Typing of Y Chromosome SNPs With Multiplex PCR Methods

2004 ◽  
pp. 209-228 ◽  
Author(s):  
Juan J. Sanchez ◽  
Claus Børsting ◽  
Niels Morling
Keyword(s):  
Multiplex Pcr ◽  
Y Chromosome

Noninvasive genotyping of 9 Y-chromosome specific STR loci using circulatory fetal DNA in maternal plasma by multiplex PCR

2006 ◽  
Vol 26 (4) ◽  
pp. 362-368 ◽  
Author(s):  
ZhiHui Deng ◽  
GuoGuang Wu ◽  
Qian Li ◽  
Xuan Zhang ◽  
YanLian Liang ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Y Chromosome ◽  
Maternal Plasma ◽  
Str Loci ◽  
Fetal Dna

Evaluation of a Multiplex PCR Kit Used for Detecting Y Chromosome Microdeletions

2010 ◽  
Vol 30 (4) ◽  
pp. 432-439
Author(s):  
Mi Young Park ◽  
Hye-Min Kang ◽  
Sang-Hyun Hwang ◽  
Hyun-Jun Park ◽  
Nam-Cheol Park ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Y Chromosome ◽  
Y Chromosome Microdeletions

Early non-invasive detection of fetal Y chromosome sequences in maternal plasma using multiplex PCR

2012 ◽  
Vol 161 (1) ◽  
pp. 34-37 ◽  
Author(s):  
Aggeliki Kolialexi ◽  
Georgia Tounta ◽  
Paraskevi Apostolou ◽  
Christina Vrettou ◽  
Nikos Papantoniou ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Y Chromosome ◽  
Maternal Plasma ◽  
Non Invasive

scholarly journals Establishment of a Multiplex – PCR protocol for detection of Y chromosome microdeletion in Azoospermia male patients

2015 ◽  
Vol 18 (4) ◽  
pp. 5-15
Author(s):  
Phuong Thi Kim Huynh ◽  
Thanh Kieu Huynh ◽  
Nam Tri Vo ◽  
Anh Le Tuan Nguyen ◽  
Hoang Duc Nguyen ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Y Chromosome ◽  
Control Sample ◽  
Current Approach ◽  
Marker Genes ◽  
Dna Electrophoresis ◽  
Azoospermia Factor ◽  
Multiplex Pcr Assay ◽  
Pcr Products ◽  
Male Patients

Microdeletion on the Y chromosome is one of the causes that makes men infertile, accounting for 2-10 % of all infertility cases, and occurs frequently at 3 regions of the Ychromosome long arm namely AZFa, AZFb and AZFc (azoospermia factor). Currently, the diagnosis of microdeletion on the Y chromosome is almost mandatory in institutes and centers for infertility diseases before selecting treatment or assisting methods. To detect microdeletion in AZF, SRY and ZFY regions, the current approach is a Multiplex – PCR assay offering by European Academy of Andrology/European Molecular Genetics Quality Network (EAA/ EMQN). However, the drawback of this method is the PCR products posess similar size and then the DNA electrophoresis bands were very close on gels causing the difficult in diagnosis. Therefore, in this study, we have redesigned primer pairs matching with genes that were recommended by EAA/EMQN but the PCR products are clearly different in sizes, making the DNA electrophoresis bands take apart further to facilitate the diagnosis. Besides, we have also created recombinant plasmids carrying the marker genes for the control sample in kits.

scholarly journals Study of Y-Chromosome Microdeletions in Azoospermic Infertile Males using Multiplex PCR Analysis

2018 ◽  
Vol 15 (2) ◽  
pp. 351-357 ◽  
Author(s):  
Prafulla S. Ambulkar ◽  
Sunil S. Pande
Keyword(s):  
Multiplex Pcr ◽  
Y Chromosome ◽  
Pcr Analysis ◽  
Obstructive Azoospermia ◽  
Time Saving ◽  
Pcr Screening ◽  
Azoospermia Factor ◽  
Sts Markers ◽  
Y Chromosome Microdeletions ◽  
Severe Impairment

