Serological responses in sheep injected with plasmids encoding bovine herpesvirus 1 (BHV-1) gD glycoprotein

A genetic vaccine consisting of the bovine herpesvirus-1.2a (BHV-1.2a) glycoprotein D (gD) gene under the control of the cytomegalovirus immediate-early promoter/enhancer was generated and administered to sheep intramuscularly in the neck. All animals developed serum antibodies which recognized the homologous antigen (BHV-1.2a strain BH-83) and also exhibited cross-reactivity against the heterologous antigen (BHV-5 strain EVI-190). Three intramuscularly injections were given but serological responses were not improved after the second inoculation. Specific antibodies were detected against BHV-1.2a until at least 12 months after the first inoculation. However, the capacity to induce antibodies against BHV-5 was lower and of shorter duration than to BHV-1.2a.

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Modulation of immune responses to bovine herpesvirus-1 in cattle by immunization with a DNA vaccine encoding glycoprotein D as a fusion protein with bovine CD154

Immunology ◽

10.1111/j.1365-2567.2004.01877.x ◽

2004 ◽

Vol 112 (2) ◽

pp. 328-338 ◽

Cited By ~ 27

Author(s):

Sharmila Manoj ◽

Philip J. Griebel ◽

Lorne A. Babiuk ◽

Sylvia Van Drunen Littel-Van Den Hurk

Keyword(s):

Immune Responses ◽

Fusion Protein ◽

Dna Vaccine ◽

Bovine Herpesvirus ◽

Glycoprotein D ◽

Bovine Herpesvirus 1 ◽

Herpesvirus 1

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Bovine herpesvirus 1 glycoprotein D expression in bovine upper respiratory tract mediated by a human adenovirus type 5

Veterinary Research ◽

10.1051/vetres:2004045 ◽

2004 ◽

Vol 35 (6) ◽

pp. 715-721 ◽

Cited By ~ 6

Author(s):

Sacha Gogev ◽

Jean-Pierre Georgin ◽

Fr�d�ric Schynts ◽

Alain Vanderplasschen ◽

Etienne Thiry

Keyword(s):

Respiratory Tract ◽

Bovine Herpesvirus ◽

Human Adenovirus ◽

Adenovirus Type ◽

Upper Respiratory Tract ◽

Glycoprotein D ◽

Bovine Herpesvirus 1 ◽

Upper Respiratory ◽

Herpesvirus 1 ◽

Adenovirus Type 5

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Genetic diversity of 3′ region of glycoprotein D gene of bovine herpesvirus 1 and 5

Virus Genes ◽

10.1007/s11262-014-1040-5 ◽

2014 ◽

Vol 48 (3) ◽

pp. 438-447 ◽

Cited By ~ 7

Author(s):

Carolina Kist Traesel ◽

Mariana Sá e Silva ◽

Marcelo Weiss ◽

Fernando Rosado Spilki ◽

Rudi Weiblen ◽

...

Keyword(s):

Genetic Diversity ◽

Bovine Herpesvirus ◽

Glycoprotein D ◽

Bovine Herpesvirus 1 ◽

Herpesvirus 1

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Crystal structure of bovine herpesvirus 1 glycoprotein D bound to nectin-1 reveals the basis for its low-affinity binding to the receptor

Science Advances ◽

10.1126/sciadv.aba5147 ◽

2020 ◽

Vol 6 (20) ◽

pp. eaba5147

Author(s):

Dan Yue ◽

Zhujun Chen ◽

Fanli Yang ◽

Fei Ye ◽

Sheng Lin ◽

...

Keyword(s):

Functional Data ◽

Bovine Herpesvirus ◽

Binding Mode ◽

Cell Entry ◽

Glycoprotein D ◽

Viral Glycoprotein ◽

Bovine Herpesvirus 1 ◽

Receptor Recognition ◽

Large Conformational Change ◽

Herpesvirus 1

Bovine herpesvirus 1 (BHV-1) has received increasing attention for its potential oncolytic applications. BHV-1 recognizes nectin-1 for cell entry via viral glycoprotein D (gD) but represents a low-affinity nectin-1 binding virus. The molecular basis underlying this low receptor-binding affinity, however, remains unknown. Here, the crystal structures of BHV-1 gD in the free and nectin-1–bound forms are presented. While showing an overall resembled nectin-1 binding mode to other alphaherpesvirus gDs, BHV-1 gD has a unique G-strand/α2-helix interloop that disturbs gD/nectin-1 interactions. Residue R188 residing in this loop is observed to otherwise cause strong steric hindrance with the bound receptor, making a large conformational change of the loop a prerequisite for nectin-1 engagement. Subsequently, substitution of R188 with glycine markedly enhances the affinity of the BHV-1-gD/nectin-1 interaction (by about fivefold). These structural and functional data delineate the receptor-recognition basis for BHV-1, which might facilitate BHV-1–based oncolytic design in the future.

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