scholarly journals Optimization of Recombinant Glycoprotein D (gD) based Indirect ELISA for Detection of Antibodies against Bovine Herpesvirus-1

2020 ◽  
Vol 8 (8) ◽  
Author(s):  
Barkha Ratta ◽  
Swagatika Priyadarsini ◽  
Nikhil K. Channabasappa ◽  
Pashupathi Mani ◽  
Meeta Saxena ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Bovine Herpesvirus ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Recombinant Glycoprotein ◽  
Herpesvirus 1

scholarly journals Bovine herpesvirus 1 glycoprotein D expression in bovine upper respiratory tract mediated by a human adenovirus type 5

2004 ◽  
Vol 35 (6) ◽  
pp. 715-721 ◽  
Author(s):  
Sacha Gogev ◽  
Jean-Pierre Georgin ◽  
Fr�d�ric Schynts ◽  
Alain Vanderplasschen ◽  
Etienne Thiry
Keyword(s):  
Respiratory Tract ◽  
Bovine Herpesvirus ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Upper Respiratory Tract ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Upper Respiratory ◽  
Herpesvirus 1 ◽  
Adenovirus Type 5

Genetic diversity of 3′ region of glycoprotein D gene of bovine herpesvirus 1 and 5

Virus Genes ◽  
2014 ◽  
Vol 48 (3) ◽  
pp. 438-447 ◽  
Author(s):  
Carolina Kist Traesel ◽  
Mariana Sá e Silva ◽  
Marcelo Weiss ◽  
Fernando Rosado Spilki ◽  
Rudi Weiblen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Bovine Herpesvirus ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

scholarly journals Crystal structure of bovine herpesvirus 1 glycoprotein D bound to nectin-1 reveals the basis for its low-affinity binding to the receptor

2020 ◽  
Vol 6 (20) ◽  
pp. eaba5147
Author(s):  
Dan Yue ◽  
Zhujun Chen ◽  
Fanli Yang ◽  
Fei Ye ◽  
Sheng Lin ◽  
...  
Keyword(s):  
Functional Data ◽  
Bovine Herpesvirus ◽  
Binding Mode ◽  
Cell Entry ◽  
Glycoprotein D ◽  
Viral Glycoprotein ◽  
Bovine Herpesvirus 1 ◽  
Receptor Recognition ◽  
Large Conformational Change ◽  
Herpesvirus 1

Bovine herpesvirus 1 (BHV-1) has received increasing attention for its potential oncolytic applications. BHV-1 recognizes nectin-1 for cell entry via viral glycoprotein D (gD) but represents a low-affinity nectin-1 binding virus. The molecular basis underlying this low receptor-binding affinity, however, remains unknown. Here, the crystal structures of BHV-1 gD in the free and nectin-1–bound forms are presented. While showing an overall resembled nectin-1 binding mode to other alphaherpesvirus gDs, BHV-1 gD has a unique G-strand/α2-helix interloop that disturbs gD/nectin-1 interactions. Residue R188 residing in this loop is observed to otherwise cause strong steric hindrance with the bound receptor, making a large conformational change of the loop a prerequisite for nectin-1 engagement. Subsequently, substitution of R188 with glycine markedly enhances the affinity of the BHV-1-gD/nectin-1 interaction (by about fivefold). These structural and functional data delineate the receptor-recognition basis for BHV-1, which might facilitate BHV-1–based oncolytic design in the future.

An Immunoenzyme Linked Assay (ELISA) for the Detection of Antibodies to Truncated Glycoprotein D (tgD) of Bovine Herpesvirus-1

2006 ◽  
Vol 30 (S1) ◽  
pp. 257-259 ◽  
Author(s):  
S. Petrini ◽  
M. Ferrari ◽  
S. Vincenzetti ◽  
A. Vita ◽  
A. Amici ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

scholarly journals Transcription Map and Expression of Bovine Herpesvirus-1 Glycoprotein D in Early Region 4 of Bovine Adenovirus-3

Virology ◽  
1999 ◽  
Vol 261 (1) ◽  
pp. 143-152 ◽  
Author(s):  
Mohit K. Baxi ◽  
Lorne A. Babiuk ◽  
Majid Mehtali ◽  
Suresh K. Tikoo
Keyword(s):  
Bovine Herpesvirus ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Bovine Adenovirus ◽  
Early Region ◽  
Herpesvirus 1 ◽  
Early Region 4 ◽  
Transcription Map

scholarly journals Bovine Herpesvirus 1-Induced Apoptotic Cell Death: Role of Glycoprotein D

Virology ◽  
1999 ◽  
Vol 257 (1) ◽  
pp. 191-197 ◽  
Author(s):  
Emmanuel Hanon ◽  
Günther Keil ◽  
Sylvia van Drunen Littel-van den Hurk ◽  
Philip Griebel ◽  
Alain Vanderplasschen ◽  
...  
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Bovine Herpesvirus ◽  
Apoptotic Cell Death ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

scholarly journals The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides

2002 ◽  
Vol 76 (18) ◽  
pp. 9002-9010 ◽  
Author(s):  
X. P. Ioannou ◽  
P. Griebel ◽  
R. Hecker ◽  
L. A. Babiuk ◽  
S. van Drunen Littel-van den Hurk
Keyword(s):  
Immune Response ◽  
Neutralizing Antibodies ◽  
Protective Efficacy ◽  
Bovine Herpesvirus ◽  
Ammonium Bromide ◽  
Cpg Odn ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Cpg Oligodeoxynucleotides ◽  
Herpesvirus 1

ABSTRACT The immunogenicity and protective efficacy of a bovine herpesvirus 1 (BHV-1) subunit vaccine formulated with Emulsigen (Em) and a synthetic oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) was determined in cattle. A truncated, secreted version of BHV-1 glycoprotein D (tgD) formulated with Em and CpG ODN at concentrations of 25, 2.5, or 0.25 mg/dose produced a more balanced immune response, higher levels of virus neutralizing antibodies, and greater protection after BHV-1 challenge compared to tgD adjuvanted with either Em or CpG ODN alone. In contrast, tgD formulated with Em and either 25 mg of a non-CpG ODN or another immunostimulatory compound, dimethyl dioctadecyl ammonium bromide, induced similar immunity and protection compared to tgD formulated with Em alone, a finding which confirms the immunostimulatory effect of ODN to be CpG motif mediated. Our results demonstrate the ability of CpG ODN to induce a strong and balanced immune response in a target species.

Sign in / Sign up

Export Citation Format

Share Document