The infertility affects about 15% of couples and male factors being responsible about 40-50%. In male infertility, genetic abnormalities of Y chromosome play crucial role in spermatogenesis defect. Y chromosome q arm having Azoospermia factor region (AZFa, AZFb, and AZFc) are most important for spermatogenesis. Here, we investigated the frequencies of Y-chromosome microdeletions using three sets of multiplex PCR in idiopathic cases of azoospermia. We studied a total of 110 infertile male with non-obstructive azoospermia subjects & 50 fertile control subjects. All DNA samples were used for Y chromosome microdeletions analysis by using 11 STS markers in three different multiplex PCR of AZF regions. Out of 110 infertile azoospermic males, 14 (12.72%) infertile males showed partial deletion of AZF regions using three sets of multiplex PCR group. In the AZF microdeletions of infertile males, individually AZFc region was the most deletions sites (10%) followed by AZFb (6.36%) and AZFa (1.81%). The sites and sizes of microdeletions differ in all infertile azoospermic males who showed at least two or more STS markers microdeletions. The frequency of Y chromosome microdeletions in our azoospermic infertile males is 12.72%. We conclude that Y chromosome microdeletions frequency in azoospermic infertile males is higher than other infertile group due to severe impairment in spermatogenesis. Multiplex PCR screening of microdeletions is very useful and time saving technique when used more number of STS markers. It will be great help to infertility clinics for genetic counseling and assisted reproduction.

Y chromosome SNP analysis in a Portuguese population sample using a multiplex PCR and minisequencing strategy

2008 ◽  
Vol 1 (1) ◽  
pp. 190-191
Author(s):  
M. Carvalho ◽  
A.M. Bento ◽  
L. Andrade ◽  
M.J. Anjos ◽  
V. Lopes ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Y Chromosome ◽  
Population Sample ◽  
Snp Analysis ◽  
Portuguese Population

scholarly journals Molecular Markers for Human Sex Determination in Forensic Genetics Analysis

2021 ◽  
Vol 8 (6) ◽  
pp. 25-30
Author(s):  
Maan H. Salih
Keyword(s):  
Sex Determination ◽  
Multiplex Pcr ◽  
Genetic Marker ◽  
Y Chromosome ◽  
Internal Control ◽  
Forensic Genetics ◽  
Sex Identification ◽  
Pcr Analysis ◽  
Positive Band ◽  
Two Samples

Sex determination is indispensable in forensic anthropology, sexual disorder, and also as part of large-scale genetic population studies. The purpose of this investigation is to determine the human sex from whole blood using multiplex PCR analysis. Blood samples from 75 male and 70 female healthy volunteers were taken from Tikrit city, Iraq. Our study identified a reliable set of three primer locus, namely SRY, ALT1 (internal control) and amelogenin locus. The SRY primer on the Y chromosome showed a 254 bp of PCR product, with 100% accuracy for human male identification. Thus, the pair of SRY primers was considered a strong genetic marker for human sex identification. Amelogenin regions in the Y chromosome showed a true positive band (236 bp) with 100% accuracy on sex identification. Amelogenin regions in X chromosome also showed positive bands (330 bp) in female samples and positive band in male samples except for two samples showed a negative band (null bands). The most obvious finding from this study is that multiplex PCR of ALT1 and SRY is consider as a reliable genetic marker for human sex identification. The research has also shown that amelogenin is good genetic marker for human sex identification.

scholarly journals A rapid and simple system of detecting deletions on the Y chromosome related with male infertility using multiplex PCR

2006 ◽  
Vol 53 (1,2) ◽  
pp. 147-152 ◽  
Author(s):  
Mayumi Umeno ◽  
Toshikatsu Shinka ◽  
Youichi Sato ◽  
Xin-Jun Yang ◽  
Yoshinobu Baba ◽  
...  
Keyword(s):  
Male Infertility ◽  
Multiplex Pcr ◽  
Y Chromosome ◽  
Simple System
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