Yield components of the M7 Kipas Putih mutant soybean

This study aimed to obtain the homogeny of M8 soybean lines in an effort to release new high yielding varieties. The research was conducted in Lamsidaya village, and the observation of yield components was carried out at the Plant Breeding Laboratory, Faculty of Agriculture, Universitas Syiah Kuala. This study did not use an experimental design, so that the quantitative characters were observed individually from each plant, then analyzed by estimating the heritability broad sense value and the progress of selection. The material used were var. Kipas Putih and var. Dega1 as control, eight of 7th generation Kipas Putih mutant lines (B4, B7, B10, B12, B13, B15, B18 and B22). All genotypes were planted in rectangular plot 10 m x 1 m, drainage 50 cm, and planting distance 50 cm x 30 cm. Plants were maintained in accordance with the recommendation for soybean cultivation from Balitkabi, and fertilizer NPK (16:16:16) was given 200 g/plot (200 kgha-1). Parameters observed included plant height, flowering time, harvest time, number of productive branches, total number of pods per plant, number of filled pods per plant, percentage of filled pods per plant, seed weight per plant, weight of 100 seeds, seed weight per plot, and yield potential. All quantitative characters indicated increase on selection except for the character of plant height, percentage of filled pods and weight of 100 seeds because the heritability value was low. The B18 line has the potential to become a new high yielding variety because it has a high yield potential (3.02 tons ha-1) with a large seed size compared to the other seven mutant lines and is also higher than the parent Kipas Putih and var Dega 1 varieties.

IDENTIFICATION OF SOME ROOTSTOCKS FOR WATERMELON CULTURES FROM ROMANIA

Among the grafting aims are (1) to enhance plant growth, fruit yield and quality; (2) to control wilt caused by pathogens; (3) to reduce viral, fungal and bacterial infection; (4) to strengthen tolerance to thermal or saline stress; (5) to increase nutrient and mineral uptake to the shoot. The cultivars used to obtain of grafted seedlings were from the Baronesa F1 (Citrullus lanatus) hybrid scion and the Pelops F1 (Lagenaria siceraria), Kiwano (Cucumis metuliferus) and Zefir (Benicasa hispida) rootstocks. The rootstock has influenced the number of fruits per plant, weight/fruit and production/plant; the Pelops rootstock has had a positive influence and the Kiwano and Zefir rootstocks have had a negative influence compared to the non-grafted variant. The Pelops rootstock (105.84 t/ha) has had a positive influence and the Kiwano rootstock (53.45 t/ha) and Zefir rootstock (51.38 t/ha) have had have a negative influence compared to the non-grafted variant (95.5 t/ha). The biometric measurements on watermelon fruit yield were made in 2020 year. The experience aimed the identification of some rootstocks for the watermelon cultures from Romania. The research shows that the rootstocks has influenced fruit yield and some grafting combinations researched may be recommended for cropping in Romania.

VARIATION OF SOME NEW MORPHOLOGICAL CHARACTERS IN THE FIELD PEAS (PISUM SATIVUM L.)

Peas, as a valuable nutritious and cultivated plant (Myers et al., 2010), have recently received special attention for the improvement of new varieties (Kreplak et al., 2019). They are increasingly adapted to any kind of environmental conditions. Thus, we want an increased production of grains, contents in active principles as high as possible (Pownall et al., 2010), but also to increase its proportion in the structure of crops on a farm. The Alvesta variety, studied for its specific morphological characters, is one of the newest creations. Even in the slightly drier conditions of the last period (two years), this variety formed plants with heights of 42-45 cm, with a total plant weight of 7.5 g. 10 knots were formed on a pea stem (at one node the floral and fruit raceme are caught and formed). Each plant formed 4 pods, weighing 6 g and 16 berries weighing 4.5-6 g. The bean had a diameter of 7 mm and the absolute weight of the berries was 215 g. Among these morphological characters were obtained significantly positive correlations in most cases. Insignificant situations were between the absolute mass of the berries with the size of the plant, with the number of nodes, with the total number of pods and with the number of berries on a plant. Only one insignificant negative correlation was observed between the number of nodes on the stem and the diameter of the grains. Regarding the variability of the determined characters, slightly higher values were found, mainly due to the existence of the dry bottom. And yet the Alvesta variety, with improved morphological characters proved to be a good adaptability to zonal cultivation conditions.

