Rapid detection of fraudulence in seven commercial shrimp products by species-specific PCR assays

Heterodera avenae and H. filipjevi are economically important cyst nematodes that restrict production of cereal crops in the Pacific Northwest United States and elsewhere in the world. Identification of these two species is critical for recommending and implementing effective management practices. Primers were designed from the internal transcribed spacer (ITS) regions of H. avenae and H. filipjevi ribosomal DNA. The primers were highly specific when examined on target isolates but did not amplify DNA from nontarget Heterodera, Globodera, Meloidogyne, Pratylenchus, and other nematode species tested. Polymerase chain reaction (PCR) and amplification conditions were established, and H. avenae and H. filipjevi were clearly distinguished by PCR fragments of 242 and 170 bp, respectively. Robust PCR amplification was achieved with DNA extracted from a single egg or second-stage juvenile (J2) using a laboratory-made worm lysis buffer, and DNA from 0.5 egg or J2 using a commercial kit. The PCR assays were successfully employed for differentiation of H. filipjevi and H. avenae populations collected from eight locations in three Pacific Northwest states. This is the first report of a species-specific ITS PCR assay to detect and identify H. filipjevi. The assays for both species will enhance diagnosis of cereal cyst nematode species in infested fields.

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PCR SURVEILLANCE OF FOUR PROTOTYPIC RAT PARVOVIRUSES IN LABORATORY RAT COLONIES IN TAIWAN

Taiwan Veterinary Journal ◽

10.1142/s1682648520500018 ◽

2020 ◽

Vol 46 (01) ◽

pp. 29-35 ◽

Cited By ~ 1

Author(s):

Yung-Hui Chang ◽

Yi-Chun Liao ◽

Ko-Wei Wang ◽

Ming-Hseng Wang ◽

Ber-Hsiung Fang ◽

...

Keyword(s):

Monitoring Program ◽

Dual Infection ◽

Virus Species ◽

Laboratory Rats ◽

Specific Pcr ◽

Laboratory Rat ◽

Minute Virus ◽

Pcr Assays ◽

Species Specific ◽

Infectious Condition

Prototypic rat parvoviruses are among the most common infectious agents in laboratory rats worldwide. It is important to determine the infectious condition of rat parvoviruses in research colonies in Taiwan. In this study, virus species-specific PCR assays were applied to screen Kilham rat virus (KRV), Toolan’s H-1 virus (H-1 virus), rat parvovirus (RPV) and rat minute virus (RMV) in 203 rats from five different research colonies in Taiwan. In this survey, the infection rate of rat parvoviruses was 19.2% (39/203), including KRV (12.3%; 25/203), RMV (8.9%; 18/203) and RPV (1.0%; 2/203). Dual infection of KRV and RMV were detected in 3.0% (6/203) of samples. No rats were positive for H-1 virus. The infectious rates of KRV and RMV detected in this study are much higher than those reported in USA and in Europe. Rat parvovirus PCR assays reported in this paper could be a useful tool for routine rat health monitoring program applied in laboratory rat facilities. [Chang YH, Liao YC, Wang KW, Hsieh SC, Wang MH, Fang BH, Chang CY, Chueh LL, Wan CH, PCR Surveillance of four prototypic rat parvoviruses in laboratory rat colonies in Taiwan, Taiwan Vet J 46(1): 1–7, 2020]

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Species-specific PCR Assays for the Fungal Pathogens Fusarium moniliforme and Fusarium subglutinans and their Application to Diagnose Maize Ear Rot Disease

Journal of Phytopathology ◽

10.1111/j.1439-0434.1999.tb03856.x ◽

1999 ◽

Vol 147 (9) ◽

pp. 497-508 ◽

Cited By ~ 44

Author(s):

E. M. Möller ◽

J. Chełkowski ◽

H. H. Geiger

Keyword(s):

Fusarium Moniliforme ◽

Fungal Pathogens ◽

Ear Rot ◽

Fusarium Subglutinans ◽

Specific Pcr ◽

Pcr Assays ◽

Rot Disease ◽

Species Specific ◽

Maize Ear Rot ◽

Maize Ear

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Authentication of a traditional game meat sausage (Alheira) by species-specific PCR assays to detect hare, rabbit, red deer, pork and cow meats

Food Research International ◽

10.1016/j.foodres.2013.11.003 ◽

2014 ◽

Vol 60 ◽

pp. 140-145 ◽

Cited By ~ 38

Author(s):

Joana S. Amaral ◽

Cristina G. Santos ◽

Vitor S. Melo ◽

M. Beatriz P.P. Oliveira ◽

Isabel Mafra

Keyword(s):

Red Deer ◽

Game Meat ◽

Specific Pcr ◽

Pcr Assays ◽

Species Specific ◽

Traditional Game

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Identification of Seven Species of the Genus Cronobacter and Discrimination of a False-Positive Bacterium in ISO/TS 22964 by Genus- and Species-Specific PCR Assays

Japanese Journal of Food Microbiology ◽

10.5803/jsfm.33.194 ◽

2016 ◽

Vol 33 (4) ◽

pp. 194-201

Author(s):

Masaharu Nishihara ◽

Tomoko Sudo ◽

Mari Miyamoto ◽

Satoshi Ishii ◽

Shuichi Yanahira ◽

...

Keyword(s):

False Positive ◽

Positive Bacterium ◽

Specific Pcr ◽

Pcr Assays ◽

Species Specific

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Rapid detection and identification of Streptococcus ratti by a species-specific PCR method

Anaerobe ◽

10.1016/j.anaerobe.2011.09.001 ◽

2012 ◽

Vol 18 (1) ◽

pp. 44-47

Author(s):

Jumpei Nishio ◽

Makoto Taniguchi ◽

Juichiro Higashi ◽

Masakazu Takahashi ◽

Takuma Ando ◽

...

Keyword(s):

Rapid Detection ◽

Specific Pcr ◽

Detection And Identification ◽

Pcr Method ◽

Species Specific ◽

Streptococcus Ratti

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Genus- and Species-Specific PCR-Based Detection of Dairy Propionibacteria in Environmental Samples by Using Primers Targeted to the Genes Encoding 16S rRNA

Applied and Environmental Microbiology ◽

10.1128/aem.65.9.4241-4244.1999 ◽

1999 ◽

Vol 65 (9) ◽

pp. 4241-4244 ◽

Cited By ~ 34

Author(s):

Franca Rossi ◽

Sandra Torriani ◽

Franco Dellaglio

Keyword(s):

16S Rrna ◽

Environmental Samples ◽

Specific Pcr ◽

Genes Encoding ◽

Propionibacterium Thoenii ◽

Pcr Assays ◽

Dairy Propionibacteria ◽

Species Specific

ABSTRACT PCR assays with primers targeted to the genes encoding 16S rRNA were developed for detection of dairy propionibacteria.Propionibacterium thoenii specific oligonucleotide PT3 was selected after partial resequencing. Tests allowed the detection of less than 10 cells per reaction from milk and cheese and 102 cells per reaction from forage and soil.

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