Somatic Embryogenesis in Cucumber (Cucumis sativus L.)

1995 ◽  
pp. 151-163 ◽  
Author(s):  
J. A. R. Ladyman
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Cucumis Sativus L

scholarly journals COTYLEDON AGE VERSUS IN VlTRO REGENERATION POTENTIAL IN CUCUMBER (CUCUMIS SATIVUS L.)

HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 729a-729
Author(s):  
Nancy J. Zimmerman ◽  
Nancy A. Reichert
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Somatic Embryos ◽  
Fresh Market ◽  
Regeneration Potential ◽  
Cucumis Sativus L ◽  
F1 Hybrid ◽  
Cotyledon Explants ◽  
Media Types ◽  
Cross Country

Seed and seedling cotyledon explants from 14 cultivars were placed on 2 media types to induce organogenesis and somatic embryogenesis. Shoots or somatic embryos were counted to determine effects of cultivar, explant and regeneration type on overall regeneration success. Opposite explant preferences were observed for each regeneration type. In somatic embryogenesis, greater numbers of seedling cotyledons were able to respond, while in organogenesis, seed cotyledons responded in greatest numbers. However, within each cultivar, no explant preferences were observed (except in `Picklebush'). Four cultivars displayed a preference for the somatic embryogenesis regeneration protocol over organogenesis: `Burpee Hybrid II' and `Burpless F1 Hybrid' (fresh market types), and `Cross Country' and `Picklebush' (pickling types). The best individual regeneration rates were obtained with `Cross Country' and `Picklebush' - both for somatic embryogenesis.

scholarly journals EFFECTS OF ULTRASOUND ON SOMATIC EMBRYOGENESIS AND ARTIFICIAL SEED PRODUCTION IN CUCUMBER (Cucumis sativus L.)

2021 ◽  
Vol 18 (2) ◽  
pp. 249-267
Author(s):  
Morvarid Koochani ◽  
Ahmad Majd ◽  
Sedigheh Arbabian ◽  
Faezeh Ghanati ◽  
Sayeh Jafari Marandi
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Seed Production ◽  
Cucumis Sativus L ◽  
Artificial Seed ◽  
Artificial Seed Production

scholarly journals A comparative study on the effects of ultrasound and some growth factors on somatic embryogenesis and artificial seed production in cucumber (Cucumis sativus L.)

2020 ◽  
Vol 48 (4) ◽  
pp. 1915-1928
Author(s):  
Morvarid KOOCHANI ◽  
Ahmad MAJD ◽  
Sedigheh ARBABIAN ◽  
Faezeh GHANATI ◽  
Sayeh JAFARI MARANDI
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Seed Production ◽  
Exposure Period ◽  
Naphthalene Acetic Acid ◽  
Artificial Seeds ◽  
Flowering Stage ◽  
Cucumis Sativus L ◽  
Artificial Seed ◽  
Artificial Seed Production

Cucumber (Cucumis sativus L.), a popular vegetable crop of the family Cucurbitaceae, is cultivated in different parts of the world and is of great economic importance. Ultrasound is known as a physical stimulus that can affect living systems. At the right frequency and exposure period, these waves have desired effects. In the present study, 2-week-old plants, were put in an ultrasonic bath with a nominal frequency of 40 kHz, a central frequency of 34/722 kHz and a bandwidth of 320 Hz for 0, 5, 10 and 15 minutes. Hypocotyl pieces were used as explants and cultured in Murashige and Skoog medium supplemented with 0/5 mg/l each of 2,4-dichlorophenoxyacetic acid, 1-Naphthalene acetic acid, kinetin and 6-benzylaminopurine. The use of ultrasonication at a nominal frequency of 40 kHz for 10 minutes showed better results as compared with the samples treated for 0, 5 and 15 minutes, in terms of the shape, maturity of the embryos, and also the germination of artificial seeds and maturing to flowering stage. Since the cucumber of Esfahan is very famous for its aroma and taste and it is somewhat at risk of extinction, we studied the production of artificial seeds of this valuable plant using somatic embryos and apical buds. This research is the first report on investigation of the positive effect of ultrasound on somatic embryogenesis and artificial seed production. our results clearly showed that this method is a practical method to accelerate seed germination and flowering stage.

scholarly journals GUS Gene expression and plant regeneration via somatic embryogenesis in cucumber (Cucumis sativus L.)

