABC) Method

1994 ◽  
pp. 175-184 ◽  
Author(s):  
Gary L. Bratthauer
Keyword(s):  
Abc Method ◽  
Avidin Biotin Complex

scholarly journals Immunohistochemical detection of soya protein – optimisation and verification of the method

2009 ◽  
Vol 27 (No. 1) ◽  
pp. 11-19 ◽  
Author(s):  
M. Pospiech ◽  
B. Tremlová ◽  
E. Renčová ◽  
Z. Randulová
Keyword(s):  
Indirect Elisa ◽  
Soya Protein ◽  
Immunohistochemical Method ◽  
Immunohistochemical Detection ◽  
Elisa Method ◽  
Background Staining ◽  
Soya Proteins ◽  
Abc Method ◽  
Soya Isolate ◽  
Avidin Biotin Complex

A functional immunohistochemical method for soya proteins detection was developed. The procedure is based on the avidin-biotin complex (ABC) method that attains sufficient sensitivity. The method was verified by the analysis of the model samples of different forms of soya additives containing various concentrations of soya isolate. The detection limit of soya present in the model samples was 0.5%. Different possibilities of the background staining were tested. The best results were obtained with the background staining according to Calleja. The results were confirmed by the accredited indirect ELISA method. The method allows the identification of various forms of soya proteins such as isolates, texturates, concentrates, and flour.

scholarly journals Evaluation of Immunohistochemical Reactivity with Avidin-Biotin Complex (ABC) Method

2018 ◽  
Vol 3 (3) ◽  
pp. 909-918
Author(s):  
C. Sandoval ◽  
B. Vásquez
Keyword(s):  
Malignant Melanoma ◽  
Primary Antibody ◽  
Histopathological Diagnosis ◽  
Distilled Water ◽  
Correct Interpretation ◽  
S 100 ◽  
Antibody Reaction ◽  
Abc Method ◽  
Antigen Antibody ◽  
Avidin Biotin Complex

Immunohistochemistry is any technique that can detect cellular and extracellular components in situ by means of specific antibodies, using enzymatic detection systems. Among immunohistochemical methods, the technique of avidin - biotin complex (ABC) is widely used because of its high sensitivity. The aim of this study was to evaluate the immunohistochemical reactivity of the 4C4.9 antibody for detection of S-100 protein using the ABC method. For the evaluation of immunohistochemical reactivity 2 biopsies of human skin were used with histopathological diagnosis of ulcerated malignant melanoma and melanocytic intradermal nevi from the Research Laboratory on Animal Biotechnology of the Universidad de La Frontera, Chile. The Kit VECTASTAIN® was used as detection method, the dilution the 4C4.9 antibody was 1/250 and incubation temperature was at 4 ° C or 37 °C for 18 hours. To validate the technique, a positive control and a negative for 4C4.9 was performed. The results of immunohistochemical staining by the method of ABC complex showed positive staining for protein S-100 both in ulcerated malignant melanoma and melanocytic intradermal nevi, incubated for 18 hours at 4 ° C or 37 ° C. However, immunostaining was more intense when the primary antibody was incubated at 37° C. For a correct interpretation of the results, it is necessary to take into consideration that the antigen-antibody reaction is influenced by various factors such as the concentration of antibody, time and temperature of incubation. In conclusion, our results suggest incubating the samples with the first antibody (4C4.9) at 1/250 dilution in distilled water, incubating for 18 h at 37 oC. However, immunostaining was more intense when the primary antibody was incubated at 37° C. For a correct interpretation of the results, it is necessary to take into consideration that antigen- antibody reaction is influenced by various factors such as the concentration of antibody, time and temperature of incubation. In conclusion, our results suggest incubating the samples with the first antibody (4C4.9) at 1/250 dilution in distilled water, incubating for 18 h at 37 oC. The use of the antibody 4C4.9 is recommended to support the diagnosis and differential diagnosis.

