Background & Objective:
NSCs therapy is considered one of the most potential methods
for spinal cord injury (SCI).
Methods:
We build the SCI model rats to investigate the therapeutic effect of fire needle acupuncture
in improving the locomotor function of SCI rats and its possible mechanism. BBB scale was used for
the motor ability of rats. The expression of Nestin, NSE, Gal-C, and GFAP was detected by immunohistochemistry.
Wnt, GSK3β, β-catenin, ERK1/2, CyclinD1, and ngn1 were detected by western blot
and PCR. The BBB score of both model group (1.20±0.94, 3.12±0.67, 5.34±1.57, 7.12±1.49) and fire
needle group (1.70±0.58, 4.50±1.63, 7.53±2.41, 9.24±0.63) gradually increased after SCI. Furthermore,
at d10 and d14, the fire needle group showed a significantly high score compared with that in
model group at the same time (P<0.05). Fire needle increased Nestin, NSE, and Gal-C expression inhibited
GFAP expression after SCI. Also, fire needle could up-regulate Wnt3a, GSK3β, β-catenin, and
ngn1, and down-regulate ERK1/2, cyclinD1 gene and protein expression.
Conclusion:
In conclusion, fire needle could improve lower limb locomotor function of SCI rats. Also,
fire needles could promote endogenous NSCs proliferation differentiating into neurons, and the
mechanism might be mediated by promoting the activation of Wnt/β-catenin and inhibiting the overexpression
of ERK.
Case Report - Fire-Needle Acupuncture Treatment In Skin Papillomas Located on the Neck
