Purification and characterization of a heteromultimeric glycoprotein from Artocarpus heterophyllus latex with an inhibitory effect on human blood coagulation.

Plant latex has many health benefits and has been used in folk medicine. In this study, the biological effect of Artocarpus heterophyllus (jackfruit) latex on human blood coagulation was investigated. By a combination of heat precipitation and ion-exchange chromatography, a heat stable heteromultimeric glycoprotein (HSGPL1) was purified from jackfruit milky latex. The apparent molecular masses of the monomeric proteins on SDS/PAGE were 33, 31 and 29 kDa. The isoelectric points (pIs) of the monomers were 6.63, 6.63 and 6.93, respectively. Glycosylation and deglycosylation tests confirmed that each subunit of HSGPL1 formed the native multimer by sugar-based interaction. Moreover, the multimer of HSGPL1 also resisted 2-mercaptoethanol action. Peptide mass fingerprint analysis indicated that HSGPL1 was a complex protein related to Hsps/chaperones. HSGPL1 has an effect on intrinsic pathways of the human blood coagulation system by significantly prolonging the activated partial thrombin time (APTT). In contrast, it has no effect on the human extrinsic blood coagulation system using the prothrombin time (PT) test. The prolonged APTT resulted from the serine protease inhibitor property of HSGPL1, since it reduced activity of human blood coagulation factors XI(a) and α-XII(a).

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Changes in the blood coagulation system that determine postoperative complications in patients with non-tumor mechanical jaundice

Grekov s Bulletin of Surgery ◽

10.24884/0042-4625-2021-180-2-12-20 ◽

2021 ◽

Vol 180 (2) ◽

pp. 12-20

Author(s):

V. E. Fedorov ◽

B. S. Kharitonov ◽

A. D. Aslanov ◽

O. E. Logvina ◽

M. S. Narizhnaya

Keyword(s):

Postoperative Complications ◽

Blood Coagulation ◽

Blood Platelets ◽

Coagulation Factors ◽

Coagulation System ◽

Thrombin Time ◽

Blood Coagulation Factors ◽

Blood Coagulation System ◽

Normal Limits ◽

Prothrombin Index

The OBJECTIVE was to study the features of changes in the blood coagulation system that contribute to the development of postoperative complications in patients depending on the stage of non-tumor mechanical jaundice at admission.METHODS AND MATERIALS. A total of 537 patients with mechanical jaundice were examined and changes in the blood coagulation system were analyzed. Vascular-platelet hemostasis was characterized by the following tests: capillary resistance, the number of desquamated endothelial cells, the number of blood platelets. Plasma hemostasis was analyzed using activated partial thromboplastin time, plasma soluble fibrin level, thrombin time, prothrombin ratio, prothrombin index, and fibrinogen blood level. Then, XIIa-dependent fibrinolysis in the blood and the level of the fibrin D-dimer in the blood plasma were determined.RESULTS. It was found that in the first stage of mechanical jaundice, with cholestasis, there were no changes in blood coagulation system that go beyond the normal limits. In the second stage, during cytolysis of hepatocytes, hyperbilirubinemia and hypertransaminasemia contribute to the activation of platelet first, and then plasma hemostasis. In the third stage (cholangitis), the death of endotheliocytes increases and there is a deficiency of blood coagulation factors due to their consumption and increased fibrinolysis.CONCLUSION. In the stage of cholestasis in patients with non-tumors mechanical jaundice, the parameters of the coagulation system remain within the reference values. In the stage of cytolysis, as endotheliotoxicosis increases, platelet and plasma hemostasis begins to activate, which can lead to thrombosis and thromboembolism in vital organs. In the stage of cholangitis, further activation of plasma hemostasis causes hemorrhagic syndrome. The occurrence of the described disorders in blood coagulation system with the progression of MJ dictates the need to monitor the changes in the blood coagulation system and their correction for the prevention of intra-and postoperative complications.

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Effect of a fibrinogenase from Vipera lebetina venom on human blood coagulation factors and platelets

Toxicon ◽

10.1016/0041-0101(93)90061-m ◽

1993 ◽

Vol 31 (5) ◽

pp. 520 ◽

Cited By ~ 1

Keyword(s):

Blood Coagulation ◽

Human Blood ◽

Coagulation Factors ◽

Blood Coagulation Factors ◽

Vipera Lebetina ◽

Human Blood Coagulation

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Mammalian Expression Systems for Human Blood Coagulation Factors

Japanese Journal of Thrombosis and Hemostasis ◽

10.2491/jjsth.2.546 ◽

1991 ◽

Vol 2 (6) ◽

pp. 546-549

Author(s):

Tadashi MATSUSHITA ◽

Nobuhiko EMI

Keyword(s):

Blood Coagulation ◽

Human Blood ◽

Coagulation Factors ◽

Expression Systems ◽

Blood Coagulation Factors ◽

Mammalian Expression ◽

Human Blood Coagulation

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The blood coagulation system of rats under the influence of laser radiation in different time parameters

Biolohichni systemy ◽

10.31861/biosystems2019.01.019 ◽

2019 ◽

Vol 11 (1) ◽

pp. 19-26

Author(s):

Oksana Ketsa ◽

Mykhailo Marchenko ◽

Nadiia Bodnarjuk

Keyword(s):

Prothrombin Time ◽

Laser Irradiation ◽

Blood Coagulation ◽

Laser Diode ◽

Coagulation System ◽

Simultaneous Increase ◽

Thrombin Time ◽

Blood Coagulation System ◽

Group I ◽

Time Parameters

The influence of different terms of laser irradiation on vascular-platelet and coagulation units of blood coagulation system is investigated. The rats were irradiated with a laser diode in the red spectrum (650 nm) with a power of 50 mW. Rats were divided into two groups: group I - rats, which were irradiated daily for 2 minutes; group II - rats, which were irradiated daily for 4 minutes. Euthanasia of animals was performed on the 7th and 14th days after the onset of irradiation. For the study of the blood coagulation system of rats used biochemical coagulogram with the analysis of the following indicators - the content of fibrinogen, thrombin and prothrombin time, activated partial thromboplastin time (APTT), platelet count. The content of lymphocytes and erythrocyte sedimentation rate were determined also. In the work used blood plasma with sodium citrate. It has been found that daily four-minute laser irradiation in the abdominal area for two weeks leads to a decrease in the concentration of factor I blood clotting (fibrinogen) and an increase in thrombin time. At the same time, prolongation of prothrombin time and APTT was detected, indicating a deficiency of other factors of blood coagulation - II, V, VII-XII, and the presence of anticoagulant status, which may be associated with inhibition of the internal pathway of clotting. Thrombocytopenia is probably caused by the increased destruction of platelets or their insufficient formation as a result of dysfunction of hematopoietic organs. The two-minute effect of laser irradiation does not affect the clinical and biochemical parameters of the blood coagulation system of rats after a weekly daily action of the laser diode. Two weeks irradiation is accompanied by processes of hypocoagulation, as evidenced by a decrease in fibrinogen levels, platelet counts, and a simultaneous increase in APTT, thrombin and prothrombin time.

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