A stereotaxic atlas for diencephalic nuclei of the frog, Rana pipiens.

Masaru WADA; Akihisa URANO; Aubrey GORBMAN

doi:10.1679/aohc1950.43.157

A stereotaxic atlas for diencephalic nuclei of the frog, Rana pipiens.

Archivum histologicum japonicum ◽

10.1679/aohc1950.43.157 ◽

1980 ◽

Vol 43 (2) ◽

pp. 157-173 ◽

Cited By ~ 73

Author(s):

Masaru WADA ◽

Akihisa URANO ◽

Aubrey GORBMAN

Keyword(s):

Rana Pipiens ◽

Stereotaxic Atlas

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Comparative Morphology of Intercellular Bridges Between Developing Germ Cells of the Ovary

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068631 ◽

1970 ◽

Vol 28 ◽

pp. 328-329

Author(s):

J. R. Ruby ◽

R. F. Dyer ◽

R. G. Skalko ◽

R. F. Gasser ◽

E. P. Volpe

Keyword(s):

Germ Cells ◽

Rana Pipiens ◽

Comparative Morphology ◽

Dense Material ◽

Individual Species ◽

Microscope Examination ◽

Electron Dense Material ◽

Intercellular Bridges ◽

Cytoplasmic Side ◽

Intercellular Connections

An electron microscope examination of fetal ovaries has revealed that developing germ cells are connected by intercellular bridges. In this investigation several species have been studied including human, mouse, chicken, and tadpole (Rana pipiens). These studies demonstrate that intercellular connections are similar in morphology regardless of the species.Basically, all bridges are characterized by a band of electron-dense material on the cytoplasmic side of the tri-laminar membrane surrounding the connection (Fig.l). This membrane is continuous with the plasma membrane of the conjoined cells. The dense material, however, never extends beyond the limits of the bridge. Variations in the configuration of intercellular connections were noted in all ovaries studied. However, the bridges in each individual species usually exhibits one structural characteristic seldom found in the others. For example, bridges in the human ovary very often have large blebs projecting from the lateral borders whereas the sides of the connections in the mouse gonad merely demonstrate a slight convexity.

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The application of video-enhanced constrast/differential interference constrast microscopy in conjunction with whole mount electron microscopy to the study of organelle transport

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102419 ◽

1988 ◽

Vol 46 ◽

pp. 66-67

Author(s):

J. H. Hayden

Keyword(s):

Electron Microscopy ◽

Rana Pipiens ◽

Silver Film ◽

Immunofluorescence Microscopy ◽

Organelle Transport ◽

Linear Element ◽

Whole Mount ◽

Carbon Coated ◽

Linear Elements ◽

Dic Microscopy

In a previous study, Allen video-enhanced constrast/differential interference constrast (AVEC-DIC) microscopy was used in conjunction with immunofluorescence microscopy to demonstrate that organelles and vesicle move in either direction along linear elements composed of microtubules. However, this study was limited in that the number of microtubules making up a linear element could not be determined. To overcome this limitation, we have used AVEC-DIC microscopy in conjunction with whole mount electron microscopy.Keratocytes from Rana pipiens were grown on glass coverslips as described elsewhere. Gold London Finder grids were Formvar- and carbon coated, and sterilized by exposure to ultraviolet light. It is important to select a Formvar film that gives a grey reflection when it is floated on water. A silver film is too thick and will detract from the image in the light microscope.

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