scholarly journals Somatic Embryogenesis of Cucumber (Cucumis sativus L) Using Seed Cuttings Obtained from Pre-Mature Fruit

2003 ◽  
Vol 20 (4) ◽  
pp. 275-281 ◽  
Author(s):  
Ahmed M. HASSANEIN
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Mature Fruit ◽  
Cucumis Sativus L

scholarly journals Temperature Effects on Imbibition and Germination of Cucumber (Cucumis sativus) Seeds

1994 ◽  
Vol 119 (3) ◽  
pp. 464-467 ◽  
Author(s):  
Paul Jennings ◽  
Mikal E. Saltveit
Keyword(s):  
Root Growth ◽  
Cucumis Sativus ◽  
Chilling Tolerance ◽  
Fresh Weight ◽  
Mature Fruit ◽  
Cucumis Sativus L ◽  
Tolerance Level ◽  
Radicle Emergence ◽  
Chilling Temperatures ◽  
Germinating Seeds

Unlike horticulturally mature fruit of `Dasher II' and `Poinsett 76' cucumbers (Cucumis sativus L.), two cultivars that differ significantly in their level of chilling tolerance, imbibing and germinating seeds of these two cultivars responded similarly to chilling temperatures (e.g., increases in fresh weight, time to radicle emergence, and root growth). `Dasher II' and `Poinsett 76' seeds were imbibed and germinated at 10 to 30C, and seeds germinated at 25C for 24 h were chilled at 2.5C for various durations. In comparison, seeds from an aged lot of `Poinsett 76' seed (1989) responded very differently from the 1992 seed lots in all experiments. The chilling tolerance level of germinating `Poinsett 76' seed varied with the seedling age as measured by resumption of root growth. Our results suggest that some factor that confers chilling tolerance is gradually lost during the early stages of germination following imbibition.

scholarly journals COTYLEDON AGE VERSUS IN VlTRO REGENERATION POTENTIAL IN CUCUMBER (CUCUMIS SATIVUS L.)

HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 729a-729
Author(s):  
Nancy J. Zimmerman ◽  
Nancy A. Reichert
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Somatic Embryos ◽  
Fresh Market ◽  
Regeneration Potential ◽  
Cucumis Sativus L ◽  
F1 Hybrid ◽  
Cotyledon Explants ◽  
Media Types ◽  
Cross Country

Seed and seedling cotyledon explants from 14 cultivars were placed on 2 media types to induce organogenesis and somatic embryogenesis. Shoots or somatic embryos were counted to determine effects of cultivar, explant and regeneration type on overall regeneration success. Opposite explant preferences were observed for each regeneration type. In somatic embryogenesis, greater numbers of seedling cotyledons were able to respond, while in organogenesis, seed cotyledons responded in greatest numbers. However, within each cultivar, no explant preferences were observed (except in `Picklebush'). Four cultivars displayed a preference for the somatic embryogenesis regeneration protocol over organogenesis: `Burpee Hybrid II' and `Burpless F1 Hybrid' (fresh market types), and `Cross Country' and `Picklebush' (pickling types). The best individual regeneration rates were obtained with `Cross Country' and `Picklebush' - both for somatic embryogenesis.

scholarly journals EFFECTS OF ULTRASOUND ON SOMATIC EMBRYOGENESIS AND ARTIFICIAL SEED PRODUCTION IN CUCUMBER (Cucumis sativus L.)

2021 ◽  
Vol 18 (2) ◽  
pp. 249-267
Author(s):  
Morvarid Koochani ◽  
Ahmad Majd ◽  
Sedigheh Arbabian ◽  
Faezeh Ghanati ◽  
Sayeh Jafari Marandi
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Seed Production ◽  
Cucumis Sativus L ◽  
Artificial Seed ◽  
Artificial Seed Production

scholarly journals A comparative study on the effects of ultrasound and some growth factors on somatic embryogenesis and artificial seed production in cucumber (Cucumis sativus L.)

2020 ◽  
Vol 48 (4) ◽  
pp. 1915-1928
Author(s):  
Morvarid KOOCHANI ◽  
Ahmad MAJD ◽  
Sedigheh ARBABIAN ◽  
Faezeh GHANATI ◽  
Sayeh JAFARI MARANDI
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Seed Production ◽  
Exposure Period ◽  
Naphthalene Acetic Acid ◽  
Artificial Seeds ◽  
Flowering Stage ◽  
Cucumis Sativus L ◽  
Artificial Seed ◽  
Artificial Seed Production

