Effect of Cell Culture on 18S rRNA Gene Sequences in the Cultural Course of Taxus chinensis Cells

2008 ◽  
Vol 63 (1-2) ◽  
pp. 127-132
Author(s):  
Fu Xiang ◽  
Long J. Yu ◽  
Wu Chen ◽  
Zhi Liu
Keyword(s):  
Cell Culture ◽  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Relative Rate ◽  
18S Rrna Gene ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Taxus Chinensis ◽  
Gene Sequences ◽  
Parsimony Method

Cell culture is an effective technology for taxol production. This paper discusses the effect of Taxus cell cultures on the 18S rRNA gene sequences based on the phylogenetic analysis of cultured T. chinensis cells and related species. The phylogenetic tree is reconstructed using the maximum parsimony method and the relative rate test to test the hypothesis of a molecular clock. The phylogenetic analysis indicates that cell culture changes the phylogenetic position of cultured T. chinensis cells. More than that, the 18S rRNA gene of cultured T. chinensis cells has a faster rate of substitution than that of T. chinensis. With T. media as reference, the divergence time of the cultured T. chinensis cells is 7 Ma (million years) more than that of the T. chinensis cells based on the 18S rRNA gene sequences.

Phylogenetic Analysis Based on 18S rRNA Gene and matK Gene Sequences of Panax vietnamensis and Five Related Species

Planta Medica ◽  
2001 ◽  
Vol 67 (5) ◽  
pp. 461-465 ◽  
Author(s):  
Katsuko Komatsu ◽  
Shu Zhu ◽  
Hirotoshi Fushimi ◽  
Tran Kim Qui ◽  
Shaoqing Cai ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Related Species ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Gene Sequences ◽  
Matk Gene

Effect of DNA Methylation on 18S rRNA Gene Sequences during Culture of Taxus chinensis Cells

2009 ◽  
Vol 64 (5-6) ◽  
pp. 418-420
Author(s):  
Fu Xiang ◽  
Liqing Li ◽  
Wenwen Jin ◽  
Longjiang Yu
Keyword(s):  
Dna Methylation ◽  
18S Rrna ◽  
Gene Sequence ◽  
Gc Content ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Taxus Chinensis ◽  
Rrna Gene Sequence ◽  
Gene Sequences ◽  
New Variety

Cell suspension culture has rapidly become an alternative source of taxol, an anticancer compound. To investigate the role of DNA methylation in the cultural course of Taxus chinensis cells, analyses of 18S rRNA gene sequences of cultured T. chinensis cells and related species were conducted. The phylogenetic analysis of 18S rRNA gene sequences indicated that HG-1 (the cultured T. chinensis cells), like T. mairei (the natural variety of T. chinensis), should be a new variety of T. chinensis, and cell culture can change the 18S rRNA gene sequence at the level of species despite 18S rRNA is the most conserved gene. The analyses of the CpG and TpG+CpA relative abundance and GC content of the 18S rRNA gene sequences made clear that DNA methylation contributed to changes of the 18S rRNA gene sequence of HG-1 at the level of species, which can make HG-1 to become a new variety of T. chinensis.

scholarly journals Phylogenetic Analysis of Indian Freshwater Pond Mussels Lamellidens corrianus and L. phenchooganjensis (Bivalvia: Unionidae) from the Upper Brahmaputra Basin of Assam, India

2021 ◽  
Vol 18 (1) ◽  
pp. 197-206
Author(s):  
Jyotish Sonowal ◽  
Pankaj Chetia ◽  
Devid Kardong
Keyword(s):  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Gene Sequence ◽  
18S Rrna Gene ◽  
Phylogenetic Study ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequences ◽  
Freshwater Molluscs ◽  
First Time

To update the present knowledge of freshwater molluscs, a phylogenetic analysis of two species of Indian pond mussels was carried out using amplified sequence of 18S rRNA gene. From the phylogenetic study of 18S rRNA gene sequencing, it was found that the Lamellidenscorrianus and L. phenchooganjensis are phylogenetically closely related to 18S rRNA gene sequences of other freshwater bivalve mussels belonging to the family Unionidae of order Unionida. Like other members of the Unionidae family, the two Lamellidensspp. showed monophyletic evolutionary lineage and shared a common ancestor. The result obtained from the phylogenetic analysis of Lamellidensspp. was significant as the 18S rRNA gene sequence of L. phenchooganjensis was submitted for the first time in the global nucleotide database (Genbank, NCBI). Similarly, the 18S rRNA gene sequence of L. corrianuswas also submitted to the database for the first time from this region having unique ecological niche. Therefore, the present study on phylogenetic analysis is a contribution to the global database of 18S rRNA gene sequences of freshwater mollusc, particularly from this part of the region.

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