Die biologische Leistung der Hydroxylapatitkeramik-Beschichtung auf dem Femurschaft einer Titanendoprothese — erste histologische Auswertung eines Humanexplantats - The Biological Behavior of the Hydroxyapatite Ceramic Coating on a Titanium Stern of a Hip Prosthesis - the First Histological Evaluating of Human Autopsy Material

Tissue distribution of fibrin degradation products (FOP’s) during disseminated intravascular coagulation (DIC) is still largely unknown, since present immunohistochemical methods cannot discriminate between FDP’s and other fibrin-related material. Using tissue from rats with thrombin-induced DIC, we developed an immunoenzymehistochernical method for specific FDP-localization. This method is based on the following observations:1. FDP’s are - in contrast to fibrin - retained in ethanol-fixed tissue only; however, not in formaldehyde-fixed tissue;2, FDP’s in tissue will react with some of the antisera employed only (antisera against rat fibrinogen, FDP-D and E, and the constituent chains of fibrinogen were tested), whereas fibrinogen and fibrin will react with all said antisera.At 20-30 min after thrombin injection, FDP’s were found in the epithelial cells of kidney proximal tubules. These FDP’s were demonstrated with anti-chain antisera, though not with antisera against 0 or E, and are presumably early FDP’s derived from the α-and β-chain. Later (at 40-120 min), FDP’s were found in liver macrophages. These FDP’s proved unreactive to anti-chain antisera, but were demonstrated by anti-D or E antisera, A similar localization of FDP’s was found in human autopsy material in cases of DIC.

Download Full-text

Pesticides and the environment

Medicinski pregled ◽

10.2298/mpns0412523k ◽

2004 ◽

Vol 57 (11-12) ◽

pp. 523-535 ◽

Cited By ~ 2

Author(s):

Miroslava Kristoforovic-Ilic

Keyword(s):

Human Serum ◽

Surface Waters ◽

Thermal Processing ◽

Liver Fat ◽

Organochlorine Insecticide ◽

Autopsy Material ◽

Artesian Wells ◽

Novi Sad ◽

Human Autopsy Material ◽

Human Autopsy

In the period 1981-2000, an investigation was conducted on organochlorine insecticide (OCI) residues in the environment and their effects on human health. The analyses encompassed drinking and surface waters, air, foodstuffs, ready-made meals, human serum and human autopsy material. OCI residues were not detected in drinking water originating from great distributive systems in Vojvodina (purified and conditioned water) but in some sub-artesian wells there were HCH and aldrin residues. OCI residues were quantified in surface water of the river Danube which is in the required group, except for HCH in 1990/91, when it was in the III-IV group. Air was analyzed in Novi Sad, Zrenjanin, Kikinda, Beocin and Apatin. OCI residues are regularly tested in Zrenjanin (1996-2000): the most frequently detected are: HCH residues (37.83% samples), lindane (21.72% samples), heptachlor (17.60% samples) and rarely DTD and aldrin; aldrin (in traces), heptachlor and HCH were detected in Novi Sad. Heptachlor, lindane and HCH were also rarely determined in Kikinda. OCI have an affinity for fat tissues and their residues can be found particularly in foodstuffs originating from animals. They are not eliminated by thermal processing and it was established in pre-school institutions, student restaurants and homes for the aging; most frequently detected was p,p?DDE isomer, rarely p,p?DDT as well as HCH and rarely lindane. Although use of OCI is forbidden, due to their long persistence in the environment, they can be found in human tissues, which was proved by human serum analyses. Analyses of human autopsy material indicated presence of DDT metabolite - p,p?DDE as well as lindane in all analyzed samples (brain, liver, fat and tumorous tissues).

Download Full-text