Serotyping of N. meningitidis group B strains in China with monoclonal antibodies by whole cell ELISA

1991 ◽  
pp. 11-16
Author(s):  
X.Y. Bai ◽  
L.Y. Wang ◽  
W.X. Huang ◽  
D.Z. Liu
Keyword(s):  
Monoclonal Antibodies ◽  
Whole Cell ◽  
Group B ◽  
Cell Elisa

scholarly journals Lipo-oligosaccharide immunotyping of Neisseria meningitidis by a whole-cell ELISA with monoclonal antibodies

1994 ◽  
Vol 41 (4) ◽  
pp. 236-243 ◽  
Author(s):  
R. J. P. M. SCHOLTEN ◽  
B. KUIPERS ◽  
H. A. VALKENBURG ◽  
J. DANKERT ◽  
W. D. ZOLLINGER ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Neisseria Meningitidis ◽  
Whole Cell ◽  
Cell Elisa

Serotypes and subtypes of Neisseria meningitidis serogroup B strains associated with meningococcal disease in Canada, 1977–1989

1991 ◽  
Vol 37 (8) ◽  
pp. 613-617 ◽  
Author(s):  
F. E. Ashton ◽  
L. Mancino ◽  
A. J. Ryan ◽  
J. T. Poolman ◽  
H. Abdillahi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Neisseria Meningitidis ◽  
Key Words ◽  
Outer Membrane ◽  
Meningococcal Disease ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Whole Cell ◽  
Immunosorbent Assays ◽  
Cell Elisa

Typing of Neisseria meningitidis serogroup B disease isolates was carried out using a panel of serotype- and subtype-specific monoclonal antibodies (MAbs) in enzyme-linked immunosorbent assays (ELISA). Three hundred and sixty-two strains isolated from 1977 to 1986 were typed using five serotyping and seven subtyping reagents and outer membrane vesicles as antigens. Serotype 2b accounted for 30% of the disease isolates. The most common subtype was P1.2, which occurred on 18.5% of all strains or 48.6% of the serotype 2b strains. Of the 362 strains typed, 135 (37.3%) were serotyped and 122 (33.7%) were subtyped. Overall, 185 (51.1%) of the strains could be assigned a serotype and (or) subtype. Strains (221) isolated during the years 1987–1989 were typed using a panel of 6 serotyping and 12 subtyping reagents by whole-cell ELISA. Strains of serotypes 4 (21.7%) and 15 (20.8%) were the most common and carried a wide variety of subtypes. The most common subtypes were P1.2 (11.8%) and P1.16 (9.5%). Of the 221 strains analyzed, 132 (59.7%) were assigned a serotype and 123 (55.7%) a subtype and with all 18 MAbs, 192 (86.9%) of the strains were serotyped and (or) subtyped. Two different MAbs to the four epitopes 2a, 15, P1.2, and P1.16 gave discordant reactions of 0.3, 6.6, 2.6, and 2.2%, respectively, when used to analyze over 300 strains of N. meningitidis. Key words: meningococcal serotypes, subtypes.

scholarly journals Putative Novel Surface-Exposed Streptococcus agalactiae Protein Frequently Expressed by the Group B Streptococcus from Zimbabwe

2009 ◽  
Vol 16 (9) ◽  
pp. 1302-1308 ◽  
Author(s):  
Rooyen T. Mavenyengwa ◽  
Johan A. Maeland ◽  
Sylvester R. Moyo
Keyword(s):  
Rabbit Antiserum ◽  
Periodate Oxidation ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Antigens ◽  
Group B Streptococci ◽  
Phenotypic Marker ◽  
Whole Cell ◽  
Strain Collection ◽  
Group B

ABSTRACTGroup B streptococci (GBS) express a variety of surface-exposed and strain-variable proteins which function as phenotypic markers and as antigens which are able to induce protective immunity in experimental settings. Among these proteins, the chimeric and immunologically cross-reacting alpha-like proteins are particularly important. Another protein, R3, which has been less well studied, occurred at a frequency of 21.5% in GBS from Zimbabwe and, notably, occurred in serotype V strains at a frequency of 75.9%. Working with rabbit antiserum raised against the R3 reference strain ATCC 49447 (strain 10/84; serotype V/R3) to detect the expression of the R3 protein, we recorded findings which suggested that strain 10/84 expressed a strain-variable protein antigen, in addition to R3. The antigen was detected by various enzyme-linked immunosorbent assay-based tests by using acid extract antigens or GBS whole-cell coats and by whole-cell-based Western blotting. We named the putative novel antigen the Z antigen. The Z antigen was a high-molecular-mass antigen that was susceptible to degradation by pepsin and trypsin but that was resistant tom-periodate oxidation and failed to show immunological cross-reactivity with any of a variety of other GBS protein antigens. The Z antigen was expressed by 33/121 (27.2%) of strains of a Zimbabwean GBS strain collection and by 64.2% and 72.4% of the type Ib and type V strains, respectively, and was occasionally expressed by GBS of other capsular serotypes. Thus, the putative novel GBS protein named Z showed distinct capsular antigen associations and presented as an important phenotypic marker in GBS from Zimbabwe. It may be an important antigen in GBS from larger areas of southern Africa. Its prevalence in GBS from Western countries is not known.

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