Seed genetic purity testing of F1 hybrid cabbage (Brassica oleracea var. capitata) with molecular marker analysis

2007 ◽  
Vol 35 (2) ◽  
pp. 477-486 ◽  
Author(s):  
G. Liu ◽  
L. Liu ◽  
Y. Gong ◽  
Y. Wang ◽  
F. Yu ◽  
...  
2008 ◽  
pp. 231-238 ◽  
Author(s):  
L. Liu ◽  
Y. Wang ◽  
Y. Gong ◽  
X. Zhai ◽  
F. Yu ◽  
...  

2020 ◽  
Vol 48 (3) ◽  
pp. 345-353
Author(s):  
J.Y. Chen ◽  
Q.M. Chen ◽  
Z.G. Liu ◽  
C.L. Wang ◽  
L.L. Ma ◽  
...  

To ensure that farmers can access high-quality seeds, it is essential to find a simple, rapid and accurate method to assess seed purity. In recent years, heterosis in chieh-qua has been widely applied to production. Using the whole genome sequence of chieh-qua, we designed simple sequence repeat (SSR) primers specific for chieh-qua. The parental lines of nine hybrids were screened using 200 SSR primers, seven of which exhibited polymorphisms. The bands were clear, stable and reproducible. We found dominant and co-dominant bands between the parents of the nine hybrids. The seven pairs of SSR primers were successfully used to assess genetic purity of the nine chieh-qua hybrids. The SSR molecular marker purity assessment results were consistent with the results obtained from a field grow-out test (GOT). However, the use of SSR markers provided a more accurate, reliable and faster method for seed purity testing than the GOT. We propose using SSR molecular marker technology to assess the genetic purity of chieh-qua hybrid seeds. With this method, the seed quality can be determined faster, which may help to accelerate the chieh-qua breeding process.


2013 ◽  
Vol 155 ◽  
pp. 92-96 ◽  
Author(s):  
Shan Ye ◽  
Yan Wang ◽  
Danqiong Huang ◽  
Jianbin Li ◽  
Yiqin Gong ◽  
...  

2014 ◽  
Vol 13 (40) ◽  
pp. 3999-4007 ◽  
Author(s):  
D. N. Njoku, ◽  
V. E Gracen, ◽  
S. K. Offei, ◽  
I. K. Asante, ◽  
E. Y. Danquah, ◽  
...  

2011 ◽  
Vol 34 (1) ◽  
pp. 88-102 ◽  
Author(s):  
Juliana Morini Küpper Cardoso Perseguini ◽  
Alisson Fernando Chioratto ◽  
Maria Imaculada Zucchi ◽  
Carlos Augusto Colombo ◽  
Sérgio Augusto Moraes Carbonell ◽  
...  

2019 ◽  
Vol 13 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Kai-Dong Xie ◽  
Qiang-Ming Xia ◽  
Jun Peng ◽  
Xiao-Meng Wu ◽  
Zong-Zhou Xie ◽  
...  

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