scholarly journals Szabadgyök-felszabadulás vizsgálata femtoszekundum lézerrel asszisztált capsulotomiát követően

2016 ◽  
Vol 157 (47) ◽  
pp. 1880-1883 ◽  
Author(s):  
Gábor Tóth ◽  
Gábor László Sándor ◽  
Dénes Kleiner ◽  
Nóra Szentmáry ◽  
Huba J. Kiss ◽  
...  
Keyword(s):  
Free Radical ◽  
Femtosecond Laser ◽  
Anterior Chamber ◽  
Treatment Method ◽  
Interquartile Range ◽  
Control Group ◽  
Relative Light Unit ◽  
Free Radical Production ◽  
Radical Production ◽  
Group A

Introduction: Femtosecond laser is a revolutionary, innovative treatment method used in cataract surgery. Aim: To evaluate free radical quantity in the anterior chamber of the eye, during femtosecond laser assisted capsulotomy, in a porcine eye model. Method: Seventy fresh porcine eyes were collected within 2 hours post mortem, were transported at 4 ºC and treated within 7 hours. Thirty-five eyes were used as control and 35 as femtosecond laser assisted capsulotomy group. A simple luminol-dependent chemiluminescence method was used to measure the total scavenger capacity in the aqueous humour, as an indicator of free radical production. The emitted photons were expressed in relative light unit %. Results: The relative light unit % was lower in the control group (median 1%, interquartile range [0.4–3%]) than in the femtosecond laser assisted capsulotomy group (median 4.4%, interquartile range [1.5%–21%]) (p = 0.01). Conclusions: Femtosecond laser assisted capsulotomy decreases the antioxidant defense of the anterior chamber, which refers to a significant free radical production during femtosecond laser assisted capsulotomy. Orv. Hetil., 2016, 157(47), 1880–1883.

Free radical production by femtosecond laser lens irradiation in porcine eyes

2019 ◽  
Vol 45 (8) ◽  
pp. 1168-1171 ◽  
Author(s):  
Yoichiro Masuda ◽  
Tsutomu Igarashi ◽  
Kotaro Oki ◽  
Maika Kobayashi ◽  
Hiroshi Takahashi ◽  
...  
Keyword(s):  
Free Radical ◽  
Femtosecond Laser ◽  
Free Radical Production ◽  
Radical Production ◽  
Porcine Eyes

Chemiluminescence as an index of drug-induced free radical production in pancreatic islets

Diabetes ◽  
1984 ◽  
Vol 33 (2) ◽  
pp. 160-163 ◽  
Author(s):  
K. Asayama ◽  
D. English ◽  
A. E. Slonim ◽  
I. M. Burr
Keyword(s):  
Free Radical ◽  
Pancreatic Islets ◽  
Drug Induced ◽  
Free Radical Production ◽  
Radical Production

Features of the oxidative status in patients with lupus nephritis

2020 ◽  
Vol 24 (1) ◽  
pp. 39-44
Author(s):  
E. V. Smirnova ◽  
E. V. Proskurnina ◽  
T. N. Krasnova
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Lupus Nephritis ◽  
Blood Plasma ◽  
Respiratory Burst ◽  
Oxidative Status ◽  
Free Radical Production ◽  
Radical Production ◽  
Neutrophil Activity ◽  
Kidney Involvement

BACKGROUND. Oxidative status impairment plays a significant role in the pathogenesis of SLE and lupus nephritis (LN). The data about oxidative status in this disease are incomplete, that’s why it’s necessary to use a new approach to study it. THE AIM: To study oxidative status in SLE patients with kidney involvement. PATIENTS AND METHODS:53 patients with SLE were included in this prospective study, among them 40 patients with different severity of kidney involvement, control group were 87 healthy donors. Oxidative stress parameters were measured: antioxidant activity (AOA) of blood plasma and parameters, characterizing the state of the main source of reactive oxygen species (ROS) – neutrophils, more specifically: specific spontaneous neutrophil activity, specific stimulated activity (peak and integral), coefficient of respiratory burst attenuation, representing the rate of free radical production decrease after stimulation, the higher the value of this parameter, the slower is free radical production decrease. RESULTS. It was shown elevation of neutrophil free radical-producing activity parameters and elevation of blood plasma AOA in patients with LN, comparing to healthy controls. Immunosuppressive therapy with glucocorticosteroids (GCS) and cytostatics (CS) increased blood plasma AOA comparing to monotherapy with GCS. A correlation between oxidative status impairment and intensity of inflammatory reactions was found: correlation of respiratory burst attenuation coefficient with blood sedimentation rate was shown. Reduction of spontaneous free radical-producing neutrophil activity was found in LN patients with NS, which might be the result of neutrophil functional activity attenuation in high disease activity. CONCLUSION. The increased free radical-producing neutrophil activity was shown, which might be the cause of oxidative stress in SLE with LN. It seems warranted investigation of these parameters in samples of larger volume to search targets aimed at neutrophils. The necessity of antioxidant therapy in patients with SLE seems doubtful, as they show significant increase of blood plasma AOA, which might result from compensatory reaction of human organism to oxidative stress and therapy with GCS and CS.

