Freely Moving Visuomotor Learning Assay with Reversal Learning v2

2022 ◽  
Author(s):  
Constantinos Eleftheriou
Keyword(s):  
Reversal Learning ◽  
Visuomotor Learning ◽  
Learning Paradigm ◽  
Last Stage ◽  
Freely Moving

The goal of this protocol is to assess visuomotor learning and motor flexibility in freely-moving mice, using the Visiomode touchscreen platform. It modifies the original protocol's (dx.doi.org/10.17504/protocols.io.bumgnu3w) last stage by replacing forelimb reaching with a reversal learning paradigm

Freely Moving Visuomotor Learning Assay with Reversal Learning v1

2022 ◽  
Author(s):  
Constantinos Eleftheriou
Keyword(s):  
Reversal Learning ◽  
Visual Stimulus ◽  
Visual Stimuli ◽  
Visuomotor Learning ◽  
Water Reward ◽  
Motor Strategy ◽  
Freely Moving

The goal of this protocol is to assess visuomotor learning and motor flexibility in freely-moving mice, using the Visiomode touchscreen platform. Water-restricted mice first learn to associate touching a visual stimulus on the screen with a water reward. They then learn to discriminate between different visual stimuli on the touchscreen by nose-poking, before asked to switch their motor strategy to forelimb reaching.

Freely Moving Visual Discrimination Assay with Visiomode v2

2021 ◽  
Author(s):  
Constantinos Eleftheriou
Keyword(s):  
Citric Acid ◽  
Visual Stimulus ◽  
Visual Discrimination ◽  
Water Deprivation ◽  
Visual Stimuli ◽  
Water Restriction ◽  
Visuomotor Learning ◽  
Water Reward ◽  
Motor Strategy ◽  
Freely Moving

The goal of this protocol is to assess visuomotor learning and motor flexibility in freely-moving mice, using the Visiomode touchscreen platform. Water-restricted mice first learn to associate touching a visual stimulus on the screen with a water reward. They then learn to discriminate between different visual stimuli on the touchscreen by nose-poking, before asked to switch their motor strategy to forelimb reaching. Version 1 of the protocol uses traditional water deprivation and water rewards in the task as a means of motivating mice to perform the task. Version 2 of the protocol uses Citric Acid for water restriction and sucrose as rewards in the task instead of the traditional water deprivation protocol.

Sub-chronic psychotomimetic phencyclidine induces deficits in reversal learning and alterations in parvalbumin-immunoreactive expression in the rat

2007 ◽  
Vol 21 (2) ◽  
pp. 198-205 ◽  
Author(s):  
Z. Abdul-Monim ◽  
J. C. Neill ◽  
G. P. Reynolds
Keyword(s):  
Frontal Cortex ◽  
Reversal Learning ◽  
Cognitive Deficit ◽  
Chronic Administration ◽  
Experimental Period ◽  
Motor Area ◽  
Repeated Treatment ◽  
Acute Administration ◽  
Learning Paradigm ◽  
Immunohistochemical Techniques

Acute administration of the psychotomimetic phencyclidine (PCP) can mimic some features of schizophrenia, while a repeated treatment regimen of PCP may provide a more effective way to model in animals the enduring cognitive dysfunction observed in many schizophrenic patients. The present study aims to investigate behavioural and neuropathological effects of sub-chronic PCP administration. The cognitive deficit induced by sub-chronic PCP was examined using a previously established operant reversal-learning paradigm. Subsequently, the effect of sub-chronic PCP on parvalbumin-immunoreactive (parvalbumin-IR) neurons was assessed using immunohistochemical techniques. Rats were trained to respond for food in an operant reversal-learning paradigm for approximately 6 weeks, followed by sub-chronic administration of PCP (2mg/kg) or vehicle twice daily for 7 days followed 7 days later by behavioural testing. Six weeks post PCP, brains were analysed using immunohistochemical techniques to determine the size and density of parvalbumin-IR in the frontal cortex and hippocampus. Sub-chronic PCP significantly reduced ( p <0.001) percentage correct responding in the reversal phase relative to the initial phase, an effect that persisted throughout the experimental period (4 weeks). The density of parvalbumin-IR neurons was reduced in the hippocampus, with significant reductions in the dentate gyrus and CA2/3 regions ( p <0.001). There were significant changes in the frontal cortex, with a reduction ( p <0.01) in the M1 (motor area 1) region and increases in the M2 (motor area 2) region and cingulate cortex ( p <0.01- p <0.001). These results parallel findings of profound hippocampal and more subtle cortical deficits of parvalbumin-IR neurons in schizophrenia, and provide evidence to suggest that sub-chronic PCP can induce a lasting cognitive deficit, an effect that may be related to the observed neuronal deficits.

Freely Moving Visual Discrimination Assay with Visiomode v2

2021 ◽  
Author(s):  
Constantinos Eleftheriou
Keyword(s):  
Visual Stimulus ◽  
Visual Discrimination ◽  
Visual Stimuli ◽  
Visuomotor Learning ◽  
Water Reward ◽  
Motor Strategy ◽  
Freely Moving

The goal of this protocol is to assess visuomotor learning and motor flexibility in freely-moving mice, using the Visiomode touchscreen platform. Water-restricted mice first learn to associate touching a visual stimulus on the screen with a water reward. They then learn to discriminate between different visual stimuli on the touchscreen by nose-poking, before asked to switch their motor strategy to forelimb reaching.

Sign in / Sign up

Export Citation Format

Share Document