Genetic Diversity Analysis in White Yam (Dioscorea rotundata Poir.) using Random Amplified Polymorphic DNA (RAPD) Markers

2019 ◽  
Vol 7 (5) ◽  
pp. 36-42 ◽  
Author(s):  
Parvathy Harikuma ◽  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Diversity Analysis ◽  
Dioscorea Rotundata ◽  
Genetic Diversity Analysis ◽  
White Yam

scholarly journals OPTIMASI SUHU ANNEALING UNTUK EMPAT PRIMER RAPD PADA KACANG HIJAU (Vigna radiata L.)

2019 ◽  
Vol 34 (1) ◽  
pp. 41-46
Author(s):  
Herman Herman ◽  
Martupa Nainggolan ◽  
Dewi Indriyani Roslim
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Annealing Temperature ◽  
Random Amplified Polymorphic Dna ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Temperature Optimization ◽  
Total Dna

Determination of annealing temperature of the primer is the first step for genetic diversity analysis using molecular markers such as RAPD (Random Amplified Polymorphic DNA). This study aims to determine annealing temperature (Ta) of RAPD primers on Kampar Mungbean. Methods included total DNA extraction, electrophoresis, and annealing temperature optimization of four RAPD markers namely OPD-20, OPI-06, OPI-13, dan OPX-13. Optimization was conducted by reducing the Tm value (Time melting) of each primer with 3 (Tm-3) and 5 (Tm-5). The results showed that the optimization using OPD-20 and OPX-13 produced bands at Tm-3 and Tm-5. Meanwhile, optimization using OPI-06 and OPI-13 resulted in bands at Tm-3. The next step was to choose the exact Ta based on the clear and bright band. In conclusion, exact Ta for OPD-20, OPI-06, OPI-13, and OPX-13 were 36,1°C, 38,1°C, 35,4°C, and 32,5°C respectively.

Genetic Diversity Analysis of Starchy Curcuma Species Using RAPD Markers

2008 ◽  
Vol 17 (2) ◽  
pp. 173-176 ◽  
Author(s):  
G. R. Angel ◽  
T. Makeshkumar ◽  
C. Mohan ◽  
B. Vimala ◽  
B. Nambisan
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis

scholarly journals Genetic Diversity Analysis of Lates calcarifer (Bloch 1790) in Captive and Wild Populations Using RAPD Markers

2012 ◽  
Vol 4 (3) ◽  
pp. 33-37 ◽  
Author(s):  
Muthusamy RAJASEKAR ◽  
Muthusamy THANGARAJ ◽  
Thathiredypalli R. BARATHKUMAR ◽  
Jayachandran SUBBURAJ ◽  
Kaliyan MUTHAZHAGAN
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Diversity Analysis ◽  
Wild Populations ◽  
Lates Calcarifer ◽  
Genetic Diversity Analysis

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