scholarly journals Immunohistochemical detection of soya protein – optimisation and verification of the method

2009 ◽  
Vol 27 (No. 1) ◽  
pp. 11-19 ◽  
Author(s):  
M. Pospiech ◽  
B. Tremlová ◽  
E. Renčová ◽  
Z. Randulová
Keyword(s):  
Indirect Elisa ◽  
Soya Protein ◽  
Immunohistochemical Method ◽  
Immunohistochemical Detection ◽  
Elisa Method ◽  
Background Staining ◽  
Soya Proteins ◽  
Abc Method ◽  
Soya Isolate ◽  
Avidin Biotin Complex

A functional immunohistochemical method for soya proteins detection was developed. The procedure is based on the avidin-biotin complex (ABC) method that attains sufficient sensitivity. The method was verified by the analysis of the model samples of different forms of soya additives containing various concentrations of soya isolate. The detection limit of soya present in the model samples was 0.5%. Different possibilities of the background staining were tested. The best results were obtained with the background staining according to Calleja. The results were confirmed by the accredited indirect ELISA method. The method allows the identification of various forms of soya proteins such as isolates, texturates, concentrates, and flour.

scholarly journals Immunohistochemical detection of wheat protein in model samples

2010 ◽  
Vol 28 (No. 6) ◽  
pp. 516-519 ◽  
Author(s):  
Z. Řezáčová-Lukášková ◽  
B. Tremlová ◽  
M. Pospiech ◽  
E. Renčová ◽  
Z. Randulová
Keyword(s):  
Polyclonal Antibodies ◽  
Protein Detection ◽  
Immunohistochemical Method ◽  
Complex Method ◽  
Pork Meat ◽  
Immunohistochemical Detection ◽  
Wheat Protein ◽  
Elisa Method ◽  
Immunohistochemical Examination ◽  
Gluten Content

The study focused on the optimisation of immunohistochemical examination for gluten content detection in model samples (pork meat with wheat semi-smooth flour, pork meat with wheat protein edible vital). The best results were achieved with immunohistochemical method based on ABC (avidin–biotin complex) method utilising polyclonal antibodies diluted 1:1000. The results demonstrate that for pure wheat protein detection, the utilisation of immunohistochemical detection, which can detect as little as 0.1% of the added wheat protein, is more advantageous, while the commonly used ELISA method reliably proves this additive approximately from 0.4% upwards.

scholarly journals Comparison of Trichomoniasis Diagnosis by Microscopic Methods and Indirect ELISA Technique in a Sample of Iraqi Women

2020 ◽  
pp. 742-748
Author(s):  
Nada H. Bedair ◽  
Hayder Z. Ali
Keyword(s):  
Trichomonas Vaginalis ◽  
Vaginal Discharge ◽  
Indirect Elisa ◽  
Elisa Method ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
Wet Mount ◽  
Urine Examination ◽  
Iraqi Women ◽  
Elisa Technique

Trichomonas vaginalis is an eukaryotic parasite that causes the most common non-viral sexually transmitted infection, trichomoniasis. This disease is responsible for many serious health problems such as preterm birth. More than half of the infected women do not develop symptoms, which makes it difficult to diagnose thedisease. In this study, a specific indirect ELISA method was developed to detect anti-Trichomonas vaginalis IgM and IgG immunoglobulins in the sera of infected females. The aim of this study was to investigate the sensitivity of a simple ELISA procedure in comparison to the classical urine examination and vaginal wet mount preparation for the diagnosis of T. vaginalis. The sensitivity of the indirect ELISA was compared with the classical vaginal discharge swab and urine microscopic examination, and the results showed sensitivities of 65.5% and 57.2%, respectively. Furthermore, the infection was measurable as acute or chronic with the refined test.

Evaluation of an enzymatically treated soya protein in the diet of weaned pigs

1994 ◽  
Vol 1994 ◽  
pp. 160-160
Author(s):  
C A Weeks ◽  
BG Miller ◽  
L Green ◽  
S Rizvi ◽  
C Beck ◽  
...  
Keyword(s):  
Milk Proteins ◽  
Protein Isolate ◽  
Soya Protein ◽  
Test Diet ◽  
Soya Protein Isolate ◽  
Commercial Farms ◽  
Main Protein ◽  
Soya Proteins ◽  
Set Up ◽  
Experimental Pigs

Soya proteins can provoke an immune response and cause diarrhoea in pigs weaned at 3 weeks of age. An enzymatically treated soya protein isolate (NURISH protein) did not have these effects in isolator reared experimental pigs (Miller et al 1992). A blind trial on 4 commercial farms was set up to evaluate NURISH as an alternative to conventional protein sources.On each farm 120 pigs (in 2 or 3 replicates) were fed either a typical commercial weaner diet containing cereals, fishmeal, milk proteins and full fat soya (the control) or a test diet matched for protein (22% CP), lysine (1.6%) and energy (DE 15.1 MJ/kg) with NURISH as the main protein source. Prophylactic antibiotics were not used.

