scholarly journals Seed genetic purity testing of F1 Benincasa hispida var. chieh-qua hybrids using SSR molecular marker analysis

2020 ◽  
Vol 48 (3) ◽  
pp. 345-353
Author(s):  
J.Y. Chen ◽  
Q.M. Chen ◽  
Z.G. Liu ◽  
C.L. Wang ◽  
L.L. Ma ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Seed Quality ◽  
Accurate Method ◽  
Whole Genome Sequence ◽  
Genetic Purity ◽  
Purity Testing ◽  
Benincasa Hispida ◽  
Ssr Primers ◽  
Hybrid Seeds ◽  
Molecular Marker Analysis

To ensure that farmers can access high-quality seeds, it is essential to find a simple, rapid and accurate method to assess seed purity. In recent years, heterosis in chieh-qua has been widely applied to production. Using the whole genome sequence of chieh-qua, we designed simple sequence repeat (SSR) primers specific for chieh-qua. The parental lines of nine hybrids were screened using 200 SSR primers, seven of which exhibited polymorphisms. The bands were clear, stable and reproducible. We found dominant and co-dominant bands between the parents of the nine hybrids. The seven pairs of SSR primers were successfully used to assess genetic purity of the nine chieh-qua hybrids. The SSR molecular marker purity assessment results were consistent with the results obtained from a field grow-out test (GOT). However, the use of SSR markers provided a more accurate, reliable and faster method for seed purity testing than the GOT. We propose using SSR molecular marker technology to assess the genetic purity of chieh-qua hybrid seeds. With this method, the seed quality can be determined faster, which may help to accelerate the chieh-qua breeding process.

scholarly journals Use of molecular marker techniques in seed testing by Brazilian seed companies

1998 ◽  
Vol 55 (spe) ◽  
pp. 79-82 ◽  
Author(s):  
P.T. Della Vecchia ◽  
C.A.R. da Silva ◽  
P. Terenciano-Sobrinho
Keyword(s):  
Molecular Marker ◽  
New Technologies ◽  
Seed Quality ◽  
Genetic Purity ◽  
Process Step ◽  
Seed Testing ◽  
Routine Basis ◽  
Seed Market ◽  
Key Issues ◽  
High Quality Seed

Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD, microsatelites, AFLP) for genetic purity determinations in seed testing. As far as we know, no Brazilian seed company is using, on regular basis, RAPD or other molecular marker techniques in the determination of genetic purity in seed testing. Most of these are using morphological or physiological traits expressed by seed, seedling or mature plant and/or electrophoresis of seed or seedling proteins/isoenzymes for that purpose. Main reasons for that are: DNA molecular marker techniques are relatively new; lack of specialized personnel to run DNA molecular marker assays on routine basis; higher cost/sample when compared to proteins/isoenzymes electrophoresis.

GENETIC PURITY TEST OF F1 HYBRID TOMATO USING MOLECULAR MARKER ANALYSIS

2008 ◽  
pp. 231-238 ◽  
Author(s):  
L. Liu ◽  
Y. Wang ◽  
Y. Gong ◽  
X. Zhai ◽  
F. Yu ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Marker Analysis ◽  
Purity Test ◽  
Molecular Marker Analysis

scholarly journals DNA fingerprinting of foxtail millet (Setaria italica L.) variety ATL 1 using SSR and RAPD markers along with morphological descriptors

2020 ◽  
Vol 7 (3) ◽  
pp. 587-593
Author(s):  
Senthil Natesan ◽  
◽  
Gowtham Murugesan ◽  
Nandhini Murugan ◽  
Sarankumar Chandran ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Rapd Markers ◽  
Plant Genetic Resources ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
National Bureau ◽  
Genetic Purity ◽  
Purity Testing ◽  
Ssr Primers

Foxtail millet (Setaria italica) is a cultivated nutritional cereal, which originated in South Asia and is considered one of the oldest cultivated millets in India. DNA fingerprinting is mandatory for registration of newly developed varieties with National Bureau of Plant Genetic Resources (NBPGR) and Protection of Plant Varieties and Farmers' Rights Authority (PPV&FRA). Due to the limited availability of genomic information in foxtail millet, the use of DNA based markers in fingerprinting of crop varieties is also limited. Hence in the present investigation, available RAPD and SSR markers of cereals are used for fingerprinting the foxtail millet varieties. The newly released variety ATL 1 is differentiated from popular variety CO (Te) 7 using SSR and RAPD markers. About 66 maize SSR primers, 16 sorghum SSR primers, and 10 RAPD primers were used in the study. Out of 66 maize SSR markers used for study, one showed polymorphism. The marker umc1704 showed polymorphism between CO (Te) 7 and ATL 1 by the presence of 670 bp allele CO (Te) 7. The RAPD primers OPB4, OPA5, OPA11 and OPB1 also helped for differentiation of the two varieties. The identified makers will help for genetic purity testing of CO (Te) 7 and ATL 1 in the seed chain.

