Protocol for CUBIC Clearing and Whole Mount Imaging of Mouse Lung Lobes v1

2021 ◽  
Author(s):  
Justinn Barr ◽  
Jamie Verheyden ◽  
Xin Sun
Keyword(s):  
Laboratory Animal ◽  
Computational Analysis ◽  
American Association ◽  
Mouse Lung ◽  
Whole Mount ◽  
Mouse Lung Tissue ◽  
Laboratory Animal Facility ◽  
The University ◽  
Animal Care ◽  
Lung Lobes

This protocol is for Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis (CUBIC) of mouse lung tissue for whole lobe imaging using Zeiss Lightsheet Imaging. All experimental procedures were performed in the American Association for Accreditation of Laboratory Animal Care (AAALAC)-certified laboratory animal facility at the University of California San Diego, following protocols approved by the institutional animal care and use committee (IACUC). The procedures should incorporate all local requirements for standards of animal experimentation.

scholarly journals Laboratory animal facility management

2017 ◽  
Vol 55 (3) ◽  
pp. 268
Author(s):  
N. G. KOSTOMITSOPOULOS (Ν.Γ. ΚΩΣΤΟΜΗΤΣΟΠΟΥΛΟΣ)
Keyword(s):  
Laboratory Animal ◽  
Health And Safety ◽  
Management Program ◽  
Facility Management ◽  
Successful Management ◽  
Laboratory Animal Facility ◽  
Electromechanical Equipment ◽  
Animal Care ◽  
Training Of Personnel ◽  
And Training

The successful management of a laboratory animal facility is based on the design and implementation of a management program, which in most cases covers the minimum legislative requirements and goes further, in order to achieve more in the field of animal welfare. A complete management program should consist of the following main points: a) Monitoring of animal housing, the macro- and microenvironment of the animals, b) veterinary medical care, c) monitoring of electromechanical equipment and the overall construction of the animal facility, and designing of emergency and disaster plans, d) monitoring of the overall program by the Institutional Animal Care and Use Committee responsible to oversee and evaluate the management program of the facility, e) education and training of personnel and f) the implementation of an occupational health and safety program.

THE PRESIDENT'S REPORT (1889)

1978 ◽  
Vol 5 (1) ◽  
pp. 69-71
Author(s):  
John Heins
Keyword(s):  
American Association ◽  
General Meeting ◽  
Annual General ◽  
Annual General Meeting ◽  
University Of Florida ◽  
Public Accountants ◽  
The University

This represents an extract from the handwritten minutes of the Annual General Meeting of the American Association of Public Accountants, Monday, May 27, 1889. The extract is the Report of the President John Heins. This version, in typewritten form came to the attention of researchers at the University of Florida in 1971.

Learning organizations and animal care programs in the absence of appropriate national legislation

2021 ◽  
pp. 002367722110192
Author(s):  
Lazara Martínez-Muñoz
Keyword(s):  
Latin American ◽  
Laboratory Animal ◽  
Management Education ◽  
Education Programs ◽  
Animal Science ◽  
Five Disciplines ◽  
Research Facilities ◽  
Animal Care ◽  
Latin American Countries ◽  
Working Method

The absence, in many nations, of appropriate and corresponding legislation for the protection of experimental animals as well as continual management education programs, significantly affects the inclusion and recognition of experimental results, worldwide. For more than a decade, researchers from Latin American countries have unsuccessfully struggled to get proper legislation. Until today, not many effective results have been seen. After reviewing previous literature and carefully analyzing the available methodologies and practical examples, this paper aims at redesigning the actions and strategies of the members of the research facilities to implement an effective laboratory animal care and use program, and permit the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC) accreditation, independent of national legislative network .This paper also suggests a domestic working method for the teamwork to assume international harmonized legislation, through the application of the Five Disciplines stated by Senge, as methodological process linked with laboratory animal science as scientific background.

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