Application of ultrathin-layer isoelectric focusing for genetic purity testing of F1 hybrid seeds in napa cabbage

2013 ◽  
Vol 41 (1) ◽  
pp. 148-153
Author(s):  
M. Yan
Keyword(s):  
Isoelectric Focusing ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Purity Testing ◽  
Hybrid Seeds ◽  
Ultrathin Layer

scholarly journals Genetic purity testing of pumpkin hybrid seed by ultrathin-layer isoelectric focusing

2013 ◽  
Vol 13 (3) ◽  
pp. 200-202
Author(s):  
Min Yan
Keyword(s):  
Isoelectric Focusing ◽  
Seed Proteins ◽  
Parental Line ◽  
Genetic Purity ◽  
Male And Female ◽  
Purity Testing ◽  
Marker Band ◽  
Hybrid Seeds ◽  
Ph Range ◽  
Ultrathin Layer

Ultrathin-layer isoelectric focusing of seed proteins was used to test the genetic purity of pumpkin hybrid seeds. In the pH range of 5-8, one male marker band and two female marker bands were found in the male and female parental line, respectively. Based on the male marker band and female marker bands, the genetic purity of F1 seeds can easily be determined in the laboratory.

logo Genetic purity testing of two-line hybrid rice seeds by ultrathin-layer isoelectric focusing of proteins

2005 ◽  
Vol 33 (1) ◽  
pp. 45-52 ◽  
Author(s):  
T. Zhao ◽  
M. Yan ◽  
Y.P. Lu ◽  
F. Yang ◽  
J. Huang ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Hybrid Rice ◽  
Genetic Purity ◽  
Purity Testing ◽  
Rice Seeds ◽  
Ultrathin Layer

Genetic purity analysis of hybrid broccoli (Brassica oleracea var. italica) seeds using RAPD PCR

2002 ◽  
Vol 53 (1) ◽  
pp. 51 ◽  
Author(s):  
Phillip A. Crockett ◽  
Mohan B. Singh ◽  
C. K. Lee ◽  
Prem L Bhalla
Keyword(s):  
Plant Size ◽  
Vegetable Crops ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Rapd Pcr ◽  
Purity Analysis ◽  
Hybrid Seeds ◽  
Contrast Response

Determination of genetic purity of F1-hybrid seeds is aquality control requirement in the production of hybrid Brassica vegetableseeds. Hybrid varieties of these vegetable crops have arisen from a limitedgermplasm base, making discrimination of parental and hybrid lines verylaborious and troublesome. The use of RAPD PCR for evaluating seed purity in acommercial F1-hybrid broccoli a single cultivar bywithholding water. In Expt 2, plants of EP and MK were grown together in thesame container and received water daily with gradation in intensity of waterdeficit achieved by varying the daily water ration per container.All cultivars in each experiment exhibited commonly reported responses towater deficit, characterised by diminished evaporative surface area andincreased root : shoot ratio. The response of MK was primarily morphologicaland MK plants had smaller plant size, higher root : shoot ratio, and a lowergrowth rate compared with temperate cultivars. By contrast, response oftemperate cultivars was primarily physiological; stomatal conductance oftemperate cultivars was lower and these cultivars had a greater tendency forleaf lamina osmotic a

scholarly journals Seed genetic purity testing of F1 Benincasa hispida var. chieh-qua hybrids using SSR molecular marker analysis

2020 ◽  
Vol 48 (3) ◽  
pp. 345-353
Author(s):  
J.Y. Chen ◽  
Q.M. Chen ◽  
Z.G. Liu ◽  
C.L. Wang ◽  
L.L. Ma ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Seed Quality ◽  
Accurate Method ◽  
Whole Genome Sequence ◽  
Genetic Purity ◽  
Purity Testing ◽  
Benincasa Hispida ◽  
Ssr Primers ◽  
Hybrid Seeds ◽  
Molecular Marker Analysis

To ensure that farmers can access high-quality seeds, it is essential to find a simple, rapid and accurate method to assess seed purity. In recent years, heterosis in chieh-qua has been widely applied to production. Using the whole genome sequence of chieh-qua, we designed simple sequence repeat (SSR) primers specific for chieh-qua. The parental lines of nine hybrids were screened using 200 SSR primers, seven of which exhibited polymorphisms. The bands were clear, stable and reproducible. We found dominant and co-dominant bands between the parents of the nine hybrids. The seven pairs of SSR primers were successfully used to assess genetic purity of the nine chieh-qua hybrids. The SSR molecular marker purity assessment results were consistent with the results obtained from a field grow-out test (GOT). However, the use of SSR markers provided a more accurate, reliable and faster method for seed purity testing than the GOT. We propose using SSR molecular marker technology to assess the genetic purity of chieh-qua hybrid seeds. With this method, the seed quality can be determined faster, which may help to accelerate the chieh-qua breeding process.

Genetic purity testing of F1 hybrid seed with molecular markers in cabbage (Brassica oleracea var. capitata)

2013 ◽  
Vol 155 ◽  
pp. 92-96 ◽  
Author(s):  
Shan Ye ◽  
Yan Wang ◽  
Danqiong Huang ◽  
Jianbin Li ◽  
Yiqin Gong ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Oleracea ◽  
Hybrid Seed ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Purity Testing

scholarly journals Evaluation of Genetic Purity of F1 Hybrid Seeds in Cabbage with RAPD, ISSR, SRAP, and SSR Markers

HortScience ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 724-727 ◽  
Author(s):  
Liwang Liu ◽  
Guang Liu ◽  
Yiqin Gong ◽  
Wenhao Dai ◽  
Yan Wang ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Female Parent ◽  
Sequence Repeat ◽  
Genetic Purity ◽  
F1 Hybrid ◽  
Ssr Primers ◽  
Hybrid Seeds ◽  
Issr Primers

Four molecular marker systems—RAPD (random amplified polymorphic DNA), ISSR (intersimple sequence repeat), SRAP (sequence-related amplified polymorphism), and SSR (simple sequence repeat)—were used to evaluate seed genetic purity of a hybrid cabbage cultivar ‘Zaoxia 16’. Genetic relationships of the F1 hybrids and their parents were analyzed with 157 RAPD primers, 54 ISSR primers, 84 SRAP primer combinations, and 44 SSR primers. Three RAPD primers (NAURP2006, NAURP2020, and NAURP2031), two ISSR primers (NAUISR1058 and NAUISR1062), one SRAP primer combination (NAUSR04/NAURS05), and two SSR primers (NAUSSR1011 and NAUSSR1031), which produced male and female parent-specific markers simultaneously, were selected for testing the genetic purity of the F1 seeds. A total of 210 ‘Zaoxia 16’ hybrid individuals were investigated with these eight selected primers. Of these, 12 appeared to be false hybrids. Nine of the 12 putative false hybrids, confirmed with all eight primers, exhibited similar banding patterns to the female parent, suggesting that they could be derived from selfing of the female parent. The results were in accordance with those from field evaluations. This study showed that RAPD, ISSR, SRAP, and SSR markers are highly efficient and reproducible for genetic purity testing of cabbage commercial hybrid seeds.

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