ONTOGENETIC AND PHYLOGENETIC DISTRIBUTION OF HYDROXYSTEROID DEHYDROGENASES IN THE VERTEBRATE KIDNEY

1966 ◽  
Vol 36 (1) ◽  
pp. 29-NP ◽  
Author(s):  
A. H. BAILLIE ◽  
M. M. FERGUSON ◽  
D. McK. HART
Keyword(s):  
Dehydrogenase Activity ◽  
Sex Steroids ◽  
Hydroxysteroid Dehydrogenase ◽  
Phylogenetic Distribution ◽  
Hydroxysteroid Dehydrogenases ◽  
Collecting Tubules ◽  
Convoluted Tubules

SUMMARY The human pronephros showed no hydroxysteroid dehydrogenase activity. The human mesonephros, like piscine and amphibian mesonephroi had 16β- and 17β-hydroxysteroid dehydrogenase activity and a possible function of the human mesonephros is suggested. Metanephric kidneys had 3α-, Δ5-3β-, 3β-, 6β-, 16α-, 16β-, and 17β-hydroxysteroid dehydrogenases; 11β-hydroxysteroid dehydrogenase was present in all adult mammalian metanephric kidneys surveyed. 3α-Hydroxysteroid dehydrogenase was selectively present and very active in the proximal and distal convoluted tubules, particularly of the juxta-medullary glomeruli. This function is thought to be related to the excretion of 3α-ketosteroids. 11β-Hydroxysteroid dehydrogenase was confined to the collecting tubules and its possible involvement in the metabolism of cortisol, aldosterone or androgens in the kidney is noted. 17β-Hydroxysteroid dehydrogenase may be concerned in the excretion of the sex steroids; it occurs throughout the nephron. Δ5-3β-, 16α-, and 16β-hydroxysteroid dehydrogenases were not as active histochemically in the kidney as the 3α-, 3β-, 11β- and 17β-hydroxysteroid dehydrogenases.

CORTISOL UTILIZATION BY SALIVARY GLAND, KIDNEY AND ADRENAL CORTEX

1970 ◽  
Vol 47 (4) ◽  
pp. 511-515 ◽  
Author(s):  
M. M. FERGUSON ◽  
J. B. GLEN ◽  
D. K. MASON
Keyword(s):  
Enzyme Activity ◽  
Sex Differences ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Dehydrogenase Activity ◽  
Adrenal Cortex ◽  
Hydroxysteroid Dehydrogenase ◽  
Histochemical Technique ◽  
Hydroxysteroid Dehydrogenases ◽  
Convoluted Tubules

SUMMARY Cortisol utilization by salivary glands, kidneys and adrenals of various mammals has been compared by using a standard histochemical technique for the demonstration of hydroxysteroid dehydrogenases. 11β-Hydroxysteroid dehydrogenase activity was localized in salivary gland ducts, renal collecting and convoluted tubules and in the adrenal cortex of some species. There was no obvious relationship between the levels of enzyme activity in the salivary glands, kidneys and adrenals. Neither was the presence of 11β-hydroxysteroid dehydrogenase in salivary glands particularly associated with mucous or serous secretion, nor were sex differences in levels of activity evident.

THE INFLUENCE OF HAEMOGLOBIN-S AND G6PD DEFICIENCY ON THE ACTIVITY OF THE 17β-HYDROXYSTEROID DEHYDROGENASE OF INTACT HUMAN ERYTHROCYTES

1974 ◽  
Vol 75 (4) ◽  
pp. 793-800
Author(s):  
A. O. Sogbesan ◽  
O. A. Dada ◽  
B. Kwaku Adadevoh
Keyword(s):  
Enzyme Activity ◽  
Dehydrogenase Activity ◽  
Sex Steroids ◽  
G6pd Deficiency ◽  
Incubation Medium ◽  
Hydroxysteroid Dehydrogenase ◽  
G6pd Activity ◽  
Reverse Transformation ◽  
Oxidative Transformation ◽  
Haemoglobin S

ABSTRACT The 17β-hydroxysteroid dehydrogenase activity in intact erythrocytes of Nigerian patients, in particular with regard to haemoglobin genotypes and G6PD* activity was studied. The G6PD activity of the erythrocyte did not affect the oxidative transformation of testosterone to androstenedione and of oestradiol to oestrone. The reduction (reverse transformation) was inhibited in G6PD-deficient erythrocytes but this inhibition was offset by the addition of 0.025 m glucose to the incubation medium. The per cent oxidation transformation of testosterone was higher in Hb-AA than in Hb-SS erythrocytes. It is suggested that the differences may be a result of either lower enzyme activity in the Hb-SS erythrocytes or of differences in the uptake and possibly binding of sex steroids by intact Hb-SS and Hb-AA erythrocytes.

scholarly journals Human Cytosolic 3α-Hydroxysteroid Dehydrogenases of the Aldo-keto Reductase Superfamily Display Significant 3β-Hydroxysteroid Dehydrogenase Activity

2003 ◽  
Vol 279 (11) ◽  
pp. 10784-10795 ◽  
Author(s):  
Stephan Steckelbroeck ◽  
Yi Jin ◽  
Sridhar Gopishetty ◽  
Busola Oyesanmi ◽  
Trevor M. Penning
Keyword(s):  
Dehydrogenase Activity ◽  
Hydroxysteroid Dehydrogenase ◽  
Hydroxysteroid Dehydrogenases

