Determinants of membrane protein topology and membrane anchoring

1998 ◽  
pp. 49-66 ◽  
Keyword(s):  
Membrane Protein ◽  
Protein Topology ◽  
Membrane Protein Topology ◽  
Membrane Anchoring

scholarly journals SARS-CoV-2 envelope protein topology in eukaryotic membranes

Open Biology ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 200209 ◽  
Author(s):  
Gerard Duart ◽  
Mª Jesús García-Murria ◽  
Brayan Grau ◽  
José M. Acosta-Cáceres ◽  
Luis Martínez-Gil ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Mammalian Cells ◽  
E Proteins ◽  
Virus Envelope ◽  
Protein Topology ◽  
Membrane Protein Topology ◽  
E Protein ◽  
C Terminus ◽  
Microsomal Membranes ◽  
Cytoplasmic Side

Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and host components and contribute to establish the basis to tackle the pathogenesis of SARS-CoV-2.

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