Cyproterone acetate (CA) may induce the synthesis of androgen receptors (AR) in rat ventral prostate independently of receptor translocation to the nucleus

1984 ◽  
Vol 104 (4_Supplb) ◽  
pp. S94-S95 ◽  
Author(s):  
H. MOELLER ◽  
B. GOECKE
Keyword(s):  
Cyproterone Acetate ◽  
Androgen Receptors ◽  
Ventral Prostate ◽  
Rat Ventral Prostate

Expression of androgen receptors and prostatic steroid-binding protein during development of the rat ventral prostate

1988 ◽  
Vol 117 (3) ◽  
pp. 361-NP ◽  
Author(s):  
Y. L. Zhang ◽  
Z. X. Zhou ◽  
Y. D. Zhang ◽  
M. G. Parker
Keyword(s):  
Gene Expression ◽  
Androgen Receptors ◽  
Binding Protein ◽  
Transient Increase ◽  
Neonatal Rat ◽  
Ventral Prostate ◽  
Rat Ventral Prostate ◽  
Old Rats ◽  
Steroid Binding ◽  
Steroid Binding Protein

ABSTRACT Prostatic steroid-binding protein (PSBP) mRNAs transcribed from the three genes C1, C2 and C3 were quantitated in neonatal rat ventral prostate by Northern blot analysis. Transcription was initiated at day 14 for C1 and C2 and day 10 for C3, and reached mature levels by day 21 for C1 and C2 and day 28 for C3. The changes of both cytoplasmic and nuclear prostatic androgen receptors in 10- to 150-day-old rats were investigated by radioligand assay and showed a fivefold transient increase between days 10 and 28. Thus there was a good correlation between the onset of PSBP gene expression and the transient increase in androgen receptors; increases in receptor concentration may be a prerequisite for changes in gene expression. J. Endocr. (1988) 117, 361–366

Molecular cloning of human and rat complementary DNA encoding androgen receptors

Science ◽  
1988 ◽  
Vol 240 (4850) ◽  
pp. 324-326 ◽  
Author(s):  
CS Chang ◽  
J Kokontis ◽  
ST Liao
Keyword(s):  
Androgen Receptors ◽  
Steroid Receptors ◽  
Ventral Prostate ◽  
Cdna Libraries ◽  
Human Testis ◽  
Dna Binding Domain ◽  
Dna Encoding ◽  
High Affinity ◽  
Autoimmune Antibodies ◽  
Rat Ventral Prostate

Complementary DNAs (cDNAs) encoding androgen receptors were obtained from human testis and rat ventral prostate cDNA libraries. The amino acid sequence deduced from the nucleotide sequences of the cDNAs indicated the presence of a cysteine-rich DNA-binding domain that is highly conserved in all steroid receptors. The human cDNA was transcribed and the RNA product was translated in cell-free systems to yield a 76-kilodalton protein. The protein was immunoprecipitable by human autoimmune antibodies to the androgen receptor. The protein bound androgens specifically and with high affinity.

EFFECT OF PROLACTIN, GROWTH HORMONE AND INSULIN ON THE UPTAKE AND BINDING OF DIHYDROTESTOSTERONE TO THE CULTURED RAT VENTRAL PROSTATE

1976 ◽  
Vol 81 (4) ◽  
pp. 854-864 ◽  
Author(s):  
Risto Johansson
Keyword(s):  
Growth Hormone ◽  
Cyproterone Acetate ◽  
Specific Binding ◽  
Ventral Prostate ◽  
Free Form ◽  
Nuclear Fraction ◽  
Total Uptake ◽  
Rat Ventral Prostate ◽  
Cell Components ◽  
Specific Uptake