HYDROXYSTEROID DEHYDROGENASES IN THE HUMAN ADRENAL CORTEX

1966 ◽  
Vol 34 (4) ◽  
pp. 439-NP ◽  
Author(s):  
K. C. CALMAN ◽  
A. H. BAILLIE ◽  
M. M. FERGUSON ◽  
D. McK. HART
Keyword(s):  
Dehydrogenase Activity ◽  
Cushing’S Syndrome ◽  
Adrenal Glands ◽  
Adrenal Adenoma ◽  
Hydroxysteroid Dehydrogenase ◽  
Cushing's Syndrome ◽  
Normal Adult ◽  
Zona Fasciculata ◽  
Adult Human ◽  
Hydroxysteroid Dehydrogenases

SUMMARY The histochemical utilization of 3α-, 3β-, 6β-, 11α-, 11β-, 12α-, 16α-, 16β-, 17α-, 17β-, 20α-, 21- and 24-hydroxysteroids by three normal adult human adrenal glands, two human foetal adrenal glands, three adrenals from patients with Cushing's syndrome and one adrenal adenoma are described. The normal adult human adrenal showed high 16β-hydroxysteroid dehydrogenase activity in the zona glomerulosa. Activity restricted to the outer part of the zona fasciculata was recorded with 3α-, 3β-, 6β-, 11β-, 16α-, 16β-, and 17β-hydroxysteroids. The zona reticularis utilized 3α-, 3β-, 11β-, 16β- and 17β-hydroxysteroids less well than the zona fasciculata. The adrenals of Cushing's syndrome showed activity only for 3β- and 16β-hydroxysteroid dehydrogenases; this activity was noted in all three zones. The activity pattern of the adrenal adenoma resembled that of the normal adult human adrenal except that greater activity for 16α-hydroxysteroid dehydrogenase was noted. The foetal part of the human foetal cortex was extremely active, showing 3α-, 3β-, 6β-, 11β-, 12α-, 16α-, 16β-, 17β-, 20β- and 21-hydroxysteroid dehydrogenase activity. The definitive cortex behaved similarly to the adult gland and possessed 3α-, 3β-, 11β-, 16β- and 17β-hydroxysteroid dehydrogenases; some evidence of zoning of the definitive cortex was seen with the 16β-hydroxysteroid. The relevance of these findings in the light of current knowledge of adrenal zonation is discussed.

3β-HYDROXYSTEROID DEHYDROGENASE IN THE FOETAL MOUSE LEYDIG CELL

1964 ◽  
Vol 31 (1) ◽  
pp. 63-NP ◽  
Author(s):  
A. H. BAILLIE ◽  
K. GRIFFITHS
Keyword(s):  
Dehydrogenase Activity ◽  
Leydig Cell ◽  
Hydroxysteroid Dehydrogenase ◽  
Hydroxysteroid Dehydrogenases ◽  
Weak Activity ◽  
Mouse Leydig Cell

SUMMARY Five male foetal mice were killed daily from the 11th day of gestation until term, the 21st day. Sections of testis from every animal were incubated with three steroid substrates to demonstrate 3β-hydroxysteroid dehydrogenase histochemically. The substrates were (1) 3β-hydroxypregn-5-en-20-one (pregnenolone), (2) 3β,17α-dihydroxypregn-5-en-20-one (17α-hydroxypregnenolone), and (3) 3β-hydroxyandrost-5-en-17-one (DHA). With pregnenolone as substrate 3β-hydroxysteroid dehydrogenase activity was demonstrable from the 11th day, when the testis is first histologically recognizable, until the end of gestation. Using 17α-hydroxypregnenolone, no 3β-hydroxysteroid dehydrogenase activity was present in the foetal testis. With DHA as substrate weak activity was first seen in the testis of the 15-day foetal mouse, and increased steadily thereafter. These findings are thought to support the concept of substrate-specific 3β-hydroxysteroid dehydrogenases.

ACUTE ENZYME HISTOCHEMICAL CHANGES IN THE ZONA GLOMERULOSA OF THE RAT ADRENAL CORTEX

1968 ◽  
Vol 59 (3) ◽  
pp. 508-518
Author(s):  
J. D. Elema ◽  
M. J. Hardonk ◽  
Joh, Koudstaal ◽  
A. Arends
Keyword(s):  
Peritoneal Dialysis ◽  
Succinate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Adrenal Cortex ◽  
Isocitrate Dehydrogenase ◽  
Hydroxysteroid Dehydrogenase ◽  
Zona Glomerulosa ◽  
Acute Changes ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Histochemical Changes

ABSTRACT Acute changes in glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase activity in the zona glomerulosa of the rat adrenal cortex were induced by peritoneal dialysis with 5 % glucose. Although less clear, the activity of 3β-ol-hydroxysteroid dehydrogenase also seemed to increase as well. No changes were seen in the activity of succinate dehydrogenase. Dialysis with 0.9 % NaCl had no effect on any of the enzymes investigated. The possible significance of these observations is discussed.

Sign in / Sign up

Export Citation Format

Share Document