EFFECTS OF ULTRASOUND ON SOMATIC EMBRYOGENESIS AND ARTIFICIAL SEED PRODUCTION IN CUCUMBER (Cucumis sativus L.)

Cucumber (Cucumis sativus L.), a popular vegetable crop of the family Cucurbitaceae, is cultivated in different parts of the world and is of great economic importance. Ultrasound is known as a physical stimulus that can affect living systems. At the right frequency and exposure period, these waves have desired effects. In the present study, 2-week-old plants, were put in an ultrasonic bath with a nominal frequency of 40 kHz, a central frequency of 34/722 kHz and a bandwidth of 320 Hz for 0, 5, 10 and 15 minutes. Hypocotyl pieces were used as explants and cultured in Murashige and Skoog medium supplemented with 0/5 mg/l each of 2,4-dichlorophenoxyacetic acid, 1-Naphthalene acetic acid, kinetin and 6-benzylaminopurine. The use of ultrasonication at a nominal frequency of 40 kHz for 10 minutes showed better results as compared with the samples treated for 0, 5 and 15 minutes, in terms of the shape, maturity of the embryos, and also the germination of artificial seeds and maturing to flowering stage. Since the cucumber of Esfahan is very famous for its aroma and taste and it is somewhat at risk of extinction, we studied the production of artificial seeds of this valuable plant using somatic embryos and apical buds. This research is the first report on investigation of the positive effect of ultrasound on somatic embryogenesis and artificial seed production. our results clearly showed that this method is a practical method to accelerate seed germination and flowering stage.

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Efficacy of drug bathing of sodium nifurstyranate to Vibrio disease of juvenile ayu in the artificial seed production.

Fish Pathology ◽

10.3147/jsfp.23.67 ◽

1988 ◽

Vol 23 (1) ◽

pp. 67-68

Author(s):

Kazuo IWATA ◽

Tsukasa NASU ◽

Takesi TABARU

Keyword(s):

Seed Production ◽

Artificial Seed ◽

Artificial Seed Production

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Artificial seed production of Pangasiid catfish in the Mekong Delta, Vietnam.

10.1079/ac.92519.20203483627 ◽

2020 ◽

Author(s):

Thanh Phuong Nguyen

Keyword(s):

Seed Production ◽

Mekong Delta ◽

Research Programme ◽

Larval Rearing ◽

Growth And Survival ◽

Seed Supply ◽

Artificial Seed ◽

Research Findings ◽

Artificial Seed Production ◽

Pangasius Bocourti

Abstract The two pangasiid catfish, Pangasius bocourti and P. hypophthalmus, are widely cultured in the Mekong Delta, Vietnam. However, seed supply until recently was dependent on fry and fingerlings caught from the Mekong River. In 1994, a research programme was carried out on artificial seed production of these two species in captive conditions. In 1995, about 30,000 fingerlings were first produced in An Giang and Can Tho provinces and a proper technique of induced breeding, progressive human chorionic gonadotropin (hCG) treatment, was determined. This technique, involving two steps of hCG injections, successfully induced ovulation in 66% of 67 treated females of P. bocourti. Application of this technique to other pangasiid catfish species such as P. conchophilus, P. hypophthalmus, P. mekongensis, and P. larnaudii also resulted in breeding success. Research on larval rearing has also been conducted to improve the growth and survival of pangasiid larvae. Extension of research findings of induced breeding and larval rearing has allowed mass seed production of pangasiid catfish in the Mekong Delta.

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In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Journal of Bio-Science ◽

10.3329/jbs.v20i0.17722 ◽

2014 ◽

Vol 20 ◽

pp. 99-108 ◽

Cited By ~ 5

Author(s):

MS Islam ◽

MA Bari

Keyword(s):

Medicinal Plants ◽

Seed Production ◽

In Vitro Regeneration ◽

Basal Medium ◽

Nodal Segments ◽

Ms Medium ◽

Mentha Arvensis ◽

Artificial Seed ◽

Artificial Seed Production

Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion: The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations DOI: http://dx.doi.org/10.3329/jbs.v20i0.17722 J. bio-sci. 20: 99-108, 2012

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