scholarly journals Comparison of the results of the ELISA, histochemical, and immunohistochemical detection of soya proteins in meat products    

2011 ◽  
Vol 29 (No. 5) ◽  
pp. 471-479 ◽  
Author(s):  
M. Pospiech ◽  
B. Tremlová ◽  
E. Renčová ◽  
RandulováZ ◽  
Z. Řezáčová Lukášková ◽  
...  
Keyword(s):  
Meat Products ◽  
Protein Detection ◽  
Soya Protein ◽  
Microscopical Examination ◽  
Immunochemical Method ◽  
Immunohistochemical Methods ◽  
Immunochemical Methods ◽  
Soya Proteins ◽  
The Individual ◽  
Comparison Of The Results

This work compares the commonly used immunochemical methods for soya protein detection and alternative microscopic methods. Immunochemical methods were represented by the competitive ELISA method. Histochemical and immunohistochemical methods were used for microscopical examination. From a group of 252 meat products, each sample was examined for soya proteins by ELISA, histochemical, and immunohistochemical methods. The products came from the following categories: cooked sausages, ham, dry cooked sausages, and fermented sausages. The results showed that the highest accuracy was achieved by immunohistochemical examination. However, in the category of cooked sausages, this result was not statistically significant. Since the results in the individual categories differed, our results demonstrate that one single method does not always provide reliable and completely objective results. Immunohistochemical methods seem to be the most suitable for the verification of the reference immunochemical method results and prevention of false results.

Monoclonal Antibody Detection of Porcine Meat

1994 ◽  
Vol 57 (2) ◽  
pp. 146-149 ◽  
Author(s):  
P. MORALES ◽  
T. GARCÍA ◽  
I. GONZÁLEZ ◽  
R. MARTÍN ◽  
B. SANZ ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Bovine Serum Albumin ◽  
Horseradish Peroxidase ◽  
Indirect Elisa ◽  
Cross Reactivity ◽  
Antibody Detection ◽  
Muscle Proteins ◽  
Elisa Format ◽  
Soya Proteins

A stable hybridoma cell line (DD9) has been produced secreting a monoclonal antibody specific for porcine muscle proteins. The DD9 monoclonal antibody (mAb) failed to show a significant cross-reactivity when tested against beef, horse, chicken, and soya proteins, as well as bovine caseins, gelatin, and bovine serum albumin. The DD9 mAb was further used in an indirect ELISA format for detection of defined amounts of porcine meat (1–100%) in beef and chicken meat mixtures immobilized on 96-well plates. Immunorecognition of monoclonal antibodies adsorbed to porcine meat was made with rabbit anti-mouse immunoglobulins conjugated to the enzyme horseradish peroxidase.

scholarly journals An Improved Protocol of Biotinylated Tyramine-based Immunohistochemistry Minimizing Nonspecific Background Staining

2003 ◽  
Vol 51 (1) ◽  
pp. 129-132 ◽  
Author(s):  
Seok Hyung Kim ◽  
Young Kee Shin ◽  
Kyung Mee Lee ◽  
Jung Sun Lee ◽  
Ji Hye Yun ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Blocking Agent ◽  
Immunohistochemical Method ◽  
Tween 20 ◽  
Distilled Water ◽  
Blocking Agents ◽  
Weak Staining ◽  
Optimized Protocol ◽  
Background Staining

An immunohistochemical method using biotinyl tyramine was recently introduced to amplify weak staining signals. Despite its high sensitivity, however, tyramine-based immunostaining has been limited by its increased background staining. In this study, to develop an improved protocol of biotinyl tyramine-based immunohistochemistry minimizing the background staining, we determined which staining steps lead to the nonspecific reaction and the most appropriate blocking agents for background-provoking steps. Trypton casein peptone and distilled water with Tween-20 were shown to be most effective as a blocking agent and a rinsing solution, respectively. In conclusion, we developed an optimized protocol for biotinyl tyramine-based immunohistochemistry with minimal background staining.

scholarly journals Immunohistochemical detection of human brown adipose tissue uncoupling protein in an autopsy series.