2008 ◽  
Vol 35 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Hyun-A Kim ◽  
Boo-Youn Lee ◽  
Jin-Jung Jeon ◽  
Dong-Woog Choi ◽  
Pil-Son Choi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Cucumis Sativus ◽  
Gus Gene ◽  
Cucumis Sativus L ◽  
Gus Gene Expression

scholarly journals Somatic Embryogenesis Using Cucumis sativus (L.) Cotyledons

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 630c-630
Author(s):  
N.M.P. Guedes ◽  
P.H. Jennings
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Embryo Development ◽  
Root Development ◽  
Embryogenic Callus ◽  
Transverse Section ◽  
Cucumis Sativus L ◽  
The Third ◽  
Embryo Formation ◽  
Plantlet Growth

To improve somatic embryogenesis of Cucumis sativus, two types of explants (cotyledons and stem sections) were cultured on Murashige and Skoog (MS) media supplemented with 2,4-D (2.0 mg·L–1) + kinetin (0.5 mg·L–1). After 4 weeks, the embryogenic callus was transferred for 2 weeks to MS + NAA (1.0 mg·L–1) for embryo development. Stem sections failed to develop embryos while cotyledons responded with 14% embryo formation. The embryos were transferred to MS without hormones for 4 weeks to allow for plantlet growth. These embryos developed only shoots. To improve on the successful generation of embryos with root and shoot development, the procedures used above were repeated, but the cotyledons were cut into three sections to be used as explants. Each transverse section of the cotyledon was approximately 2–3 mm wide. All sections produced callus but not all of them were embryogenic. From the first section (cotyledon base), the second (between the first and third section) and the third section (furthest from the cotyledon base), respectively, 58%, 31%, and 5% embryo development occurred. Those embryos from the basal cotyledon sections regenerated 10 plantlets, 5 with shoots and roots and 5 with only shoots. Approaches to enhance somatic embryogenesis, and shoot and root development, will be discussed.

scholarly journals In vitro culture of Cucumis sativus L. VI. Histological analysis of leaf explants cultured on media with 2, 4-D or 2, 4, 5-T

2014 ◽  
Vol 56 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Anna Nadolska-Orczyk ◽  
Stefan Malepszy
Keyword(s):  
Somatic Embryogenesis ◽  
Cucumis Sativus ◽  
Histological Analysis ◽  
Leaf Explants ◽  
Vascular Bundles ◽  
Meristematic Tissue ◽  
Cucumis Sativus L ◽  
Meristematic Cells ◽  
Cellular Aggregates

The developmental sequence of callus initiation and somatic embryogenesis in leaf explants of <em>Cucumis sativus</em> cv. Borszczagowski was analysed and compared on media containing two different auxin phenoxy-derivatives (2,4-D and 2,4,5-T) and cytokinin (BAP or 2iP). During the first 20 days of culture on media with 2,4,5-T proliferation of parenchymatic tissue occurred mainly and only small meristematic centers were observed. There was an intensive detachment of parenchymatic cells and dissociation of their cell walls near vessels and in the lower part of the explant adjacent to the medium. These cells were strongly plasmolysed. On the 2,4-D containing medium mostly meristematic tissue developed, proliferating around vascular bundles and forming meristematic centers or promeristem-like structures. After 35-50 days of culture, secondary callus was formed by separation of meristematic cells from the meristem surface in explants cultured on the 2,4-D containing medium. On medium supplemented with 2, 4, 5-T the detachment of parenchymatic and meristematic cells occurred, along with formation of a gel-like substance. The gel-like callus contained multi-cellular aggregates, proembryoids and embryoids. This type of callus tissue was initiated more intensively on medium with 2, 4, 5-T, but the frequency of somatic embryogenesis was much lower. The periferial cells of aggregates, proembryoids and embryoids showed the tendency to separate from the surface of the tissue. Many embryoids formed adventitious embryos.

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