Data processing algorithms for the in silico SARS-CoV-2 epitope prediction and vaccine development
Based on literature analysis and own bioinformatics and virology research experience, authors propose multistep data processing algorithms, designed for the objectives of assisting the SARS-CoV-2 epitope vaccine production. Epitope vaccines are expected to provoke a weaker but safer response of the vaccinated person. Methodologies of reverse bioengineering, vaccinology and synthetic peptide manufacturing have a promising future to combat COVID-19 brutal disease. The significant mutational variability and evolution of the SARS-CoV-2, which is more typical for natural animal-borne viruses, are the hurdle for the effective and robust vaccine application and therefore require multidisciplinary research and prevention measures on the international level of cooperation. However, we can expect that other viruses with different nature and content may be labelled as SARS-CoV-2. In this case metagenomics is an important discipline for COVID-19 discovery. High quality reliable virus detection is still an unresolved question for improvement and optimization. It is of upmost importance to develop the in silico and in vitro methods for the vaccine recipient reaction prediction and monitoring as techniques of the so-called modern personalized medicine. Many questions can`t be solved applying exclusively in silico techniques and only can be discovered in vitro and in vivo, demanding significant time and money investments. Future experiments also should be directed at the discovery of optimal vaccine adjuvants, vectors and epitope ensembles, as well as the personal characteristics of citizens of a certain region. This research would require several more years of meticulous large-scale laboratory and clinical work in various centers of biomedical institutions worldwide