scholarly journals Citotoxicidade de plantas com indicativo etnográfico para a desinfecção de água

2009 ◽  
Vol 11 (3) ◽  
pp. 305-309 ◽  
Author(s):  
A.R. Gonçalves ◽  
J.M. Wiest ◽  
P.M. Roehe ◽  
H.H. Carvalho
Keyword(s):  
Sus Scrofa ◽  
Chenopodium Album ◽  
Canis Familiaris ◽  
African Green Monkey ◽  
Kidney Cells ◽  
Green Monkey ◽  
African Green Monkey Cells ◽  
Bryophyllum Pinnatum

A água para o consumo humano e animal é um patrimônio cada vez mais escasso que é necessário preservar. O processo de desinfecção utilizado atualmente tem como base desinfetantes clorados, que em combinação com resíduos orgânicos, resultam em trihalometanos (potencialmente oncogênicos). A proposta deste trabalho foi avaliar a citotoxicidade de cinco extratos de plantas, capazes de promover desinfecção da água, frente a seis linhagens celulares, a saber: Vero (ATCC CCl81) African green monkey cells; MDBK (ATCC CCL 24) Martin Darby Bonne Kidney cells; MDCK (ATCC CCL 34) Canis familiaris kidney cells; CRFK (ATCC CCL 94) Felix catus kidney cells; PK 15 (ATCC CCL 33) Sus scrofa kidney cells; RK13 (ATCC CCL 37) Orytolagus cuniculus Kidney cells, e avaliar cultivos celulares mais sensíveis para o teste de toxicidade de extratos de plantas. Destes extratos, o mais tóxico foi o de Erva de Formigueiro (Chenopodium álbum) diante de todas as células testadas. O extrato de chapéu de couro (Sagittaria montevidensis) foi medianamente tóxico e os demais baleeira (Cordia curassavica), folha da fortuna (Bryophyllum pinnatum [Kurz]) e sete sangrias (Cuphea carthagenensis [Jacq.] J.F. Macbride) foram os menos tóxicos. Este resultado foi estatisticamente significativo (p=0,99), quando se comparou o extrato das plantas frente às concentrações e às células estudadas. Entre as células utilizadas, as mais sensíveis foram a VERO (rim de macaco) e a CRFK (rim de gato) quando comparadas com as demais foram (MDBK, MDCK, PK 15, RK 13), com diferença significativa (p=0,99). Conclui-se que há necessidade de serem avaliados os extratos vegetais quanto a toxicidade, antes do emprego como recursos de saúde, e que algumas células são mais eficazes que outras na detecção de efeito citopatogênico.

Surface expression of influenza virus neuraminidase, an amino-terminally anchored viral membrane glycoprotein, in polarized epithelial cells

1985 ◽  
Vol 5 (9) ◽  
pp. 2181-2189
Author(s):  
L V Jones ◽  
R W Compans ◽  
A R Davis ◽  
T J Bos ◽  
D P Nayak
Keyword(s):  
Influenza Virus ◽  
Influenza A ◽  
Surface Expression ◽  
African Green Monkey ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
African Green Monkey Kidney ◽  
Amino Terminal ◽  
Green Monkey ◽  
Na Gene

We have investigated the site of surface expression of the neuraminidase (NA) glycoprotein of influenza A virus, which, in contrast to the hemagglutinin, is bound to membranes by hydrophobic residues near the NH2-terminus. Madin-Darby canine kidney or primary African green monkey kidney cells infected with influenza A/WSN/33 virus and subsequently labeled with monoclonal antibody to the NA and then with a colloidal gold- or ferritin-conjugated second antibody exhibited specific labeling of apical surfaces. Using simian virus 40 late expression vectors, we also studied the surface expression of the complete NA gene (SNC) and a truncated NA gene (SN10) in either primary or a polarized continuous line (MA104) of African green monkey kidney cells. The polypeptides encoded by the cloned NA cDNAs were expressed on the surface of both cell types. Analysis of [3H]mannose-labeled polypeptides from recombinant virus-infected MA104 cells showed that the products of cloned NA cDNA comigrated with glycosylated NA from influenza virus-infected cells. Both the complete and the truncated glycoproteins were found to be preferentially expressed on apical plasma membranes, as detected by immunogold labeling. These results indicate that the NA polypeptide contains structural features capable of directing the transport of the protein to apical cell surfaces and the first 10 amino-terminal residues of the NA polypeptide are not involved in this process.