Influence of Super Absorbent Polymer and Humic Acid on Maize Yield and Nutrient Uptake on Rainfed Alfisols

The advances and development in agriculture depend not only on mechanization and new hybrid seeds but also on the improvement of soil physical and chemical properties which in turn help to increase crop productivity in dry land soils. A field study was carried out to study the influence of superabsorbent polymer at 2.5 and 4.5 kg ha-1and humic acid at 15 and 30 kg ha-1 alone and their combinations with recommended 100% fertilizers on yield, uptake, and photosynthetic pigments of maize grown on rainfed alfisols at Regional Agricultural Research Station, Palem, Telangana. The experiment was laid out in randomized block design with three replications, consisting of nine treatments. Further, results showed that conjoint application of [email protected] kg ha-1 + humic acid@30 kg ha-1 along with 100% RDF package significantly increased the pooled grain and stover yield (7136 and 8457 kg ha-1) of maize. Irrespective level of hydrogel and humic acid combinations with 100% RDF increased the macronutrient uptake by grain and stover, which further build up the total uptake. A similar pattern was also observed in corresponding to grain and stover yield.The chlorophyll “a”, “b” and total chlorophyll content (1.81, 1.69 1.54; 0.69, 0.62, 0.55 and 2.65, 2.46 and 2.24 mg g-1 in fresh plant weight at 30, 60 and 90 DAS) significantly influenced by application of [email protected] kg ha-1+ humic acid@30 kg ha-1. In conclusion, the present investigation indicates the positive interaction between humic acid and super absorbent polymer which improved nutrient uptake and maize yield.

SYMBIOTIC PROPERTIES OF BLACK MEDIC OF THE MIRA VARIETY

The first Russian variety of black medic of the Mira was created and included in the State Register of Breeding Achievements. The study of the symbiotic properties of this variety showed that the most promising strains for pre-sowing inoculation were LX1 and 412b, the first increased the collection of dry matter and seeds by 96 and 115%, and the second – by 81 and 73%, respectively. Both strains can be used when cultivating this variety for feed and seeds. Inoculation with the LX6 strain increased seed productivity by 84%. Strains LX2, LX5 and LX6 increase the adaptive ability of varieties of the Mira, shifting the metabolism of the alfalfa-rhizobial system towards increasing seed productivity. The proportion of seeds in plants inoculated with the above-mentioned strains increases to 24–31% of the total plant weight, and in other symbiotic systems this indicator was 19–22%. Strain LX1 was isolated from nodules of plants belonging to the same population on the basis of which the Mira variety was created and it is the most genetically complementary to this variety. The yield of black medic of the Mira variety without inoculation for two years of use was 3.3 t/ha of dry matter, 0.67 t/ha of seeds. Pre-sowing inoculation of seeds with the LX1 strain increased the yield of this variety to 6.48 t/ha (+96%) of dry matter and seeds – to 1.44 t/ha (+115%). In the vegetation experiment, inoculation with the rhizobium strain LX1 significantly increased the height of plants by 4.2 cm, the leaf area by 0.69 cm2 (+49%), productivity by 176%. The total nitrogen content in the aboveground part of the plants reached 3.63%, the roots 3.31%, the control indicators were 3.02 and 2.77%, respectively.

