Identification of Gracilariaceae (Rhodophyta) of central Portugal by histological and genetic methods

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Marta V. Freitas ◽  
Marco Simões ◽  
Christian Valdez ◽  
Clélia Afonso ◽  
Beatriz G. Trindade ◽  
...  
Keyword(s):  
Species Level ◽  
Its Sequencing ◽  
Species Identity ◽  
Gracilaria Gracilis ◽  
Future Studies ◽  
Cytochrome Oxidase I Gene ◽  
Central Portugal ◽  
Different Populations ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene

Abstract This study aims to identify different populations of Gracilariaceae collected from the central coast of Portugal through light microscopy, anatomical observations and genetic tools, essential approaches to correctly assign species identity. Samples were obtained from Ria de Aveiro (AV), Figueira da Foz (FFBC, FFMD), and Lagoa de Óbidos (LOBR, LOEV, LOBS). Although histological observations offered a visual representation of the characteristic pseudoparenchymatous organization, they did not allow a clear distinction among the species. The amplification of a ∼700 bp fragment of the mitochondrial cytochrome oxidase I gene, and its sequencing enabled us to assign the populations FFBC and LOBS to Gracilaria gracilis, and the populations AV, FFMD, LOBR, and LOEV to Agarophyton vermiculophyllum. This contribution will help phycologists to correctly identify the Portuguese populations of Gracilaria sensu lato at the species level, which will be crucial in ensuring that future studies and industrial exploration accurately target the correct species.

scholarly journals Fishes collected during the 2017 MarineGEO assessment of Kāne‘ohe Bay, O‘ahu, Hawai‘i

2020 ◽  
Vol 100 (4) ◽  
pp. 607-637
Author(s):  
Lynne R. Parenti ◽  
Diane E. Pitassy ◽  
Zeehan Jaafar ◽  
Kirill Vinnikov ◽  
Niamh E. Redmond ◽  
...  
Keyword(s):  
Smithsonian Institution ◽  
Genetic Distances ◽  
Taxonomic Identification ◽  
Tissue Samples ◽  
Long Term Storage ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
Term Storage ◽  
I Gene

AbstractWe report the results of a survey of the fishes of Kāne‘ohe Bay, O‘ahu, conducted in 2017 as part of the Smithsonian Institution MarineGEO Hawaii bioassessment. We recorded 109 species in 43 families. The most speciose families were Acanthuridae (11 species), Gobiidae (11 species), Pomacentridae (10) and Chaetodontidae (9 species). Nine of the species that we collected are known or suspected to be introduced to the Hawaiian Islands. Specimens were identified, measured and photographed. All specimen vouchers were fixed in formalin and ultimately transferred to 75% ethanol for long-term storage. For nearly all species, we took multiple tissue samples from specimen vouchers prior to formalin-fixation; we preserved tissues in 95% ethanol and then stored them at −80°C. The 5′-end of the mitochondrial cytochrome oxidase I gene (mtCOI) was sequenced for 94 species to confirm their taxonomic identification. Using these barcode sequences, we also measured genetic distances between collected individuals and their conspecifics from other localities outside Hawaii to verify the hypothesis that Hawaiian populations of species broadly distributed throughout the Indo-Pacific may be genetically distinct. We present select case studies to demonstrate the potential for undiscovered endemism in the Hawaiian fish biota.

Molecular analysis of mitochondrial cytochrome oxidase I gene of Aedes aegypti L. mosquitoes

2020 ◽  
Vol 23 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Ratnapal Gandhi ◽  
Kamlesh K. Yadav ◽  
Prabhakargouda B. Patil ◽  
Pankaj Bihani ◽  
Bharat Char ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Cytochrome Oxidase ◽  
Molecular Analysis ◽  
Cytochrome Oxidase I ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene

The Comparative Molecular Study between Bombycidae and Saturniidae Based on mtDNA RFLP and Cytochrome Oxidase I Gene Sequences: Implication for Molecular Evolution

1999 ◽  
Vol 54 (7-8) ◽  
pp. 587-594 ◽  
Author(s):  
Jae-Sam Hwang ◽  
Jin-Sung Lee ◽  
Tae-Won Goo ◽  
Hyun-Ah Kang ◽  
Hae-Ryong Sohn ◽  
...  
Keyword(s):  
Cytochrome Oxidase ◽  
Molecular Study ◽  
Cytochrome Oxidase I ◽  
Mitochondrial Cytochrome ◽  
Confidence Limits ◽  
Sequence Identity ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene ◽  
Mtdna Rflp

Abstract Bombycidae, Saturniidae, mtDNA RFLP, Cytochrome Oxidase I Gene The phylogenetic relationships between Bombyx mori and Bombyx mandarina species of Bombycidae, and Antheraea yamamai and Antheraea pernyi species of Saturniidae were investigated based on mtDNA RFLP and cytochrome oxidase I gene. The sizes of the mtDNA of all the species were estimated at approximately 16 kbp ± 500 bp by total length of all the restricted fragments and no variation in size was recognized. Of the fourteen different restriction endonucleases used, BamHl, Hindlll, Pstl, EcoRl and Xbal showed RFLP. Among these, only Hindlll showed RFLP between B. mori and B. mandarina. A comparative analysis of sequences was also conducted with the mitochondrial cytochrome oxidase I genes of each species. The results indicated that B. mori shared a 97% , 85% and 87% sequence identity with B. mandarina, A. yamamai and A. pernyi, respectively. B. mandarina shared a 87% and 88% sequence identity with A. yamamai and A. penyi, respectively. A. yamamai shared 92% sequence identity with A. pernyi. The results of the phylogenetic analysis exhib­ited monophyly and confidence limits of more than 99% in all trees for both Bombycidae and Saturniidae.

Description of the larvae of Drusus ramae Marinković-Gospodnetić and Drusus medianus Marinković-Gospodnetić (Trichoptera: Limnephilidae) with some genetic, distributional, ecological, faunal and conservation notes

Zootaxa ◽  
2010 ◽  
Vol 2484 (1) ◽  
pp. 1 ◽  
Author(s):  
MLADEN KUČINIĆ ◽  
ANA PREVIŠIĆ ◽  
SVJETLANA STANIĆ-KOŠTROMAN ◽  
MILIVOJ FRANJEVIĆ ◽  
LUCIJA ŠERIĆ JELASKA ◽  
...  
Keyword(s):  
Balkan Peninsula ◽  
The Other ◽  
Mitochondrial Cytochrome ◽  
Diagnostic Features ◽  
Conservation Implications ◽  
Cytochrome Oxidase I Gene ◽  
Gene Coi ◽  
Distribution And Ecology ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene

In this study we present morphological features of the last instar larvae of Drusus ramae Marinković-Gospodnetić, 1971, and Drusus medianus Marinković-Gospodnetić, 1976, both of which are endemic species from Bosnia and Herzegovina. Using the mitochondrial cytochrome oxidase I gene (COI), larvae of these species were successfully associated with adult specimens. We present the most important diagnostic features enabling separation from larvae of the other Drusus species and an identification key for the known larvae of Drusus species in the Balkan Peninsula. Notes on the distribution and ecology of both species are given. Additionally, faunistics and possible conservation implications for studied springs are discussed.

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