Characterization of three new plasmids from Lactobacillus paracasei 54

2015 ◽  
Vol 40 (3) ◽  
Author(s):  
Xuan Zhu ◽  
Yizhen Zhao ◽  
Chen Zhang ◽  
Lei Shen ◽  
Han Jiang ◽  
...  
Keyword(s):  
Newborn Infant ◽  
Food Industry ◽  
Lactobacillus Paracasei ◽  
Rolling Circle Replication ◽  
Fecal Samples ◽  
Rolling Circle ◽  
Healthy Newborn ◽  
Roche 454

AbstractObjective: Three plasmids from Lactobacillus paracasei 54 were isolated from healthy newborn infant fecal samples.Methods: Plasmid was extracted using an AxyPrep Plasmid Miniprep Kit and lysozyme. The extracted plasmids were sequenced using a Roche 454 Genome Sequencer FLX.Results: Three plasmids were isolated from Lactobacillus paracasei 54. These plasmids are designated pLP5401- 03, and they are 9754, 6650, and 1788 bp in size.Conclusion: Plasmids pLP5401 and pLP5402 were found to contain replication genes that are likely to function via the theta-type mechanism. Plasmid pLP5403 is predicted to replicate via the rolling-circle replication (RCR) mechanism. The RCR replication plasmid can be applied as a useful vector in the food industry

scholarly journals Characterization of pR18, a novel rolling-circle replication plasmid from Lactobacillus plantarum

Plasmid ◽  
2014 ◽  
Vol 73 ◽  
pp. 1-9 ◽  
Author(s):  
Tannaz Jalilsood ◽  
Ali Baradaran ◽  
Foo Hooi Ling ◽  
Shuhaimi Mustafa ◽  
Khatijah Yusof ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Rolling Circle Replication ◽  
Rolling Circle

Characterization of a Rolling-Circle Replication Plasmid pLR1 from Lactobacillus plantarum LR1

2008 ◽  
Vol 58 (2) ◽  
pp. 106-110 ◽  
Author(s):  
Ruoyu Li ◽  
Zhengyuan Zhai ◽  
Sheng Yin ◽  
Ying Huang ◽  
Qing Wang ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Rolling Circle Replication ◽  
Rolling Circle

Characterization of a novel rolling-circle replication plasmid pYSI8 from Lactobacillus sakei YSI8

Plasmid ◽  
2009 ◽  
Vol 62 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Zhengyuan Zhai ◽  
Yanling Hao ◽  
Sheng Yin ◽  
Chunguang Luan ◽  
Liebing Zhang ◽  
...  
Keyword(s):  
Lactobacillus Sakei ◽  
Rolling Circle Replication ◽  
Rolling Circle

scholarly journals Biochemical Characterization of theStaphylococcus aureusPcrA Helicase and Its Role in Plasmid Rolling Circle Replication

2002 ◽  
Vol 277 (48) ◽  
pp. 45880-45886 ◽  
Author(s):  
Tseh-Ling Chang ◽  
Asma Naqvi ◽  
Syam P. Anand ◽  
M. Gabriela Kramer ◽  
Rajan Munshi ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Rolling Circle Replication ◽  
Rolling Circle

scholarly journals Characterization of a rolling-circle replication plasmid from Thermus aquaticus NTU103

Plasmid ◽  
2006 ◽  
Vol 56 (1) ◽  
pp. 46-52 ◽  
Author(s):  
Sheng-Fen Chu ◽  
Hung-Yu Shu ◽  
Ling-Chun Lin ◽  
Mao-Yen Chen ◽  
San-San Tsay ◽  
...  
Keyword(s):  
Rolling Circle Replication ◽  
Thermus Aquaticus ◽  
Rolling Circle

Characterization of a rolling-circle replication plasmid pXY3 from Lactobacillus plantarum XY3

Plasmid ◽  
2010 ◽  
Vol 64 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Hui Zhou ◽  
Yanling Hao ◽  
Ying Xie ◽  
Sheng Yin ◽  
Zhengyuan Zhai ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Rolling Circle Replication ◽  
Rolling Circle

scholarly journals Characterization of a Staphylococcal Plasmid Related to pUB110 and Carrying Two Novel Genes, vatC andvgbB, Encoding Resistance to Streptogramins A and B and Similar Antibiotics

1998 ◽  
Vol 42 (7) ◽  
pp. 1794-1798 ◽  
Author(s):  
Jeanine Allignet ◽  
Nadia Liassine ◽  
Névine El Solh
Keyword(s):  
Antibiotic Resistance Genes ◽  
Direct Repeats ◽  
Rolling Circle Replication ◽  
Recombination Site ◽  
Site Specific ◽  
Rolling Circle ◽  
Gram Positive Bacteria ◽  
The Common ◽  
Staphylococcus Cohnii

ABSTRACT We isolated and sequenced a plasmid, named pIP1714 (4,978 bp), which specifies resistance to streptogramins A and B and the mixture of these compounds. pIP1714 was isolated from a Staphylococcus cohnii subsp. cohnii strain found in the environment of a hospital where pristinamycin was extensively used. Resistance to both compounds and related antibiotics is encoded by two novel, probably cotranscribed genes, (i) vatC, encoding a 212-amino-acid (aa) acetyltransferase that inactivates streptogramin A and that exhibits 58.2 to 69.8% aa identity with the Vat, VatB, and SatA proteins, and (ii) vgbB, encoding a 295-aa lactonase that inactivates streptogramin B and that shows 67% aa identity with the Vgb lactonase. pIP1714 includes a 2,985-bp fragment also found in two rolling-circle replication and mobilizable plasmids, pUB110 and pBC16, from gram-positive bacteria. In all three plasmids, the common fragment was delimited by two direct repeats of four nucleotides (GGGC) and included (i) putative genes closely related to repB, which encodes a replication protein, and topre(mob), which encodes a protein required for conjugative mobilization and site-specific recombination, and (ii) sequences very similar to the double- and single-strand origins (dso, ssoU ) and the recombination site, RSA. The antibiotic resistance genes repBand pre(mob) carried by each of these plasmids were found in the same transcriptional orientation.

Characterization of a Rolling-Circle Replication Plasmid pM411 from Lactobacillus plantarum 1–3

2016 ◽  
Vol 73 (6) ◽  
pp. 820-826 ◽  
Author(s):  
Xvchuan Ma ◽  
Jinhua Li ◽  
Yao Xiong ◽  
Zhengyuan Zhai ◽  
Fazheng Ren ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Rolling Circle Replication ◽  
Rolling Circle

Characterization of a Cryptic Rolling-Circle Replication Plasmid pMK8 from Enterococcus durans 1–8

2018 ◽  
Vol 75 (9) ◽  
pp. 1198-1205 ◽  
Author(s):  
Penyu Zhou ◽  
Zhengyuan Zhai ◽  
Xiong Yao ◽  
Peihua Ma ◽  
Yanling Hao
Keyword(s):  
Rolling Circle Replication ◽  
Rolling Circle ◽  
Enterococcus Durans
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