ABSTRACT The effects of prolactin, growth hormone and insulin on the total uptake and specific binding of tritiated dihydrotestosterone in the cultured rat ventral prostate were examined. In similar conditions prolactin and insulin act synergistically with testosterone on the macromolecule synthesis of the prostate, but have no effect on the conversion of testosterone to dihydrotestosterone. The total uptake of tritiated dihydrotestosterone to the tissues was slightly, but not statistically significantly, increased by prolactin, insulin and growth hormone. The majority of the radioactive dihydrotestosterone in the tissue was in free form or very loosely bound. None of these three hormones altered the binding of tritiated dihydrotestosterone to the cytoplasmic receptors. Non-radioactive dihydrotestosterone, cyproterone and cyproterone acetate in 1000 foid excess strongly decreased the binding of tritiated dihydrotestosterone to the cytoplasmic reseptors and to the nuclei. That part of the binding, which was inhibited by the hormones was considered to represent the specific binding to the receptors. Insulin stimulated both the specific and the unspecific uptake of dihydrotestosterone to the nuclei. Prolactin only stimulated the specific uptake to the nuclei while growth hormone had no effect. Autoradiography of the nuclear fraction indicated a firm binding of tritiated dihydrotestosterone to the nuclei. The radioactivity of the other contaminating cell components in this fraction appeared to be negligible.

Androgen receptors in rat ventral prostate microsomes

1974 ◽  
Vol 373 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Paul Robel ◽  
Jean-Paul Blondeau ◽  
Etienne-Emile Baulieu
Keyword(s):  
Androgen Receptors ◽  
Ventral Prostate ◽  
Rat Ventral Prostate

Phosphorylation status of nuclear and cytosolic androgen receptors in the rat ventral prostate

The Prostate ◽  
1989 ◽  
Vol 14 (2) ◽  
pp. 91-101 ◽  
Author(s):  
E. J. Golsteyn ◽  
J. S. Graham ◽  
H. J. Goren ◽  
Y. A. Lefebvre
Keyword(s):  
Androgen Receptors ◽  
Ventral Prostate ◽  
Rat Ventral Prostate

scholarly journals Testosterone action in the rat ventral prostate. The effects of diethylstilboestrol and cyproterone acetate on the metabolism of [3H]testosterone and the retention of labelled metabolites by rat ventral prostate in vivo and in vitro

1971 ◽  
Vol 125 (1) ◽  
pp. 81-91 ◽  
Author(s):  
J. E. Belham ◽  
G. E. Neal
Keyword(s):  
Cyproterone Acetate ◽  
Prostatic Carcinoma ◽  
Prostate Gland ◽  
Target Organ ◽  
Ventral Prostate ◽  
Nuclear Retention ◽  
Rat Ventral Prostate ◽  
Androgenic Effect

Recent reports have indicated that the prior metabolism of testosterone by the secondary sexual tissues may be necessary for its androgenic effect. The effects of two anti-androgens, diethylstilboestrol and cyproterone acetate (17α-acetoxy-6-chloro-1,2α-methylenepregna-4,6-diene-3,20-dione) used in the chemotherapy of human prostatic carcinoma, have been examined on both the metabolism of testosterone and the retention of its metabolites by the rat ventral prostate gland. Cyproterone acetate was found to inhibit the retention of labelled metabolites of [3H]-testosterone by prostatic nuclei, both in vivo and in vitro. This inhibition appeared to be competitive. In contrast with its effect on nuclear retention of metabolites of testosterone, cyproterone acetate had no significant effect on the metabolism of [3H]testosterone by rat ventral prostate tissue. Diethylstilboestrol similarly had little effect on the metabolism of [3H]testosterone by prostatic tissue, although it did appear partially to inhibit its initial metabolism in all the incubation systems used. Diethylstilboestrol inhibited the nuclear retention of dihydrotestosterone when both [3H]testosterone and diethylstilboestrol were injected intraperitoneally in vivo, but had no effect on dihydrotestosterone retention when both testosterone and diethylstilboestrol were supplied directly to the prostate either in vivo or in vitro. It was concluded that if diethylstilboestrol has an anti-androgenic effect at the level of the target organ as distinct from its effect on androgen production by the testes, then it is probably due to a mechanism differing from that of cyproterone acetate.

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