Cucumber (Cucumis sativus L.), a popular vegetable crop of the family Cucurbitaceae, is cultivated in different parts of the world and is of great economic importance. Ultrasound is known as a physical stimulus that can affect living systems. At the right frequency and exposure period, these waves have desired effects. In the present study, 2-week-old plants, were put in an ultrasonic bath with a nominal frequency of 40 kHz, a central frequency of 34/722 kHz and a bandwidth of 320 Hz for 0, 5, 10 and 15 minutes. Hypocotyl pieces were used as explants and cultured in Murashige and Skoog medium supplemented with 0/5 mg/l each of 2,4-dichlorophenoxyacetic acid, 1-Naphthalene acetic acid, kinetin and 6-benzylaminopurine. The use of ultrasonication at a nominal frequency of 40 kHz for 10 minutes showed better results as compared with the samples treated for 0, 5 and 15 minutes, in terms of the shape, maturity of the embryos, and also the germination of artificial seeds and maturing to flowering stage. Since the cucumber of Esfahan is very famous for its aroma and taste and it is somewhat at risk of extinction, we studied the production of artificial seeds of this valuable plant using somatic embryos and apical buds. This research is the first report on investigation of the positive effect of ultrasound on somatic embryogenesis and artificial seed production. our results clearly showed that this method is a practical method to accelerate seed germination and flowering stage.

scholarly journals Inheritance of Green Mature Seed-stage Fruit Color in Cucumis sativus L.

1992 ◽  
Vol 117 (4) ◽  
pp. 643-645 ◽  
Author(s):  
Gregory C. Peterson ◽  
Leonard M. Pike
Keyword(s):  
Cucumis Sativus ◽  
Fruit Color ◽  
Mature Seed ◽  
Breeding Line ◽  
Mature Fruit ◽  
Cucumis Sativus L ◽  
Major Genes ◽  
Green Fruit ◽  
Commercial Cultivars ◽  
Orange Fruit

Fruit of TAMU breeding line 830397 are green in contrast to the cream or orange fruit of commercial cultivars at the mature-seed stage (MS-S). Inheritance of this trait for green MS-S fruit color in Cucumis sativus was investigated. A new locus, gn, is proposed as well as the elimination of the C locus. MS-S fruit color is controlled by two major genes, R and Gn. Fruit is orange when the genotype is R_ _ and green when the genotype is rrgngn. The cream MS-S fruit color trait is incompletely dominant over green, as the genotype rrGnGn is cream while rrGngn produces mature fruit from cream to intermediate in color between cream-colored and green fruit. Spine color is pleiotropic with or very tightly linked to the R locus, but heavy netting from PI 165509 appears not to be linked with the orange genotype and is polygenic.

scholarly journals GUS Gene expression and plant regeneration via somatic embryogenesis in cucumber (Cucumis sativus L.)

2008 ◽  
Vol 35 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Hyun-A Kim ◽  
Boo-Youn Lee ◽  
Jin-Jung Jeon ◽  
Dong-Woog Choi ◽  
Pil-Son Choi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cucumis Sativus ◽  
Gus Gene ◽  
Cucumis Sativus L ◽  
Gus Gene Expression

scholarly journals Somatic Embryogenesis Using Cucumis sativus (L.) Cotyledons

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 630c-630
Author(s):  
N.M.P. Guedes ◽  
P.H. Jennings
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Embryo Development ◽  
Root Development ◽  
Embryogenic Callus ◽  
Transverse Section ◽  
Cucumis Sativus L ◽  
The Third ◽  
Embryo Formation ◽  
Plantlet Growth

To improve somatic embryogenesis of Cucumis sativus, two types of explants (cotyledons and stem sections) were cultured on Murashige and Skoog (MS) media supplemented with 2,4-D (2.0 mg·L–1) + kinetin (0.5 mg·L–1). After 4 weeks, the embryogenic callus was transferred for 2 weeks to MS + NAA (1.0 mg·L–1) for embryo development. Stem sections failed to develop embryos while cotyledons responded with 14% embryo formation. The embryos were transferred to MS without hormones for 4 weeks to allow for plantlet growth. These embryos developed only shoots. To improve on the successful generation of embryos with root and shoot development, the procedures used above were repeated, but the cotyledons were cut into three sections to be used as explants. Each transverse section of the cotyledon was approximately 2–3 mm wide. All sections produced callus but not all of them were embryogenic. From the first section (cotyledon base), the second (between the first and third section) and the third section (furthest from the cotyledon base), respectively, 58%, 31%, and 5% embryo development occurred. Those embryos from the basal cotyledon sections regenerated 10 plantlets, 5 with shoots and roots and 5 with only shoots. Approaches to enhance somatic embryogenesis, and shoot and root development, will be discussed.

Sign in / Sign up

Export Citation Format

Share Document