The demonstration of terminal deoxynucleotidyl transferase on frozen tissue sections and smears by the avidin-biotin complex (ABC) method

1983 ◽  
Vol 7 (3) ◽  
pp. 431-437 ◽  
Author(s):  
Barbara Racklin ◽  
Robert Bearman ◽  
Khalil Sheibani ◽  
Carl Winberg ◽  
Henry Rappaport
Keyword(s):  
Terminal Deoxynucleotidyl Transferase ◽  
Tissue Sections ◽  
Frozen Tissue ◽  
Abc Method ◽  
Avidin Biotin Complex

Localization of bombesin at the electron microscope level employing the modified ABC method in the rat neostriatum

1984 ◽  
Vol 42 ◽  
pp. 248-249
Author(s):  
Ronald H. Bradley
Keyword(s):  
Electron Microscope ◽  
Substance P ◽  
Survival Time ◽  
Frog Skin ◽  
Microscope Level ◽  
Electron Microscope Level ◽  
Methionine Enkephalin ◽  
Intraventricular Injections ◽  
Abc Method ◽  
Avidin Biotin Complex

Bombesin was originally isolated from frog skin (1) and it has been recently localized with the CNS (2). Bombesin has been shown to bind certain pharmacological drugs such as diazepams within the CNS (3). Recent investigations by our lab have been successful in localizing other putative neurotransmitter candidates such as methionine enkephalin, leucine enkephalin, substance P and GAD with the modified avidin-biotin complex technique within rat neostriatum (4). The present study was designed to localize bombesin in somata of neostriatal neurons in rats that were treated with colchicine to increase somatal neurotransmitter concentrations.The increase of somatal bombesin, a putative neurotransmitter, was accomplished by bilateral stereotaxic intraventricular injections of 15 µ1 (10 µ1/µg) colchicine per ventricle. Following a 48 hr survival time, the rats were intracardially perfused with our modified immunocytochemical buffer (3) which contains 1% glutaraldehyde, 1% paraformaldehyde in a microtubule stabilizing buffer (MSB) at pH 7.1 (37°C).

Detection of infectious laryngotracheitis virus in trigeminal ganglia by avidin-biotin complex method in chickens: Short communication

2004 ◽  
Vol 52 (2) ◽  
pp. 167-171 ◽  
Author(s):  
F. Yilmaz ◽  
N. Timurkaan ◽  
H. Bulut
Keyword(s):  
Latent Period ◽  
Trigeminal Ganglia ◽  
Complex Method ◽  
Post Inoculation ◽  
Infectious Laryngotracheitis Virus ◽  
Tissue Samples ◽  
Infectious Laryngotracheitis ◽  
Abc Method ◽  
Laryngotracheitis Virus ◽  
Avidin Biotin Complex

Detection of infectious laryngotracheitis virus (ILTV) by avidin-biotin complex (ABC) method was studied in trigeminal ganglia (TRG) during the acute and post-acute or latent period in chickens inoculated with an A96 strain of the antigen. TRG tissue samples were collected from uninoculated (10 chickens) and inoculated (30 chickens) animals at various intervals post inoculation (PI), and stained by the ABC method. The results indicated that no ILTV antigen was detected in the TRG between PI days 3 and 11. However, the antigen was detected in most of the chickens between PI days 13 and 41. It is concluded that the ABC method can be used successfully for the detection of ILTV antigens in TRG during post-acute or latent period.

scholarly journals The unlabeled antibody method: comparison of peroxidase-antiperoxidase with avidin-biotin complex by a new method of quantification.

1986 ◽  
Vol 34 (5) ◽  
pp. 599-605 ◽  
Author(s):  
L A Sternberger ◽  
N H Sternberger
Keyword(s):  
Method Comparison ◽  
Staining Intensity ◽  
The Other ◽  
Tissue Sections ◽  
Low Concentrations ◽  
Pap Method ◽  
Abc Method ◽  
Straight Lines ◽  
Unlabeled Antibody ◽  
Avidin Biotin Complex

Upon plotting of areas against optical densities in immunocytochemically stained tissue sections, hyperbolic curves were obtained which could be reduced to two straight lines, one representing variations in stained structures, and the other variations in background. The slopes of the stained structure lines reflected staining intensity independently of total area of stained structure in a section. The ratio of slopes of the stained structure and background lines reflected immunocytochemical sensitivity. A comparison of the peroxidase-antiperoxidase (PAP) method with the avidin-biotin complex (ABC) method showed that at usual antibody dilutions the PAP method was much more sensitive than the ABC method, while at impractically high antibody dilutions it was moderately more sensitive. Once sufficient dilutions of antibodies were reached, staining intensities dropped sharply with the PAP method. On the other hand, the dilution curves were flat with the ABC method. The ABC method consequently appeared unsuitable for estimating variations in concentration of antigen or for distinguishing high or low concentrations of antigen. The ABC method provided a stain for myelin even in the absence of any antibodies.

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