SOME LIGHT- AND ELECTRON-MICROSCOPICAL OBSERVATIONS ON THE PARS TUBERALIS OF THE PITUITARY GLAND OF THE RABBIT

1972 ◽  
Vol 54 (3) ◽  
pp. 505-511 ◽  
Author(s):  
E. CAMERON ◽  
C. L. FOSTER
Keyword(s):  
Interstitial Cell ◽  
Secretory Granules ◽  
The Other ◽  
Neurosecretory Material ◽  
Pars Tuberalis ◽  
Nerve Fibres ◽  
Microscopical Level ◽  
Electron Microscopical Level ◽  
Irregular Nucleus ◽  
Electron Microscopical

SUMMARY The cytological appearance of the pars tuberalis both at the light- and the electron-microscopical levels is described. Two main types of cell, one, which is designated the tuberalis cell, having a rounded contour, pale cytoplasm with a few small secretory granules of about 100 nm in diameter, many polyribosomes and a relatively large ovoid nucleus; and the other, the interstitial cell, with long processes often encircling tuberalis cells, an irregular nucleus and cytoplasm with abundant microfilaments about 10 nm in diameter were seen. Beaded nerve fibres and neurosecretory material were demonstrated in the pars tuberalis with chrome alum—haematoxylin, a finding confirmed at the electron-microscopical level where nerve fibres which appeared to be making contact with tuberalis cells and containing numerous microvesicles of about 50 nm in diameter were observed.

SOME LIGHT- AND ELECTRON-MICROSCOPICAL OBSERVATIONS ON THE PARS INTERMEDIA OF THE PITUITARY GLAND OF THE RABBIT

1971 ◽  
Vol 49 (3) ◽  
pp. 479-485 ◽  
Author(s):  
E. CAMERON ◽  
C. L. FOSTER
Keyword(s):  
Synaptic Vesicles ◽  
The Other ◽  
Neurosecretory Material ◽  
Pars Intermedia ◽  
Neurosecretory Granules ◽  
Intercellular Spaces ◽  
Nerve Fibres ◽  
Aldehyde Fuchsin ◽  
Glandular Cells ◽  
Electron Microscopical

SUMMARY The cytological appearance of the pars intermedia of the rabbit both at the light and the electron microscopical (E.M.) levels, is described. Two types of cell,one stainable byperiodic acid—Schiff's reagent and aldehyde—fuchsin and the other chromophilic, non-granular and intercalated between the former, were observed. In E.M. sections the first type had two kinds of granule in its cytoplasm, a few electron-dense about 250 nm in diameter, mostly confined to the Golgi area, and numerous large 'vesicles' or granules of varying density about 500–600 nm in diameter generally distributed throughout the cytoplasm. The second type of cell when viewed in the electron microscope is characterized by microvilli projecting into intercellular spaces, and long, linked processes ramifying in between the principal cells. Varicose nerve fibres and abundant neurosecretory material were demonstrated in the pars intermedia with chrome alum—haematoxylin and Alcian blue. In E.M. sections these neurosecretory granules were similar to those in the infundibular process. At the point where nerve fibres appeared to be making contact with glandular cells, synaptic vesicles of about 50 nm in diameter were also observed.

Ultrastructural localization of neuropeptides in the rat brain

1978 ◽  
Vol 36 (2) ◽  
pp. 612-613
Author(s):  
F.W. Van Leeuwen
Keyword(s):  
Freeze Substitution ◽  
Ultrastructural Localization ◽  
Serial Sections ◽  
Microscopical Level ◽  
Electron Microscopical Level ◽  
Enzyme Method ◽  
Perfusion Fixation ◽  
Electron Microscopical ◽  
Unlabeled Antibody ◽  
Peroxidase Conjugate

In order to obtain specific and optimal ultrastructural localization of vasopressin and oxytocin in the hypothalamo-neurohypophyseal system of the rat, 2 staining procedures and several tissue treatments were evaluated using neurohypophyseal tissue. It appeared from these studies that post-embedding staining with the unlabeled antibody enzyme method developed by Sternberger allows greater dilution of the first antibody (anti-vasopressin, 1:4800) than the indirect procedure (1:320) using a peroxidase conjugate as second antibody. Immersion fixation with 4% formalin during 24 hours gave better results (general ultrastructure, immunoreactivity) than those obtained by perfusion fixation with 2.5% glutaraldehyde-1% paraformaldehyde or freeze substitution.Since no reliable specificity tests were performed at the electron microscopical level, tests were developed for antibodies against both vasopressin and oxytocin. For anti-vasopressin plasma neural lobes of homozygous Brattleboro rats, that are lacking vasopressin by a genet- ical defect, were used. For antibodies against both hormones serial sections were used that were alternately incubated with the antibodies.