Genetic purity and testing technologies for seed quality: a company perspective

1998 ◽  
Vol 8 (2) ◽  
pp. 285-294 ◽  
Author(s):  
J. S. C. Smith ◽  
J. C. Register III
Keyword(s):  
Single Molecule ◽  
Seed Quality ◽  
Genetic Purity ◽  
Biochemical Methods ◽  
Crop Varieties ◽  
Select Group ◽  
Purity Testing ◽  
Length Polymorphisms ◽  
Micro Array ◽  
High Level

AbstractA high level of genetic purity in crop varieties must be achieved and maintained for agronomic performance as well as to encourage investment and innovation in plant breeding and to ensure that the improvements in productivity and quality imparted by breeders are delivered to the farmer and, ultimately, to the consumer. Traditionally, morphological comparisons have formed the basis for genetic purity evaluations. However, replicated field observations are time-consuming, expensive and unreliable. Morphology cannot provide information on the purity of specific genetic attributes that relate to grain quality or to pest or herbicide resistance bred into varieties. Biochemical assays, including isozymes, can distinguish varieties within several species. Isozymes have been routinely used in checking seed-lot purity in maize (Zea mays L.) for the past 20 years. Newer DNA-based technologies such as restriction fragment length polymorphisms and more recently developed methods that use the polymerase chain reaction can allow even more discriminative and faster identification of varieties. However, none of the DNA methods have replaced biochemical methods for seed purity assays, other than in a relatively select group of crops with very high seed value, due to their high datapoint cost. It will require further miniaturization, automation and enhanced capabilities to process numerous samples simultaneously before newly developed methods supplant biochemical methods for routine usage in purity testing. New varieties that have major genes for herbicide or insect resistance incorporated within them require purity assays during product development and following seed production of the commercial variety. Immunological or DNA sequence assays can be developed and automated systems are required to process hundreds of thousands of individuals. Ultra-high, micro-array technologies and single-molecule detection systems are now under development. These technologies offer the promise that adequate distinction and high sample throughput will be combined. New methods may eclipse the capabilities of biochemical methodologies, thereby potentially raising genetic purity standards and enabling farmers and consumers better to utilize and benefit from increasingly productive varieties that are bred from a more diverse base of genetic resources.

scholarly journals Coincidence of flowering time and the productivity and quality of cauliflower hybrid seeds

2001 ◽  
Vol 58 (3) ◽  
pp. 533-539
Author(s):  
Marcelo Fontanetti Verdial ◽  
Antonio Ismael Inácio Cardoso ◽  
Márcio Santos de Lima ◽  
Francisco Célio Maia Chaves
Keyword(s):  
Flowering Time ◽  
Seed Quality ◽  
Standard Test ◽  
Genetic Purity ◽  
Plant Weight ◽  
Flowering Season ◽  
Sowing Dates ◽  
Hybrid Seeds ◽  
Number Of Seeds

The missing of flowering synchronization between the self-incompatible lines in a crop field of cauliflower hybrid seeds besides making the seed production smaller can compromise the genetic purity of them. The coincidence of the flowering time between two cauliflower lines was examined to study its effect on the productivity and quality of hybrid seeds. The treatments consisted of six different sowing dates, every fifteen days, using a self-incompatible tropical line pollinated by a winter line which does not present self-incompatibility. The following characteristics were evaluated: leaf average area and number of flowers per plant, number of siliques per plant, number and weight of seeds per plant, weight of thousand seeds and average number of seeds per silique. The germination standard test and genetic seed purity were determined for each treatment. The coincident flowering season between cauliflower lines affects directly the productivity and the genetic quality of the produced hybrid seeds. The closer the flowering time coincidence between the lines, the greater the number of seeds per silique and the smaller the percentage of non-hybrid seedlings. However, the coincidence of the flowering season between lines was found to influence physiological seed quality.

