This study presents a numerical simulation of a 3D viscous subsonic flow in the VKI-Genoa turbine cascade taking into account the laminar-turbulent transition. The numerical simulation is performed using the Reynolds-averaged Navier-Stokes (RANS) equations and the low-Reynolds k-ω SST turbulence model. The Langtry’s algebraic Production Term Modification (PTM) model is applied for modeling the laminar-turbulent transition. The governing equations are integrated using the second-order accurate Godunov’s type implicit ENO scheme. Computations of both fully turbulent and transitional flows are carried out. Much attention is given to the comparison between the present numerical results and the existing experimental data. The comparison was based on the surface distributions of the isentropic velocity, the friction velocity, the flow acceleration parameter, the displacement thickness, the shape-factor, and the momentum thickness Reynolds number. Velocity profiles upstream and downstream of the transition onset were compared also. The numerical results obtained show an influence of the transition on the secondary flow pattern. In the case of the transitional flow, when compared with the fully turbulence flow case, the endwall boundary layer cross-flow starts upstream, and it is more intensive, but less massive due to a thinner boundary layer in the laminar flow region.