scholarly journals Analysis of the MYD88 L265P mutation in IgM monoclonal gammopathy by semi-nested polymerase chain reaction-based restriction fragment length polymorphism method

2021 ◽  
Vol 33 (2) ◽  
pp. 47-54
Author(s):  
Shun Fujiwara ◽  
Yuta Baba ◽  
Yohei Sasaki ◽  
Shotaro Shimada ◽  
So Murai ◽  
...  
2021 ◽  
Vol 15 (10) ◽  
pp. 1539-1546
Author(s):  
Samira Metwally Mohammad ◽  
Magda Saad Ali ◽  
Sara Ahmed Abdel-Rahman ◽  
Raghda Abdelrahman Moustafa ◽  
Mohamed Hassan Sarhan

Introduction: The protozoan parasite Cryptosporidium is one of the principal reasons for childhood diarrhea around the world. This work aimed to differentiate Cryptosporidium species among children suffering from diarrhea in Sharkyia Governorate, Egypt. Methodology: A total of 97 fecal specimens were taken from children suffering from diarrhea, attending Pediatric Clinics of Zagazig University and Al-Ahrar Hospitals. Full history was taken. Stool samples were examined microscopically using modified Ziehl–Neelsen stain for detection of Cryptosporidium oocysts. To identify Cryptosporidium genotypes, positive samples were then subjected to nested Polymerase chain reaction-restriction fragment length polymorphism targeting Cryptosporidium oocyst wall protein gene. Results: The overall detection rate was 27.8% (27/97) using modified Ziehl–Neelsen stain staining method. Using nested polymerase chain reaction, the gene was amplified in 85.2% (23/27). Restriction fragment length polymorphism analysis revealed that 65.2% (15/23) were Cryptosporidium hominis, 30.4% (7/23) were Cryptosporidium parvum, and one sample was not typed (4.4%). The significant risk factors associated with Cryptosporidium infection in children were animal contact and residence in rural areas. Conclusions: Cryptosporidium is a common enteric parasite affecting children in Sharkyia Governorate, Egypt, with the predominance of C. hominis genotype in children.


2001 ◽  
Vol 47 (10) ◽  
pp. 903-907
Author(s):  
Richard Parkes ◽  
Teresa Lo ◽  
Quantine Wong ◽  
Judith L Isaac-Renton ◽  
Sean K Byrne

A nested polymerase chain reaction – restriction fragment length polymorphism (PCR–RFLP) method, the PATH antigen detection method, and light microscopy were compared for their capacity to detect and identify Plasmodium species. One hundred and thirty-six blood specimens obtained from patients suspected of having malaria were examined by each of the three methods. Forty-four specimens were positive for malaria using microscopy as the "gold standard". The sensitivity for nested PCR was 100%, and the specificity was 98%. For the detection of Plasmodium falciparum, the antigen detection method had a sensitivity of 100% and a specificity of 97%. Species identification obtained using PCR–RFLP was identical or superior to light microscopy in 42 cases (96%). Although the nested PCR–RFLP method was more sensitive and specific, the rapid turnaround time and high sensitivity of the antigen detection method makes it a useful adjunct to standard microscopy.Key words: malaria, PCR–RFLP, antigen detection.


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