scholarly journals Modeling the differential phenotypes of spinal muscular atrophy with high-yield generation of motor neurons from human induced pluripotent stem cells

Oncotarget ◽  
2017 ◽  
Vol 8 (26) ◽  
pp. 42030-42042 ◽  
Author(s):  
Xiang Lin ◽  
Jin-Jing Li ◽  
Wen-Jing Qian ◽  
Qi-Jie Zhang ◽  
Zhong-Feng Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spinal Muscular Atrophy ◽  
Induced Pluripotent Stem Cells ◽  
Motor Neurons ◽  
Pluripotent Stem Cells ◽  
Muscular Atrophy ◽  
High Yield ◽  
Induced Pluripotent

scholarly journals Induced pluripotent stem cells from a spinal muscular atrophy patient

Nature ◽  
2008 ◽  
Vol 457 (7227) ◽  
pp. 277-280 ◽  
Author(s):  
Allison D. Ebert ◽  
Junying Yu ◽  
Ferrill F. Rose ◽  
Virginia B. Mattis ◽  
Christian L. Lorson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spinal Muscular Atrophy ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Muscular Atrophy ◽  
Spinal Muscular Atrophy Patient ◽  
Induced Pluripotent

Gene Corrected Spinal Muscular Atrophy-Induced Pluripotent Stem Cells and Motoneuron as a Model and Cell Source for Transplantation (P03.176)

Neurology ◽  
2012 ◽  
Vol 78 (Meeting Abstracts 1) ◽  
pp. P03.176-P03.176
Author(s):  
S. Corti ◽  
M. Nizzardo ◽  
C. Simone ◽  
M. Falcone ◽  
M. Nardini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spinal Muscular Atrophy ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Muscular Atrophy ◽  
Cell Source ◽  
Induced Pluripotent

Gene Corrected Spinal Muscular Atrophy-Induced Pluripotent Stem Cells and Motoneuron as a Model and Cell Source for Transplantation (IN8-2.002)

Neurology ◽  
2012 ◽  
Vol 78 (Meeting Abstracts 1) ◽  
pp. IN8-2.002-IN8-2.002
Author(s):  
S. Corti ◽  
M. Nizzardo ◽  
C. Simone ◽  
M. Falcone ◽  
M. Nardini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spinal Muscular Atrophy ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Muscular Atrophy ◽  
Cell Source ◽  
Induced Pluripotent

scholarly journals Cell freezing protocol optimized for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

2016 ◽  
Author(s):  
Pamela Milani ◽  
Renan Escalante-Chong ◽  
Brandon C. Shelley ◽  
Natasha L. Patel-Murray ◽  
Xiaofeng Xin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Motor Neurons ◽  
Pluripotent Stem Cells ◽  
Muscular Atrophy ◽  
Cell Type ◽  
High Quality ◽  
Induced Pluripotent ◽  
Accessible Chromatin ◽  
Relevant Cell Type

In recent years, the assay for transposase-accessible chromatin using sequencing (ATAC-Seq) has become a fundamental tool of epigenomic research. However, it has proven difficult to perform this technique on frozen samples because freezing cells before extracting nuclei impairs nuclear integrity and alters chromatin structure. We describe a protocol for freezing cells that is compatible with ATAC-Seq, producing results that compare well with those generated from fresh cells. We found that while flash-frozen samples are not suitable for ATAC-Seq, the assay is successful with slow-cooled cryopreserved samples. Using this method, we were able to isolate high quality, intact nuclei, and we verified that epigenetic results from fresh and cryopreserved samples agree quantitatively. We developed our protocol on a disease-relevant cell type, namely motor neurons differentiated from induced pluripotent stem cells from a patient affected by spinal muscular atrophy.

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