Micromorphometric Determination of Organelles and of Storage Material in Wood Ray Cells - A Useful Method for Detecting Differentiation Within a Tissue

IAWA Journal ◽  
1989 ◽  
Vol 10 (4) ◽  
pp. 395-403 ◽  
Author(s):  
Jörg J. Sauter ◽  
Barbara van Cleve
Keyword(s):  
Individual Cell ◽  
Cell Types ◽  
Storage Material ◽  
Cell Organelles ◽  
Microscopical Level ◽  
Storage Compounds ◽  
Electron Microscopical Level ◽  
Ray Cells ◽  
Electron Microscopical

The size and distribution of individual cell organelles (plastids, mitochondria, oleosomes, protein bodies) and of the three main storage compounds (starch, fat, protein) have been studied micromorphometrically at the electron microscopical level in ray cells of poplar wood during early winter. The three cell types of the rays (contact cells, isolation cells, cells of the contact cell rows) show remarkable differences in size and distribution of organelles and of storage material which manifest an existing physiological specialisation of these cells. The micromorphometric data on storage compounds are compared with the biochemically determined amounts of starch, proteins, fat-bound glycerol, and of various sugars in the wood. At the stage investigated, a prominent protein storage, an extensive starch-sugar transition, but no indications for a starch-fat transition are found. Micromorphometry proved to be a useful tool for the detection of cell-specific differences within a tissue.

Immunocytochemical localization of dopamine in the prefrontal cortex of the rat at the light and electron microscopical level

Neuroscience ◽  
1987 ◽  
Vol 22 (3) ◽  
pp. 849-862 ◽  
Author(s):  
C.G. Van Eden ◽  
E.M.D. Hoorneman ◽  
R.M. Buijs ◽  
M.A.H. Matthijssen ◽  
M. Geffard ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Immunocytochemical Localization ◽  
Microscopical Level ◽  
Electron Microscopical Level ◽  
Electron Microscopical

Acid Phosphatase Activity in the Neutral Red Granules of Mouse Exocrine Pancreas Cells

1964 ◽  
Vol s3-105 (71) ◽  
pp. 343-348
Author(s):  
JENNIFER M. BYRNE
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Exocrine Pancreas ◽  
Neutral Red ◽  
Pancreatic Tissue ◽  
Microscopical Level ◽  
Electron Microscopical Level ◽  
Electron Microscopical

The Gomori test for acid phosphatase was performed on pancreatic tissue from mice injected with neutral red. On an electron microscopical level, acid phosphatase activity was found to be localized in the neutral red granules. It is suggested that the neutral red granules are of lysosomal derivation.

REGENERATION OF HYPOTHALAMIC NERVE FIBRES AFTER HYPOPHYSECTOMY IN THE GOAT

1970 ◽  
Vol 64 (4) ◽  
pp. 696-704 ◽  
Author(s):  
Peter M. Daniel ◽  
Marjorie M. L. Prichard
Keyword(s):  
Median Eminence ◽  
Distal Part ◽  
Neural Tissue ◽  
Neurosecretory Material ◽  
Pars Tuberalis ◽  
Posterior Lobe ◽  
Nerve Fibres ◽  
Traumatic Lesion ◽  
Paraventricular Nuclei ◽  
Regenerating Nerve

ABSTRACT In goats kept for several months after hypophysectomy it was found that the nerve fibres of the hypothalamo-neurohypophysial tract had regenerated. A posterior lobe-like organ had formed in the neural tissue of the median eminence just proximal to the site where the nerve tract had been severed when the pituitary gland was removed. This new, small, ectopic infundibular process was not only well innervated but also highly vascularised and it contained large amounts of neurosecretory material. Some of the regenerating nerve fibres had grown out from the nerve tract into pars tuberalis and the meninges; many of these nerve fibres carried neurosecretory material. In one goat, not hypophysectomized but with a traumatic lesion of the nerve tract in the pituitary stalk, regenerating nerve fibres had also grown down across the scar of the lesion to reinnervate the degenerate distal part of the nerve tract. Within the hypothalamus the loss of nerve cells was consistently greater in the supraoptic than in the paraventricular nuclei.

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