scholarly journals Evaluation of Genetic Purity of F1 Hybrid Seeds in Cabbage with RAPD, ISSR, SRAP, and SSR Markers

HortScience ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 724-727 ◽  
Author(s):  
Liwang Liu ◽  
Guang Liu ◽  
Yiqin Gong ◽  
Wenhao Dai ◽  
Yan Wang ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Female Parent ◽  
Sequence Repeat ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Ssr Primers ◽  
Hybrid Seeds ◽  
Issr Primers

Four molecular marker systems—RAPD (random amplified polymorphic DNA), ISSR (intersimple sequence repeat), SRAP (sequence-related amplified polymorphism), and SSR (simple sequence repeat)—were used to evaluate seed genetic purity of a hybrid cabbage cultivar ‘Zaoxia 16’. Genetic relationships of the F1 hybrids and their parents were analyzed with 157 RAPD primers, 54 ISSR primers, 84 SRAP primer combinations, and 44 SSR primers. Three RAPD primers (NAURP2006, NAURP2020, and NAURP2031), two ISSR primers (NAUISR1058 and NAUISR1062), one SRAP primer combination (NAUSR04/NAURS05), and two SSR primers (NAUSSR1011 and NAUSSR1031), which produced male and female parent-specific markers simultaneously, were selected for testing the genetic purity of the F1 seeds. A total of 210 ‘Zaoxia 16’ hybrid individuals were investigated with these eight selected primers. Of these, 12 appeared to be false hybrids. Nine of the 12 putative false hybrids, confirmed with all eight primers, exhibited similar banding patterns to the female parent, suggesting that they could be derived from selfing of the female parent. The results were in accordance with those from field evaluations. This study showed that RAPD, ISSR, SRAP, and SSR markers are highly efficient and reproducible for genetic purity testing of cabbage commercial hybrid seeds.

scholarly journals Genetic purity testing of pumpkin hybrid seed by ultrathin-layer isoelectric focusing

2013 ◽  
Vol 13 (3) ◽  
pp. 200-202
Author(s):  
Min Yan
Keyword(s):  
Isoelectric Focusing ◽  
Seed Proteins ◽  
Parental Line ◽  
Genetic Purity ◽  
Male And Female ◽  
Purity Testing ◽  
Marker Band ◽  
Hybrid Seeds ◽  
Ph Range ◽  
Ultrathin Layer

Ultrathin-layer isoelectric focusing of seed proteins was used to test the genetic purity of pumpkin hybrid seeds. In the pH range of 5-8, one male marker band and two female marker bands were found in the male and female parental line, respectively. Based on the male marker band and female marker bands, the genetic purity of F1 seeds can easily be determined in the laboratory.

scholarly journals Sequence-characterized amplified region (SCAR) technique used for variety discrimination in vinca (Catharanthus roseus (L.) G.Don)

2002 ◽  
Vol 24 (1) ◽  
pp. 299-305 ◽  
Author(s):  
Carlos C.E Menezes ◽  
Tara Vantoai ◽  
Miller B McDonald ◽  
Tocio Sediyama
Keyword(s):  
Catharanthus Roseus ◽  
Seed Quality ◽  
Sequence Information ◽  
Genetic Purity ◽  
Banding Patterns ◽  
Sequence Characterized Amplified Region ◽  
Purity Testing ◽  
Polymorphic Bands ◽  
Scar Primers

Sequence-Characterized Amplified Region (SCAR) appears as a useful technique for genetic purity testing and variety discrimination, applicable to species in which some other techniques have failed. In particular, this technique is very attractive with species in which RAPD results were not consistent. The RAPD polymorphic bands were cloned, sequenced and from the sequence information, primers pairs for normal PCR were developed. Since the probability of obtaining successful SCAR primers from RAPD polymorphic bands was about 50%, a larger number of RAPD polymorphic bands are needed to develop sufficient SCAR primers for varietal discrimination in vinca. In addition, the efficiency of the SCAR technique is strongly affected by the quality of DNA extracted from seeds. The SCAR banding patterns obtained from vinca seed were consistent and repeatable making the results reliable for genetic purity testing and variety discrimination. The SCAR technique is simple, fast, relatively inexpensive and allows the use of DNA extracted from dry seeds, which is very important in a seed-quality evaluating program

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