scholarly journals Cloning and partial characterization of a gene in Bombyx mori homologous to a human adiponectin receptor.

2008 ◽  
Vol 55 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Minfeng Zhu ◽  
Keping Chen ◽  
Yong Wang ◽  
Zhongjian Guo ◽  
Huijuan Yin ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Membrane Protein ◽  
Bombyx Mori ◽  
Fat Body ◽  
Receptor Gene ◽  
Amino Acid Sequences ◽  
Malpighian Tubules ◽  
Adiponectin Receptor ◽  
Bombyx Mori Nucleopolyhedrovirus

In this study, we report the cloning and characteristics of an adiponectin-like receptor gene from Bombyx mori (BmAdipoR) with highly conserved deduced amino-acid sequences and similar structure to the human adiponectin receptor (AdipoR). Structural analysis of the translated cDNA suggested it encoded a membrane protein with seven transmembrane domains. BmAdipoR was found to be expressed in multiple tissues and highly expressed in Malpighian tubules, fat body and testis. BmNPV (Bombyx mori nucleopolyhedrovirus) bacmid system combined with confocal microscopy revealed that BmAdipoR was targeted to the cell membrane. We also found that infection with BmNPV did not have an effect on BmAdipoR mRNA quantity in the midgut of susceptible Bombyx mori strain (306) at 48 h, but BmAdipoR mRNA quantity increased significantly at 72 h. We concluded that BmAdipoR gene was a membrane protein ubiquitously expressed in Bombyx mori tissues and that its expression was altered by treating with BmNPV.

Characterization of the open reading frame 7a from Bombyx mori nucleopolyhedrovirus

2012 ◽  
Vol 40 (2) ◽  
pp. 865-873
Author(s):  
Yong Liu ◽  
Feng Yu ◽  
Huiling Wu ◽  
Qing Cao ◽  
Yu Wu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Open Reading Frame ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Reading Frame

scholarly journals Characterization of Bombyx mori nucleopolyhedrovirus with a knockout of Bm17

2012 ◽  
Vol 64 (6) ◽  
pp. 711-718 ◽  
Author(s):  
Hongxing Shen ◽  
Yang Zhou ◽  
Wen Zhang ◽  
Bin Nin ◽  
Hua Wang ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Bombyx Mori Nucleopolyhedrovirus

scholarly journals Characterization of Bombyx mori Nucleopolyhedrovirus orf68 Gene That Encodes a Novel Structural Protein of Budded Virus

Virology ◽  
2002 ◽  
Vol 297 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Masashi Iwanaga ◽  
Masaaki Kurihara ◽  
Masahiko Kobayashi ◽  
WonKyung Kang
Keyword(s):  
Bombyx Mori ◽  
Structural Protein ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Budded Virus

Characterization of an early gene orf122 from Bombyx mori nucleopolyhedrovirus

2008 ◽  
Vol 36 (3) ◽  
pp. 543-548 ◽  
Author(s):  
Jun-Qing Ge ◽  
Jin-Fang Zhao ◽  
Ya-Ming Shao ◽  
Cai-Hong Tian ◽  
Chuan-Xi Zhang
Keyword(s):  
Bombyx Mori ◽  
Early Gene ◽  
Bombyx Mori Nucleopolyhedrovirus

scholarly journals Characterization of a Late Gene, ORF60 from Bombyx mori Nucleopolyhedrovirus

BMB Reports ◽  
2006 ◽  
Vol 39 (6) ◽  
pp. 737-742 ◽  
Author(s):  
Meng-Fang Du ◽  
Xin-Ming Yin ◽  
Zhong-Jian Guo ◽  
Liang-Jun Zhu
Keyword(s):  
Bombyx Mori ◽  
Late Gene ◽  
Bombyx Mori Nucleopolyhedrovirus

Molecular Characterization of Primary Juvenile Hormone Responders Methoprene-Tolerant (Met) and Krüppel Homolog 1 (Kr-h1) in Grapholita molesta (Lepidoptera: Tortricidae) with Clarification of Their Roles in Metamorphosis and Reproduction

2019 ◽  
Vol 112 (5) ◽  
pp. 2369-2380 ◽  
Author(s):  
Jing Zhang ◽  
Xiaoxia Liu ◽  
Yichen Liu ◽  
Yueqing An ◽  
Haibo Fang ◽  
...  
Keyword(s):  
Fat Body ◽  
Larval Mortality ◽  
Adult Emergence ◽  
Amino Acid Sequences ◽  
Grapholita Molesta ◽  
Preoviposition Period ◽  
Krüppel Homolog 1 ◽  
Lepidoptera Noctuidae ◽  
Dsrna Injection

AbstractMethoprene-tolerant (Met) is a putative JH intracellular receptor that transduces JH signal by activation of the inducible Krüppel homolog 1 (Kr-h1). We analyzed the gene sequences of Met and Kr-h1 and their patterns of expression in Grapholita molesta (Busck) immature and adult stages in order to better understand the roles of these primary JH responders in regulating the metamorphosis and reproduction of this global pest of fruit crops. The deduced amino acid sequences of both GmMet and GmKr-h1 were highly homologous to those of other Lepidoptera, especially the cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae). Peak expression of GmMet occurred during the last 3 to 5 d of the final instar, followed by that of GmKr-h1, in the last 3 d of final instar. Similar patterns of GmMet and GmKr-h1 expression were detected across various tissue types in the fifth-instar larvae, with the highest expression observed in the head, followed by the epidermis, and the fat body. When expression of GmMet and GmKr-h1 was knocked down via dsRNA injection in the fifth instar, the results were increased larval mortality, abnormal pupation, delayed pupal duration, reduced adult emergence, extended preoviposition period, and reduced fecundity. We infer that both GmMet and GmKr-h1 participated in regulation of metamorphosis and reproduction in G. molesta, the former acting upstream of the latter, and could present biorational targets for novel pest control compounds.

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