scholarly journals Studies on porcine pancreatic elastase activity. II. Immunoreactive elastase level during acute hemorrhagic pancreatitis in pigs.

1980 ◽  
Vol 131 (2) ◽  
pp. 127-134 ◽  
Author(s):  
YASUYUKI NAKAJIMA ◽  
SEIKI MATSUNO ◽  
NOBORU NOTO ◽  
YOICHI SAITOH ◽  
TOSHIO SATO
Keyword(s):  
Elastase Activity ◽  
Pancreatic Elastase ◽  
Hemorrhagic Pancreatitis ◽  
Porcine Pancreatic Elastase ◽  
Elastase Level ◽  
Acute Hemorrhagic Pancreatitis ◽  
Immunoreactive Elastase

scholarly journals Adsorbed soluble [3H]elastin as substrate for proteinase activity. A new microassay technique

1984 ◽  
Vol 218 (2) ◽  
pp. 645-648 ◽  
Author(s):  
H S Jensen
Keyword(s):  
Coefficient Of Variation ◽  
Proteinase Activity ◽  
Elastase Activity ◽  
Pancreatic Elastase ◽  
Passive Adsorption ◽  
Interassay Coefficient ◽  
Scintillation Fluid ◽  
Porcine Pancreatic Elastase

A microassay for elastase activity has been designed. Microtubes are coated with soluble elastin substrate by passive adsorption to the tube walls. On contact with enzymes in test samples, radioactivity is released into the sample buffer, which is then transferred to vials containing scintillation fluid without further processing. Porcine pancreatic elastase (EC 3.4.21.11) is easily detected in concentrations down to 8 micrograms/litre. The interassay coefficient of variation (CV) was below 10% for values above 50 micrograms/litre. The assay is rapid, precise, economical and sensitive.

scholarly journals Studies on porcine pancreatic elastase activity. I. Radioimmuoassay of porcine pancreatic elastase.

1980 ◽  
Vol 131 (2) ◽  
pp. 119-126 ◽  
Author(s):  
YASUYUKI NAKAJIMA ◽  
SEIKI MATSUNO ◽  
NOBORU NOTO ◽  
YOICHI SAITOR ◽  
TOSHIO SATO
Keyword(s):  
Elastase Activity ◽  
Pancreatic Elastase ◽  
Porcine Pancreatic Elastase

THE SELECTIVE PROTEOLYTIC ENZYME INHIBITORY ACTION OF BENZETHONIUM CHLORIDE

1960 ◽  
Vol 38 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Ivan T. Beck ◽  
E. Pinter ◽  
R. D. McKenna ◽  
H. Griff
Keyword(s):  
Subcutaneous Injection ◽  
Proteolytic Enzyme ◽  
Inhibitory Action ◽  
Hemorrhagic Pancreatitis ◽  
Benzethonium Chloride ◽  
Tryptic Activity ◽  
High Concentrations ◽  
Acute Hemorrhagic Pancreatitis ◽  
Very High

Acute hemorrhagic pancreatitis in humans is thought to be perpetuated by the autolytic processes catalyzed by trypsin and lipase. This study is an integral part of our search for trypsin and lipase inhibitors to be used in the treatment of this disease.Benzethonium chloride was found to inhibit tryptic activity in vitro. The proteolytic activity of rabbit's serum was inhibited, and the inhibition was most pronounced 6 to 12 hours after the subcutaneous injection of the compound. Fibrinolysin was also inhibited in vitro but benzethonium chloride had no inhibitory action on chymotrypsin, pepsin, or lipase.Serum proteins in vitro were precipitated only with very high concentrations of the compound. No significant protein changes were observed in sera of rabbits after the subcutaneous injection of the compound.

scholarly journals Acute Hemorrhagic Pancreatitis and Pseudocyst Due to Mumps

1974 ◽  
Vol 180 (1) ◽  
pp. 85-88 ◽  
Author(s):  
JEFFREY D. FELDSTEIN ◽  
FRANK R. JOHNSON ◽  
CHARLES A. KALLICK ◽  
S ALEXANDER DOOLAS
Keyword(s):  
Hemorrhagic Pancreatitis ◽  
Acute Hemorrhagic Pancreatitis

The Effects of Various Lipids upon the Rate of Elastolysis of Bovine Ligamentum Nuchae Elastin by Porcine Pancreatic Elastase

1985 ◽  
Vol 68 (s11) ◽  
pp. 26P-27P
Author(s):  
S A Wharton ◽  
G J Phillips ◽  
Jmdc Pereira ◽  
Dcs Hutchison ◽  
H Baum
Keyword(s):  
Pancreatic Elastase ◽  
Porcine Pancreatic Elastase
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