Rapid detection ofFusarium graminearumcomplex in wheat seeds using species-specific PCR primer designed based on a microsatellite region

2012 ◽  
Vol 40 (1) ◽  
pp. 85-94 ◽  
Author(s):  
M. Hamada ◽  
Y. Yin ◽  
Z. Ma
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Specific Pcr Primer ◽  
Species Specific ◽  
Wheat Seeds ◽  
Pcr Primer

A simple method for discriminating Bursaphelenchus xylophilus and B. mucronatus by species-specific polymerase chain reaction primer pairs

Nematology ◽  
2004 ◽  
Vol 6 (2) ◽  
pp. 273-277 ◽  
Author(s):  
Koji Matsunaga ◽  
Katsumi Togashi
Keyword(s):  
Pcr Amplification ◽  
Nematode Species ◽  
Bursaphelenchus Xylophilus ◽  
Polymerase Chain Reaction Primer ◽  
Dna Fragments ◽  
Simple Method ◽  
Specific Pcr ◽  
Pcr Products ◽  
Specific Pcr Primer ◽  
Species Specific

Abstract Two species-specific PCR primer pairs were developed for identifying the two nematode species, Bursaphelenchus xylophilus and B. mucronatus. The primer pairs were developed from the sequence of ribosomal DNA (rDNA) repeats to produce DNA fragments of different lengths by PCR amplification. The DNA fragments for B. mucronatus and B. xylophilus were 210 bp and 557 bp, respectively. When mixed, neither primer pair inhibited the PCR amplification of the other. Five isolates of B. xylophilus and four isolates of B. mucronatus showed different band profiles of PCR products between the two species, but identical profiles among isolates of the same species.

scholarly journals An improved allele-specific PCR primer design method for SNP marker analysis and its application

Plant Methods ◽  
2012 ◽  
Vol 8 (1) ◽  
pp. 34 ◽  
Author(s):  
Jing Liu ◽  
Shunmou Huang ◽  
Meiyu Sun ◽  
Shengyi Liu ◽  
Yumei Liu ◽  
...  
Keyword(s):  
Design Method ◽  
Primer Design ◽  
Snp Marker ◽  
Specific Pcr ◽  
Marker Analysis ◽  
Allele Specific ◽  
Specific Pcr Primer ◽  
Allele Specific Pcr ◽  
Pcr Primer

Rapid detection of fraudulence in seven commercial shrimp products by species-specific PCR assays

Food Control ◽  
2021 ◽  
Vol 124 ◽  
pp. 107871
Author(s):  
Lidiya Wilwet ◽  
Robinson Jeya Shakila ◽  
Balasubramanian Sivaraman ◽  
Binaya Bhusan Nayak ◽  
H. Sanath Kumar ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Pcr Assays ◽  
Species Specific

Rapid detection and identification of Streptococcus ratti by a species-specific PCR method

Anaerobe ◽  
2012 ◽  
Vol 18 (1) ◽  
pp. 44-47
Author(s):  
Jumpei Nishio ◽  
Makoto Taniguchi ◽  
Juichiro Higashi ◽  
Masakazu Takahashi ◽  
Takuma Ando ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Detection And Identification ◽  
Pcr Method ◽  
Species Specific ◽  
Streptococcus Ratti

Species-specific PCR-based marker for rapid detection of Aspidiotus rigidus Reyne (Hemiptera: Diaspididae)

2022 ◽  
Vol 25 (1) ◽  
pp. 101848
Author(s):  
Romnick A. Latina ◽  
Darlon V. Lantican ◽  
Michelle S. Guerrero ◽  
Edsel C. Rubico ◽  
Janice F. Laquinta ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Species Specific

Updating specific PCR primer for detection of Cryptocaryon irritans from reared Larimichthys polyactis

2021 ◽  
Vol 223 ◽  
pp. 108081
Author(s):  
Xiao Xie ◽  
Chao Zheng ◽  
Aysha Zahid ◽  
Jindong Kong ◽  
Bushra ◽  
...  
Keyword(s):  
Larimichthys Polyactis ◽  
Cryptocaryon Irritans ◽  
Specific Pcr ◽  
Specific Pcr Primer ◽  
Pcr Primer

Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridisation

2003 ◽  
Vol 81 (3) ◽  
pp. 231-239 ◽  
Author(s):  
Marina Papadelli ◽  
Eugenia Manolopoulou ◽  
George Kalantzopoulos ◽  
Effie Tsakalidou
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Detection And Identification ◽  
Dna Hybridisation ◽  
Species Specific
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