Free radical production in the rat retina

1992 ◽  
Vol 55 ◽  
pp. 248
Author(s):  
H. Zhang ◽  
E. Agardh ◽  
C-D. Agardh
Keyword(s):  
Free Radical ◽  
Rat Retina ◽  
Free Radical Production ◽  
Radical Production

Glutamine-induced free radical production in cultured astrocytes

Glia ◽  
2004 ◽  
Vol 46 (3) ◽  
pp. 296-301 ◽  
Author(s):  
Arumugam R. Jayakumar ◽  
K.V. Rama Rao ◽  
Arne Schousboe ◽  
Michael D. Norenberg
Keyword(s):  
Free Radical ◽  
Free Radical Production ◽  
Radical Production ◽  
Cultured Astrocytes

scholarly journals Direct evidence of iNOS-mediated in vivo free radical production and protein oxidation in acetone-induced ketosis

2008 ◽  
Vol 295 (2) ◽  
pp. E456-E462 ◽  
Author(s):  
Krisztian Stadler ◽  
Marcelo G. Bonini ◽  
Shannon Dallas ◽  
Danielle Duma ◽  
Ronald P. Mason ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Nadph Oxidase ◽  
Knockout Mice ◽  
Protein Oxidation ◽  
Ketone Bodies ◽  
Free Radical Production ◽  
Radical Production ◽  
Inos Knockout Mice

Diabetic patients frequently encounter ketosis that is characterized by the breakdown of lipids with the consequent accumulation of ketone bodies. Several studies have demonstrated that reactive species are likely to induce tissue damage in diabetes, but the role of the ketone bodies in the process has not been fully investigated. In this study, electron paramagnetic resonance (EPR) spectroscopy combined with novel spin-trapping and immunological techniques has been used to investigate in vivo free radical formation in a murine model of acetone-induced ketosis. A six-line EPR spectrum consistent with the α-(4-pyridyl-1-oxide)- N-t-butylnitrone radical adduct of a carbon-centered lipid-derived radical was detected in the liver extracts. To investigate the possible enzymatic source of these radicals, inducible nitric oxide synthase (iNOS) and NADPH oxidase knockout mice were used. Free radical production was unchanged in the NADPH oxidase knockout but much decreased in the iNOS knockout mice, suggesting a role for iNOS in free radical production. Longer-term exposure to acetone revealed iNOS overexpression in the liver together with protein radical formation, which was detected by confocal microscopy and a novel immunospin-trapping method. Immunohistochemical analysis revealed enhanced lipid peroxidation and protein oxidation as a consequence of persistent free radical generation after 21 days of acetone treatment in control and NADPH oxidase knockout but not in iNOS knockout mice. Taken together, our data demonstrate that acetone administration, a model of ketosis, can lead to protein oxidation and lipid peroxidation through a free radical-dependent mechanism driven mainly by iNOS overexpression.

scholarly journals Intraischaemic hypothermia reduces free radical production and protects against ischaemic insults in cultured hippocampal slices

2004 ◽  
Vol 91 (2) ◽  
pp. 327-336 ◽  
Author(s):  
Terence McManus ◽  
Matthew Sadgrove ◽  
Ashley K. Pringle ◽  
John E. Chad ◽  
Lars E. Sundstrom
Keyword(s):  
Free Radical ◽  
Hippocampal Slices ◽  
Free Radical Production ◽  
Radical Production
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