scholarly journals The Core Membrane Fusion Complex Governs the Probability of Synaptic Vesicle Fusion But Not Transmitter Release Kinetics

2002 ◽  
Vol 22 (4) ◽  
pp. 1266-1272 ◽  
Author(s):  
Michael F. A. Finley ◽  
Sejal M. Patel ◽  
Daniel V. Madison ◽  
Richard H. Scheller
Keyword(s):  
Membrane Fusion ◽  
Synaptic Vesicle ◽  
Transmitter Release ◽  
Release Kinetics ◽  
Vesicle Fusion ◽  
The Core ◽  
Synaptic Vesicle Fusion

scholarly journals Post-tetanic potentiation lowers the energy barrier for synaptic vesicle fusion independently of Synaptotagmin-1

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Vincent Huson ◽  
Marieke Meijer ◽  
Rien Dekker ◽  
Mirelle ter Veer ◽  
Marvin Ruiter ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Energy Barrier ◽  
Phorbol Esters ◽  
Release Kinetics ◽  
Vesicle Fusion ◽  
Tetanic Stimulation ◽  
Release Rates ◽  
Synaptotagmin 1 ◽  
Post Tetanic Potentiation ◽  
Synaptic Vesicle Fusion

Previously, we showed that modulation of the energy barrier for synaptic vesicle fusion boosts release rates supralinearly (Schotten, 2015). Here we show that mouse hippocampal synapses employ this principle to trigger Ca2+-dependent vesicle release and post-tetanic potentiation (PTP). We assess energy barrier changes by fitting release kinetics in response to hypertonic sucrose. Mimicking activation of the C2A domain of the Ca2+-sensor Synaptotagmin-1 (Syt1), by adding a positive charge (Syt1D232N) or increasing its hydrophobicity (Syt14W), lowers the energy barrier. Removing Syt1 or impairing its release inhibitory function (Syt19Pro) increases spontaneous release without affecting the fusion barrier. Both phorbol esters and tetanic stimulation potentiate synaptic strength, and lower the energy barrier equally well in the presence and absence of Syt1. We propose a model where tetanic stimulation activates Syt1-independent mechanisms that lower the energy barrier and act additively with Syt1-dependent mechanisms to produce PTP by exerting multiplicative effects on release rates.

scholarly journals Post-tetanic potentiation lowers the energy barrier for synaptic vesicle fusion independently of Synaptotagmin-1

2020 ◽  
Author(s):  
Vincent Huson ◽  
Marieke Meijer ◽  
Rien Dekker ◽  
Mirelle ter Veer ◽  
Marvin Ruiter ◽  
...  
Keyword(s):  
Synaptic Vesicle ◽  
Energy Barrier ◽  
Phorbol Esters ◽  
Release Kinetics ◽  
Vesicle Fusion ◽  
Tetanic Stimulation ◽  
Release Rates ◽  
Synaptotagmin 1 ◽  
Post Tetanic Potentiation ◽  
Synaptic Vesicle Fusion

AbstractPreviously, we showed that modulation of the energy barrier for synaptic vesicle fusion boosts release rates supralinearly (Schotten, 2015). Here we show that mouse hippocampal synapses employ this principle to trigger Ca2+-dependent vesicle release and post-tetanic potentiation (PTP). We assess energy barrier changes by fitting release kinetics in response to hypertonic sucrose. Mimicking activation of the C2A domain of the Ca2+-sensor Synaptotagmin-1 (Syt1), by adding a positive charge (Syt1D232N) or increasing its hydrophobicity (Syt14W), lowers the energy barrier. Removing Syt1 or impairing its release inhibitory function (Syt19Pro) increases spontaneous release without affecting the fusion barrier. Both phorbol esters and tetanic stimulation potentiate synaptic strength, and lower the energy barrier equally well in the presence and absence of Syt1. We propose a model where tetanic stimulation activates Syt1 dependent and independent mechanisms that lower the energy barrier independently in an additive manner to produce PTP by multiplication of release rates.

scholarly journals Distinct domains of syntaxin are required for synaptic vesicle fusion complex formation and dissociation

Neuron ◽  
1995 ◽  
Vol 14 (5) ◽  
pp. 991-998 ◽  
Author(s):  
Yun Kee ◽  
Richard C Lin ◽  
Shu-Chan Hsu ◽  
Richard H Scheller
Keyword(s):  
Complex Formation ◽  
Synaptic Vesicle ◽  
Vesicle Fusion ◽  
Synaptic Vesicle Fusion

scholarly journals A Novel Synaptic Vesicle Fusion Path in the Rat Cerebral Cortex: The “Saddle” Point Hypothesis

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100710 ◽  
Author(s):  
Guido A. Zampighi ◽  
Raul Serrano ◽  
Julio L. Vergara
Keyword(s):  
Cerebral Cortex ◽  
Saddle Point ◽  
Synaptic Vesicle ◽  
Vesicle Fusion ◽  
Rat Cerebral Cortex ◽  
Synaptic Vesicle Fusion

scholarly journals Palmitoylation of the synaptic vesicle fusion machinery

2009 ◽  
Vol 110 (4) ◽  
pp. 1135-1149 ◽  
Author(s):  
Gerald R. Prescott ◽  
Oforiwa A. Gorleku ◽  
Jennifer Greaves ◽  
Luke H. Chamberlain
Keyword(s):  
Synaptic Vesicle ◽  
Vesicle Fusion ◽  
Synaptic Vesicle Fusion
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