Determination of the rdw Rat Genotype by Polymerase Chain Reaction with Allele-Specific Primer (PCR-ASP)

2004 ◽  
Vol 53 (2) ◽  
pp. 155-157
Author(s):  
Tamotsu MASAKI ◽  
Ken TAKAMATSU ◽  
Motoaki UMEZU
Keyword(s):  
Polymerase Chain Reaction ◽  
Specific Primer ◽  
Chain Reaction ◽  
Allele Specific ◽  
Polymerase Chain

Rapid determination of platelet alloantigen genotypes by polymerase chain reaction using allele-specific primers

Transfusion ◽  
1994 ◽  
Vol 34 (11) ◽  
pp. 955-960 ◽  
Author(s):  
B Skogen ◽  
DB Bellissimo ◽  
MJ Hessner ◽  
S Santoso ◽  
RH Aster ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapid Determination ◽  
Chain Reaction ◽  
Specific Primers ◽  
Allele Specific ◽  
Polymerase Chain

Determination of carrier status in Duchenne and Becker muscular dystrophies by quantitative polymerase chain reaction and allele-specific oligonucleotides

1990 ◽  
Vol 36 (12) ◽  
pp. 2113-2117 ◽  
Author(s):  
T W Prior ◽  
A C Papp ◽  
P J Snyder ◽  
W E Highsmith ◽  
K J Friedman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Muscular Dystrophy ◽  
Gene Dosage ◽  
Quantitative Polymerase Chain Reaction ◽  
Becker Muscular Dystrophy ◽  
Carrier Status ◽  
Chain Reaction ◽  
Allele Specific ◽  
Polymerase Chain

Abstract Detection of carriers of Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), in the deletion cases, involves calculating gene dosage from Southern blots. We show that the analysis of dosage can also be made from the polymerase chain reaction (PCR) with use of allele-specific oligonucleotides (ASOs). The deletion-prone exons are amplified, transferred to a membrane, and hybridized with ASOs complementary to the exons; the autoradiographic bands are then quantified with a densitometer. After determining the quantitative conditions of the amplification reaction, we were able to identify deletions in a DMD/BMD carrier female. The determination of carrier status via PCR removes several of the technical limitations of Southern analysis and is also cost- and labor-effective.

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