Assessment of potential miRNA biomarkers of VERO-cell tumorigenicity in a new line (AGMK1-9T7) of African green monkey kidney cells

Vaccine ◽  
2017 ◽  
Vol 35 (41) ◽  
pp. 5503-5509 ◽  
Author(s):  
Belete Teferedegne ◽  
Daniel M. Rotroff ◽  
Juliete Macauley ◽  
Gideon Foseh ◽  
Gladys Lewis ◽  
...  
Keyword(s):  
Vero Cell ◽  
African Green Monkey ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
African Green Monkey Kidney ◽  
Green Monkey

scholarly journals UV Irradiation induces an activity which stimulates Simian virus 40 rescue upon cell fusion.

1984 ◽  
Vol 4 (6) ◽  
pp. 1159-1162 ◽  
Author(s):  
S Nomura ◽  
M Oishi
Keyword(s):  
Cell Fusion ◽  
Uv Irradiation ◽  
Syrian Hamster ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
African Green Monkey ◽  
Green Monkey ◽  
African Green Monkey Cells ◽  
Stimulation Of

UV irradiation of African green monkey cells greatly stimulated efficiency of simian virus 40 induction from simian virus 40-transformed Syrian hamster cells after cell fusion. The maximum inducing activity was observed at 15 to 20 h after irradiation but remained only transiently. The addition of cycloheximide after UV irradiation eliminated the stimulation of the activity.

scholarly journals Surface expression of influenza virus neuraminidase, an amino-terminally anchored viral membrane glycoprotein, in polarized epithelial cells.

1985 ◽  
Vol 5 (9) ◽  
pp. 2181-2189 ◽  
Author(s):  
L V Jones ◽  
R W Compans ◽  
A R Davis ◽  
T J Bos ◽  
D P Nayak
Keyword(s):  
Influenza Virus ◽  
Influenza A ◽  
Surface Expression ◽  
African Green Monkey ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
African Green Monkey Kidney ◽  
Amino Terminal ◽  
Green Monkey ◽  
Na Gene

We have investigated the site of surface expression of the neuraminidase (NA) glycoprotein of influenza A virus, which, in contrast to the hemagglutinin, is bound to membranes by hydrophobic residues near the NH2-terminus. Madin-Darby canine kidney or primary African green monkey kidney cells infected with influenza A/WSN/33 virus and subsequently labeled with monoclonal antibody to the NA and then with a colloidal gold- or ferritin-conjugated second antibody exhibited specific labeling of apical surfaces. Using simian virus 40 late expression vectors, we also studied the surface expression of the complete NA gene (SNC) and a truncated NA gene (SN10) in either primary or a polarized continuous line (MA104) of African green monkey kidney cells. The polypeptides encoded by the cloned NA cDNAs were expressed on the surface of both cell types. Analysis of [3H]mannose-labeled polypeptides from recombinant virus-infected MA104 cells showed that the products of cloned NA cDNA comigrated with glycosylated NA from influenza virus-infected cells. Both the complete and the truncated glycoproteins were found to be preferentially expressed on apical plasma membranes, as detected by immunogold labeling. These results indicate that the NA polypeptide contains structural features capable of directing the transport of the protein to apical cell surfaces and the first 10 amino-terminal residues of the NA polypeptide are not involved in this process.

UV Irradiation induces an activity which stimulates Simian virus 40 rescue upon cell fusion

1984 ◽  
Vol 4 (6) ◽  
pp. 1159-1162
Author(s):  
S Nomura ◽  
M Oishi
Keyword(s):  
Cell Fusion ◽  
Uv Irradiation ◽  
Syrian Hamster ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
African Green Monkey ◽  
Green Monkey ◽  
African Green Monkey Cells ◽  
Stimulation Of

UV irradiation of African green monkey cells greatly stimulated efficiency of simian virus 40 induction from simian virus 40-transformed Syrian hamster cells after cell fusion. The maximum inducing activity was observed at 15 to 20 h after irradiation but remained only transiently. The addition of cycloheximide after UV irradiation eliminated the stimulation of the activity.

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