diversity analysis
Recently Published Documents


TOTAL DOCUMENTS

1797
(FIVE YEARS 541)

H-INDEX

47
(FIVE YEARS 6)

2022 ◽  
Vol 295 ◽  
pp. 110852
Author(s):  
Shashi Bhushan Choudhary ◽  
Suresh Chandra Gurjar ◽  
Binay Kumar Singh ◽  
Devendra Kumar Singh ◽  
Hariom Kumar Sharma ◽  
...  

2022 ◽  
Vol 8 ◽  
Author(s):  
Ziyin Huang ◽  
Xiaofei Mei ◽  
Yufeng Jiang ◽  
Tan Chen ◽  
Yafeng Zhou

Introduction: Heart failure with preserved ejection fraction (HFpEF) is associated with disrupted intestinal epithelial function, resulting from intestinal congestion. Intestinal congestion changes the morphology and permeability of the intestinal wall, and it becomes easy for the gut microbiota to change and transfer. Intervention on gut microbiota may become a new target for HFpEF treatment. However, the characteristics of gut microbiota in patients with HFpEF remain unknown. This preliminary report aims to detect the structure of gut microbiota in HFpEF patients so as to explore their characteristic changes, thereby providing a theoretical basis for future research.Methods: This research recruited 30 patients diagnosed with HFpEF and 30 healthy individuals. Stool specimens of research subjects were collected separately, and the microarray analyses of gut microbiota were conducted by Illumina high-throughput DNA sequencing. The differences in gut microbiota composition, alpha diversity, and beta diversity between the two groups were finally obtained.Results: The composition of gut microbiota was significantly different between the two groups. At the phylum classification level, the abundance of Synergistetes tended to be higher in the HFpEF group (P = 0.012). At genus classification level, the abundance of Butyricicoccus (P < 0.001), Sutterella (P = 0.004), Lachnospira (P = 0.003), and Ruminiclostridium (P = 0.009) in the HFpEF group were lower, while the abundance of Enterococcus (P < 0.001) and Lactobacillus (P = 0.005) were higher. According to the Chao index of alpha diversity analysis, HFpEF patients showed a nominally significant lower species richness when compared with controls (P = 0.046). However, there was no statistical difference in the Shannon index (P = 0.159) and Simpson index (P = 0.495), indicating that there was no difference in species diversity between the two groups. Beta diversity analysis revealed a highly significant separation of HFpEF patients and controls.Conclusions: An imbalance in the gut microbiota of HFpEF patients was observed. Patients with HFpEF have an increased abundance of microbiota associated with inflammation and a decreased abundance of microbiota associated with anti-inflammatory effects in the gut environment. In line with that, the species richness of gut microbiota in HFpEF patients tended to be lower.


2022 ◽  
Vol 13 (3) ◽  
Author(s):  
Parashuram Dattu Patroti ◽  
Ragimasalawada Madhusudhana ◽  
Sujay Rakshit ◽  
Nitesh Shirur Devaraja ◽  
Kuldeep Kumar Sharma ◽  
...  

2022 ◽  
Author(s):  
Fernando Mejia ◽  
Francisco Avilés Jiménez ◽  
Alfonso Méndez Tenorio

Microbial diversity is the most abundant form of life. Next Generation Sequencing technologies provide the capacity to study complex bacterial communities, in which the depth and the bioinformatic tools can influence the results. In this work we explored two different protocols for bacterial classification and abundance evaluation, using 10 bacterial genomes in a simulated sample at different sequencing. Protocol A consisted of metagenome assembly with Megahit and Ray Meta and taxonomic classification with Kraken2 and Centrifuge. In protocol B only taxonomicclassification. In both protocols, rarefaction, relative abundance and beta diversity were analyzed. In the protocol A, Megahit had a mean contig length of 1,128 and Ray Meta de 8,893 nucleotides. The number of species correctly classified in all depth assays were 6 out of 10 for protocol A, and 9 out of 10 using protocol B. The rarefaction analysis showed an overestimation of the number of species in almost all assays regardless of the protocol, and the beta diversity analysis results indicated significant differences in all comparisons. Protocol A was more efficient for diversity analysis, while protocol B estimated a more precise relative abundance. Our results do not allow us to suggest an optimal sequencing depth at specie level.


2022 ◽  
pp. 02-07
Author(s):  
Ruchismita Mishra ◽  
Roshan Parihar ◽  
AP Agrawal ◽  
DJ Sharma ◽  
NK Chaure ◽  
...  

2022 ◽  
Vol 951 (1) ◽  
pp. 012063
Author(s):  
D Maretta ◽  
Sobir ◽  
I Helianti ◽  
Purwono ◽  
E Santosa

Abstract Fresh taro and its processed products have been produced in Bogor for decades. It has become a popular commodity from this city and evoked an image of Bogor. The essential component of this issue is the sustainability of taro farming, and farmers are the major preserver who has primary decision-making in agricultural management. It assumes there were unusual agricultural practices that promote the long-term viability of taro cultivation. Farmers’ consideration of taro cultivation and their on-farm activities were observed, and the impacted-on sustainability was studied. We surveyed 30 taro farmers in 8 districts Bogor with a rapid rural appraisal and field observation to validate the obtained data and plant diversity analysis. The result showed that taro has provided more income for farmers, and they prefer an intercropping approach in taro farming. Seed availability was the main consideration of farmers in cultivating this plant consistently for a long time. The seed rotation by seed sharing between farmer’s communities was the mechanism of seed exchange and spread of taro cultivars. There was also found the diversity of taro that divided into three groups similarities and simply to distinguish by the plant stature, color of petioles and corms cross-section.


Sign in / Sign up

Export Citation Format

Share Document