Identification and Management of Phytophthora Aerial Blight Caused by Phytophthora nicotianae on Catharanthus roseus

Phytophthora nicotianae is the most common pathogen in nurseries and gardens, infecting both woody and herbaceous ornamental plants. Phytophthora aerial blight symptoms such dull water-soaked lesions on shoot tips and leaf petioles, girdling on the main stem, necrosis, and wilting of annual vinca were observed in a commercial greenhouse in Warren Co., Tennessee, USA in May 2016. The objective of this study was to identify the causal agent of Phytophthora aerial blight and develop a fungicide management recommendation for ornamental producers. Attempts to isolate the pathogen from symptomatic leaf tissue were conducted and excised leaf pieces were embedded in the V8 agar medium. Morphological characterization, polymerase chain reaction (PCR), sequencing, and pathogenicity test of the isolate FBG2016_444 were conducted to confirm the pathogen identification. The sequence identity was 100% identical to Phytophthora nicotianae, and a combined phylogenetic tree (internal transcribed spacer [ITS]), the large subunit [LSU] of rDNA, and ras-related protein gene [Ypt1]) grouped isolate FBG2016_444 within the clade of P. nicotianae. In the pathogenecity study, all inoculated annual vinca plant showed the Phytophthora aerial blight symptoms and P. nicotianae was re-isolated whereas non-inoculated annual vinca plant remained symptomless. These findings confirmed P. nicotianae as the causal agent of Phytophthora aerial blight of annual vinca. In addition, two rates (0.078 and 0.156 mL·L-1) and three application intervals (7, 14 and 21 days before inoculation [DBI]) of oxathiapiprolin (Segovis®) were evaluated for their ability to reduce the Phytophthora aerial blight severity on annual vinca plants. The control groups were positive (non-treated inoculated) and negative (non-treated non-inoculated) plants. Both rates and application timings of oxathiapiprolin significantly reduced Phytophthora aerial blight severity and disease progress (area under disease progress curve [AUDPC]) on annual vinca plants compared to the positive control. However, 0.078 and 0.156 mL·L-1 of oxathiapiprolin applied at 7 or 14 DBI were the most effective treatments in reducing the disease severity and AUDPC on annual vinca plants. The plant growth parameters such as increase in height and width, total plant weight, and root weight were not influenced by the application of oxathiapiprolin. The finding reported in this study will help ornamental growers with better management of Phytophthora aerial blight of annual vinca.

Gypsum Supplies Calcium to Ultisol Soil and Its Effect on Pineapple Growth, Yield and Fruit Quality in Lower Single Bed under Climate Change Issue

A lower bed single row for pineapple cultivation could protect pineapple from soil erosion in rainy season and during drought, however, disease problem could arise due to water logging. Two experiments using a lower bed single row was done to understand the ability of gypsum providing soil calcium (Ca) available to pineapple plant, resistance to heart rot disease, and give better effect on crop growth and fruit quality of the pineapple in Ultisol soil. In the first trial, four level dosis of gypsum (0, 1.0, 1.5, 2.0 Mg ha-1) and dolomite 2 Mg ha-1 were applied by spreading and incorporated into the soil which have saturated with inoculums of Phytophthora nicotianae. In the second trial, gypsum treatments (0, 1.0, 1.5, 2.0, 2.5 Mg ha-1) were applied in the row between the single row beds as a basic fertilizer. The result showed that P. nicotianae attacked the pineapple plants in all treatments at 6 weeks after planting (WAP), and at 10 WAP, the mortality of dolomite treatment reached 63.8%, significantly different than that for gypsum treatments (3.3-14.3%). In the second experiment, gypsum increased plant weight significantly at 3 until 9 months after planting especially when it was applied 1.5-2.5 Mg ha-1. Fruit texture, total soluble solid (TSS), titratable acidity (TA) were not significant different among the treatment but all meet the standards for grades of canned pineapple. Result showed that soil applied gypsum before planting provides soil calcium and met the plant Ca requirement during a period of early and fast growth step and safe for heart rot disease.

Humic acids impact on heavy metal phytoextraction in Tagetes patula L.

