scholarly journals Molecular genetic and clinical aspects of socially relevant viruses underlying congenital diseases

2021 ◽  
Vol 11 (4) ◽  
pp. 635-648
Author(s):  
V. V. Vasilev ◽  
N. V. Rogozina ◽  
A. A. Grineva
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Infectious Diseases ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Molecular Genetic ◽  
Clinical Manifestations ◽  
Clinical Aspects ◽  
Herpes Simplex Viruses ◽  
Simplex Virus

Congenital viral infectious diseases are characterized by polyetiologic pathology holding an important place in the structure of perinatal losses. Due to the wide distribution and lack of specific prophylaxis, the problem of herpesvirus infections is of greatest interest, namely of herpes infection caused by herpes simplex virus type 1 and 2, human herpes simplex virus type 6 and cytomegalovirus infection, as well as parvovirus infection B19. The opportunities to investigate a relation between manifestations of the infectious process and host molecular genetic characteristics have been expanded after developing full genome sequencing methods and creating genetic data international banks. It has been proven that herpes virus genetic variations can account for related neurovirulence, showing that diverse cytomegalovirus genotypes are associated with hepatosplenomegaly, hearing impairment and the symptoms of the central nervous system diseases. Nevertheless, the data on correlation between genotypes and clinical manifestations are still scarce and contradictory, whereas high level of variability becomes extremely evident while comparing genomic sequences of viral strains. The herpesvirus type 6 has been proven to integrate into germ cells with potential for subsequent vertical transmission of chromosomally integrated virus to the offspring and its further intergeneration inheritance. А direct relationship between B19V genospecies and disease manifestations including congenital infections has not yet been identified. Taking into account possible differences in the geographical distribution of such viruses on the territory of the Russian Federation, ethnic populational characteristics, and high frequency of related congenital infectious diseases with a wide range of clinical manifestations, it seems promising to expand scientific research on the genotyping of herpes simplex viruses, cytomegalovirus, herpes viruses type 6 and parvovirus B19V in Russia. The results of such studies will be demanded by practical healthcare in order to develop and use more effective etiotropic drugs and specific prophylaxis in the light of trends to develop personalized and preventive medicine.

scholarly journals An epidemiologic study of herpes simplex virus type 1 and 2 infection in Japan based on type-specific serological assays

1998 ◽  
Vol 120 (2) ◽  
pp. 179-186 ◽  
Author(s):  
M. HASHIDO ◽  
F. K. LEE ◽  
A. J. NAHMIAS ◽  
H. TSUGAMI ◽  
S. ISOMURA ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Pregnant Women ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Epidemiologic Study ◽  
Clinical Manifestations ◽  
Simplex Virus ◽  
Hsv 1

A seroepidemiologic study of herpes simplex virus type 1 (HSV-1) and 2 (HSV-2) was performed on Japanese adults. Serum samples collected between 1985–9 from a total of 536 healthy adults, female prostitutes, males with sexually transmitted diseases (STD), homosexual men, and pregnant women were studied by immunodot assays using HSV type-specific antigens, glycoproteins G (gG1 and gG2). HSV-1 infections correlated mostly with age and was widely prevalent among subjects <40 years. HSV-2 prevalence varied greatly among subgroups defined by sexual activity and was associated with risk behaviours for prostitution, infection with STD, and homosexual activity. HSV-2 seroprevalence was highest among prostitutes (80%), lowest among pregnant women (7 %), and intermediate in STD patients (23%) and homosexuals (24%). Because HSV-1 infection during childhood has been decreasing, primary genital HSV-2 infection, with its higher frequency of clinical manifestations, will become a greater burden to the public health in Japan.

Characterization of mutants of herpes simplex viruses, types 1 and 2, that produce fragments of the thymidine kinase polypeptide

1983 ◽  
Vol 29 (4) ◽  
pp. 385-393
Author(s):  
Timothy M. -P. Block ◽  
Nancy J. Kuhn ◽  
Karen A. Kustas ◽  
William A. Held ◽  
Kenneth Gross ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
In Vitro Translation ◽  
Herpes Simplex Viruses ◽  
Simplex Virus ◽  
Hsv 1

Seven tk− mutants of herpes simplex virus, type 2 (HSV-2), and three tk− mutants of herpes simplex virus, type 1 (HSV-1), were isolated which did not produce the thymidine kinase (TK) polypeptides but formed smaller polypeptides not seen in wild-type infected cells. Positive TK mRNA selection by hybridization to the cloned tk genes followed by in vitro translation identified the TK polypeptides. Comparisons of the products of partial proteolysis of the polypeptides of four HSV-2 and two HSV-1 tk− mutants to those of the parental TK polypeptides indicated that, in each case, the novel polypeptide was a fragment of the TK polypeptide, showing that these mutants have defects in the structural gene for tk. HSV-2 mutants of this sort have not been previously described. They and the HSV-1 mutants are similar to HSV-1 mutants reported previously. In addition, it was found that TK mRNA was present early in infection but was absent late in infection, suggesting that the shutoff of TK synthesis is due to message degradation. Also, HSV-2 TK mRNA did not hybridize to the cloned HSV-1 tk gene indicating that these genes have extensively diverged.

Molecular genetic analysis of thymidine kinase of the herpes simplex virus type 1

2005 ◽  
Vol 39 (1) ◽  
pp. 137-140 ◽  
Author(s):  
A. A. Guskova ◽  
A. V. Zagurny ◽  
M. Yu. Skoblov ◽  
A. V. Baranova ◽  
V. L. Andronova ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Genetic Analysis ◽  
Thymidine Kinase ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Simplex Virus

Prophylactic Oral Acyclovir in Outbreaks of Primary Herpes Simplex Virus Type 1 Infection in a Closed Community

PEDIATRICS ◽  
1992 ◽  
Vol 89 (3) ◽  
pp. 379-383
Author(s):  
Kiyotaka Kuzushima ◽  
Toyoichiro Kudo ◽  
Hiroshi Kimura ◽  
Shinji Kido ◽  
Naoki Hanada ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Clinical Manifestations ◽  
Oral Acyclovir ◽  
Symptomatic Disease ◽  
Control Subjects ◽  
Simplex Virus

Oral acyclovir was given prophylactically to 37 children in the early stages of three outbreaks of herpes simplex virus type 1 (HSV-1) infection and the results were compared with those in untreated control subjects in two other outbreaks. The rates of seroconversion to HSV were significantly reduced in children treated with acyclovir compared with control subjects (91% vs 27%, P &lt; .001). The incidence of symptomatic disease was also significantly reduced (82% vs 0%, P &lt; .001). In some children receiving prophylactic acyclovir, anti-HSV antibody titers did not rise despite the presence of replicative HSV on throat swabs just before the start of treatment. Restriction endonuclease analysis of isolated HSV-DNA confirmed that one strain was responsible for the five outbreaks. No resistance to acyclovir was detected during the study, and no adverse effects of treatment were noted. In conclusion, short-term prophylactic acyclovir may limit the spread and reduce clinical manifestations of HSV infections in closed communities, although this use should be restricted to communities where severe symptoms are observed.

Role of herpes simplex virus type 1 (HSV-1) in the pathogenesis of peptic ulcer disease

2001 ◽  
Vol 120 (5) ◽  
pp. A136-A137
Author(s):  
K TSAMAKIDES ◽  
E PANOTOPOULOU ◽  
D DIMITROULOPOULOS ◽  
M CHRISTOPOULO ◽  
D XINOPOULOS ◽  
...  
Keyword(s):  
Peptic Ulcer ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Peptic Ulcer Disease ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Ulcer Disease ◽  
Simplex Virus
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