1993 ◽  
Vol 41 (5) ◽  
pp. 759-764 ◽  
Author(s):  
M L Kortelainen ◽  
G Pelletier ◽  
D Ricquier ◽  
L J Bukowiecki
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Uncoupling Protein ◽  
Immunohistochemical Method ◽  
Histopathological Diagnosis ◽  
Positive Staining ◽  
Post Mortem ◽  
Immunohistochemical Detection ◽  
Intense Staining ◽  
Brown Adipose

We used an immunohistochemical method for the inner mitochondrial membrane uncoupling protein (UCP) to examine whether human brown adipose tissue UCP could be detected by an anti-rat UCP antibody. Samples of human brown adipose tissue were obtained at medicolegal autopsies. Fat tissue was excised from around the common carotid arteries and in the subscapular region and from around the thoracic aorta. The subjects were either known alcohol consumers, in which thermogenically active brown adipose tissue (BAT) is often found, or victims of sudden infant death syndrome (SID). UCP was detected in all the cases examined, even when the post-mortem time from death to autopsy reached several days, but the intensity of the immunostaining was variable. Intense staining was observed in three cases with a post-mortem time under 24 hr, but in the SID cases a strong positive staining was seen even with a post-mortem delay of 4 days. These results show that an anti-rat UCP antibody can be used for immunohistochemical detection of UCP in human brown adipose tissue and that it provides a useful method for distinguishing between white and brown fat in paraffin-embedded samples. It can be used to detect UCP in the BAT of obese and diabetic individuals and probably also in the histopathological diagnosis of brown adipose tissue lipoma, known as hibernoma.

scholarly journals INVESTIGATION OF FUNCTIONAL-TECHNOLOGICAL PROPERTIES OF SOYA PROTEIN

2018 ◽  
Vol 5 ◽  
pp. 39-43
Author(s):  
Oksana Kochubei-Lytvynenko ◽  
Olha Yatsenko ◽  
Nataliia Yushchenko ◽  
Ulyana Kuzmyk
Keyword(s):  
Protein Isolate ◽  
Soya Protein ◽  
Variable Velocity ◽  
Technological Properties ◽  
Technological Parameters ◽  
Soya Protein Isolate ◽  
Process Duration ◽  
Limit Stress ◽  
Storage Term ◽  
Soya Isolate

There was offered and grounded the use of functional technological properties of the soya protein isolate in the technology of oil pasts. It will allows to increase the balance of the oil past composition additionally and will favor the decrease of extracting moisture during the storage term. There was studied the dynamics of a gradient of the limit stress of soya protein: hydrated soya protein, hydrated soya protein with the temperature processing, hydrated soya protein with the preliminary keeping during 24 h, hydrated soya protein with the preliminary keeping during 24 h and temperature processing during 5 min, hydrated soya protein with the preliminary keeping during 24 h and temperature processing during 10 min; hydrated soya protein with the preliminary keeping during 24 h and temperature processing during 15 min. It was established, that the hydrated isolate of soya protein is a plastic system, has enough strength. The limit stress parameter at the variable velocity of deformation of model samples determines optimal technological parameters of preparing the soya isolate: hydromodule – 1: 8, temperature processing – (82±2) °С, process duration 10 min with preliminary keeping during 24 hours. As a result of the studies, there were demonstrated technological parameters of preparing the soya protein isolate for obtaining the oil past by the direct mixing with the oil base.

scholarly journals Antibody response of beef calves to experimental monovalent and multivalent inactivated bovine viral diarrhoea virus vaccines as measured by indirect elisa method

2011 ◽  
Vol 27 (3) ◽  
pp. 901-911 ◽  
Author(s):  
V. Kurcubic ◽  
T. Petrovic ◽  
R. Djokovic ◽  
Z. Ilic ◽  
M.D. Petrovic
Keyword(s):  
Indirect Elisa ◽  
Bovine Viral Diarrhoea Virus ◽  
Control Group ◽  
Bovine Viral Diarrhoea ◽  
Elisa Method ◽  
Animal Group ◽  
Beef Calves ◽  
Group I ◽  
Test Kit ◽  
Diarrhoea Virus

The objective of this study was to determine the immunogenic properties of two experimental inactivated (mono - and multivalent) vaccines containing BVDV type 1 reference strains (NADL, W1. - 162903, W2. - 172984, W3. - 173481, W4. - 179725) and one local field isolate derived from a calf suffering from mucosal disease (MD). Normal healthy beef calves (Simmental race) of mixed sex, 6 to 7 months of age, were divided into three experimental groups: ten calves vaccinated twice (days 1 and 28) subcutaneously (s/c) with 2 ml of inactivated multivalent vaccine per animal (Group I); ten calves vaccinated twice (days 1 and 28) subcutaneously (s/c) with 2 ml of inactivated monovalent vaccine per animal (Group II) and 9 unvaccinated calves (Control group C). Blood sera were obtained from immunized animals (standard procedure: on days 0, 14, 28, 42 and 56 post-immunization). The immune response to BVDV vaccine strains was assessed by the indirect ELISA method (Bovine Viral Diarrhoea Virus Antibody Test Kit BVDV HerdChek

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