Occurrence of Aujeszky’s disease virus single-reactor pigs in Poland

AbstractThe aim of the study was to analyse the prevalence of single-reactor (SR) pigs in Poland, to attempt an explanation for this phenomenon, and to assess whether the occurrence of SR pigs could create problems for a successful Aujeszky’s disease virus (ADV) eradication programme in Poland. A total of 6494 ADV gE antibody positive/doubtful sera were retested by gB ELISA and subsequently by virus neutralisation test (VNT) to confirm the results of the glycoprotein E (gE) ELISA. Amongst the serum samples tested, 5.9% could be classified as being taken from SR pigs, as was shown by gE ELISA positive/doubtful results, which were not confirmed by negative findings in gB ELISA and VNT. It means that the observed SR phenomenon was due to a false positive/doubtful reaction in gE ELISA. This finding was strongly supported by the fact that the serum samples were taken from the animals from herds without any previous or subsequent history of Aujeszky’s disease. The low percentage of SR pigs does not seem to create a big obstacle to a successful ADV eradication programme in Poland.

Download Full-text

Isolation of mutants of Aujeszky’s disease virus with antigenically altered glycoprotein E by affinity chromatography using monoclonal antibodies

Journal of Virological Methods ◽

10.1016/s0166-0934(98)00137-2 ◽

1999 ◽

Vol 77 (1) ◽

pp. 101-108 ◽

Cited By ~ 2

Author(s):

O.S. Morenkov ◽

S.V. Smirnov ◽

V.V. Vrublevskaya

Keyword(s):

Monoclonal Antibodies ◽

Affinity Chromatography ◽

Disease Virus ◽

Glycoprotein E ◽

Aujeszky's Disease ◽

Aujeszky’S Disease ◽

Aujeszky’S Disease Virus

Download Full-text

Prevalence of antibodies to Aujeszky’s disease virus in wild boar in Poland, between 2011 and 2014: a retrospective study

Journal of Veterinary Research ◽

10.1515/jvetres-2017-0052 ◽

2017 ◽

Vol 61 (4) ◽

pp. 397-404 ◽

Cited By ~ 1

Author(s):

Andrzej Lipowski ◽

Anna Szczotka-Bochniarz ◽

Zygmunt Pejsak

Keyword(s):

Wild Boar ◽

Disease Virus ◽

Special Importance ◽

Wild Boar Population ◽

Eradication Programme ◽

Domestic Pigs ◽

Wide Range ◽

Aujeszky's Disease ◽

Aujeszky’S Disease ◽

Aujeszky’S Disease Virus

AbstractIntroduction: Aujeszky’s disease virus (ADV) infects a wide range of animals, including members of the Suidae family, i.e. domestic and wild pigs, as well as wild boar. Since wild boar are a potential ADV reservoir and a source of infection for domestic pigs, the aim of the study was to evaluate ADV antibody prevalence in the Polish wild boar population, during the years 2011 to 2014.Material and Methods: Wild boar blood samples were collected during three consecutive hunting seasons; i.e. 2011/2012, 2012/2013, and 2013/2014, and tested for ADV antibodies by ELISA.Results: ADV antibodies were detected in samples from all tested voivodships. The average seroprevalence reached 32.2%. Seroprevalence, over the examined hunting seasons, was 27.4% in 2011/2012, 32.4% in 2012/2013, and 35.5% in 2013/2014. The highest percentage of seroreagents was detected in four voivodships, situated along the western border of Poland, i.e. Zachodnio-Pomorskie (ZP), Lubuskie (LB), Dolnośląskie (DS), and Opolskie (OP). This area is positively correlated with the highest density of the wild boar population and the highest wild boar hunting bag.Conclusion: The results of this study confirm that the wild boar population may still pose a threat to domestic pigs, which is of special importance at the final stage of Aujeszky’s disease eradication programme in Poland.

Download Full-text

Immunological characterisation of glycoprotein E of Aujeszky's disease virus

Virus Research ◽

10.1016/s0168-1702(97)00077-4 ◽

1997 ◽

Vol 51 (1) ◽

pp. 65-79 ◽

Cited By ~ 9

Author(s):

Oleg S. Morenkov ◽

Nadja Fodor ◽

Yuri A. Sobko ◽

Istvan Fodor

Keyword(s):

Disease Virus ◽

Glycoprotein E ◽

Aujeszky's Disease ◽

Aujeszky’S Disease ◽

Aujeszky’S Disease Virus

Download Full-text

Indirect Elisas Based on Recombinant and Affinity-Purified Glycoprotein E of Aujeszky's Disease Virus to Differentiate Between Vaccinated and Infected Animals

Acta Veterinaria Hungarica ◽

10.1556/avet.47.1999.1.15 ◽

1999 ◽

Vol 47 (1) ◽

pp. 137-150 ◽

Cited By ~ 4

Author(s):

O. S. Morenkov ◽

Nadja Fodor ◽

I. Fodor

Keyword(s):

Recombinant Protein ◽

Disease Virus ◽

Test Performance ◽

Maximum Level ◽

Glycoprotein E ◽

Specificity And Sensitivity ◽

Aujeszky's Disease ◽

Aujeszky’S Disease ◽

Aujeszky’S Disease Virus ◽

High Dilutions

Two indirect ELISAs for the detection of antibodies against glycoprotein E (gE) of Aujeszky's disease virus (ADV) in sera have been developed. The rec-gE-ELISA is based on theE. coli-expressed recombinant protein containing the N-terminal sequences of gE (aa 1-125) fused with the glutathione S-transferase fromSchistosoma japonicum. The affi-gE-ELISA is based on native gE, which was purified from virions by affinity chromatography. The tests were optimised and compared with each other, as well as with the recently developed blocking gE-ELISA (Morenkov et al., 1997b), with respect to specificity and sensitivity. The rec-gE-ELISA was less sensitive in detecting ADV-infected animals than the affi-gE-ELISA (sensitivity 80% and 97%, respectively), which is probably due to the lack of conformation-dependent immunodominant epitopes on the recombinant protein expressed inE. coli. The specificity of the rec-gE-ELISA and affi-gE-ELISA was rather moderate (90% and 94%, respectively) because it was necessary to set such cut-off values in the tests that provided a maximum level of sensitivity, which obviously increased the incidence of false positive reactions. Though the indirect ELISAs detect antibodies against many epitopes of gE, the blocking gE-ELISA, which detects antibodies against only one immunodominant epitope of gE, showed a better test performance (specificity 99% and sensitivity 98%). This is most probably due to rather high dilutions of the sera used in the indirect gE-ELISAs (1:30) as compared to the serum dilution in the blocking gE-ELISA (1:2). We conclude that the indirect gE-ELISAs are sufficiently specific and sensitive to distinguish ADV-infected swine from those vaccinated with gE-negative vaccine and can be useful, in particularly affi-gE-ELISA, as additional tests for the detection of antibodies to gE.

Download Full-text