In a model study, we analysed the impact of humic acids (HAs, 500 ppm) on the accumulation of heavy metals (HMs; Pb, Cu, Ni and Zn) in roots and aboveground organs of the annual ornamental plant Tagetes patula (Scarlet variety) cultivated on the Terra Vita nutritive substrate used in modern cultural landscape mulching to imitate the upper fertile root layer (UR-RAT) of contaminated urban soils in megacities. Plants were grown in full photoculture (at no sunlight) in pots placed in a closed grow box, maintaining internal microclimate. A modern HLG Quantum Board QB288 V2 Rspec LED panel enabled with vegetation modes was used as light source. HMs were introduced in substrate as aqueous saline. Each setting was quadruplicated. Total experiment duration was 30 days. Tagetes patula was proved a particularly effective phytoextractor of Zn applied at 40 mg/kg (p<0.01). At the same time, the plants showed high tolerance to toxic growth inhibition (in biometry of the above- and underground organ length and mass) and preserved external aesthetics. Metals were further graded by phytomass accumulation as follows: Cu>Pb>Ni. Ni at 30 mg/kg had a major impact on plant weight and length, which sets off the variety as a sensitive phytoexcluder for this metal. HAs increased aboveground mass in all settings and decreased the HM mass impact. A sharp root mass reduction was observed in combinations of the two factors (HMs+HAs), which was clearly reflected in impaired root mass in zinc settings. A single metal addition reduced the mass by average 12.0 % (p<0.01) vs. control (no HAs or HMs), whilst a combined HAs+HMs administration induced its 65.0 % reduction (p<0.01). The results obtained indicate a good root buffering capacity for HM translocation from the rhizosphere to aboveground parts. In general, HA usage is promising for creating the green space and phytoremediation of urban HM-contaminated soils.

Evaluation of Chemical Seed Treatment to Reduce Injury Caused by Preemergent Herbicides on Direct-seeded Turnips and Collard Greens

Poor competitive ability and limited herbicide options make weed management of Brassica crops difficult. Growers often adopt the use of transplants, which is less efficient in terms of time, material, and labor when compared with direct seeding, resulting in higher prices per unit. Seed treatment with protective compounds could decrease crop injury from preemergent (PRE) herbicides making it profitable to direct-seed Brassica plants for production. Research was conducted to evaluate the ability of three candidate safeners [24-epibrassinolide, melatonin, and ascorbic acid (AsA)] to reduce injury caused by four herbicides (S-metolachlor, pyroxasulfone, halosulfuron, and mesotrione) applied PRE on the collard green cultivar Top Bunch and turnip cultivar Purple Top White Globe. Two independent greenhouse trials were conducted at the Clemson University Coastal Research and Education Center in Charleston, SC. Visual injury of the treated plants was evaluated weekly and dry mass was collected 21 days after treatment. Seed treatment did not reduce injury efficiently caused by pyroxasulfone, halosulfuron, and mesotrione; all doses were lethal for both crops. However, collard seeds treated using melatonin and AsA had 66% and 54% less injury caused by S-metolachlor at 514 g⋅ha–1 a.i., respectively. On turnips, melatonin was the only treatment that reduced the S-metolachlor damage on seedlings, with 43% less injury than untreated seedlings. Plant injury and plant weight correlated significantly for both Brassica crops. The reduction in injury caused by S-metolachlor when seeds were treated with melatonin and AsA validated those compounds’ protective ability. Seed treatment with melatonin could be combined with PRE applications of S-metolachlor to overcome the low weed competitive ability of these species early in the season.

Allelopathic potential of siam weed (Chromolaena odorata L.) extract for enhancing soybean productivity

Many tropical invasive species have strong allelopathic effects. Chromolaena odorata is reported to have the best potential to act as bioherbicide against several weeds on crops. This research was conducted in May-September 2019 to evaluate the efficacy of C. odorata extract on soybean plant. This study employed Randomized Block Design (RBD) Factorial with two factors. The first factor was 4 different doses of C. odorata extracts: 0, 8, 16 and 24 tons ha−1. The second factor was 3 different times of application: 0, 7 and 14 days after planting. Variables observed were number of pods per plant, seed weight per plant, weight of 100 seeds and dry seed yield. The findings indicated that the extract did have effect on seed weight per plant, weight of 100 seeds and yield of dry seeds. The application of extract 16-24 tons ha−1 has improved the seed weight per plant, weight of 100 seeds, and yield of dry seeds. There was no interaction between siam weed extract and time of application in all variables observed.