Detection of a New Variant in Outbreak of Bovine Ephemeral Fever in Turkey, 2020

Background: In this study, partial nucleotide sequence analysis of the G gene was performed for the molecular characterization of the virus that caused the bovine ephemeral fever virus (BEFV) epidemic in Turkey in 2020. Phylogenetic analysis of these nucleotide sequences was performed with the virus nucleotide sequences of the epidemics seen in 2008 and 2012. These sequences were announced in GenBank. Phylogenetic analysis of these nucleotide sequences was performed with the virus nucleotide sequences of the epidemics seen in 2008 and 2012. Methods: The study was conducted in dairy cattle holdings located in Diyarbakır Sur, Çınar and Dicle regions in South-eastern Turkey in August-November 2020. The number of animals in the holdings consisted of 750 (n=750), 150 (n=150) and 200 (n=200) cattle, respectively. Result: Severe respiratory symptoms and high mortality in the affected animals were notable symptoms. As a result of the phylogenetic analysis, it was determined that the virus that caused the epidemic in Turkey in 2020 was formed by a new variant in the Turkey-2 group, which was similar to the Indian isolates, unlike the Turkey-1 group, which was close to the Middle East variants in 2008 and 2012 isolates.

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Isolation and genetic characterization of bovine ephemeral fever virus from epidemic-2020 in Turkey

Tropical Animal Health and Production ◽

10.1007/s11250-021-02715-1 ◽

2021 ◽

Vol 53 (2) ◽

Author(s):

Ilke Karayel-Hacioglu ◽

Selda Duran Yelken ◽

Yaser Vezir ◽

Nilay Unal ◽

Feray Alkan

Keyword(s):

Genetic Characterization ◽

Fever Virus ◽

Bovine Ephemeral Fever Virus ◽

Bovine Ephemeral Fever

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Genetic characterization of bovine ephemeral fever virus in southern China, 2013–2017

Virus Genes ◽

10.1007/s11262-020-01740-w ◽

2020 ◽

Vol 56 (3) ◽

pp. 390-395 ◽

Cited By ~ 1

Author(s):

Jia Kun ◽

Jia Rongrong ◽

Wang Xiangbin ◽

Zhao Yan ◽

Dou Yiping ◽

...

Keyword(s):

Genetic Characterization ◽

Southern China ◽

Fever Virus ◽

Bovine Ephemeral Fever Virus ◽

Bovine Ephemeral Fever

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The isolation and preliminary characterization of a rhabdovirus in Australia related to bovine ephemeral fever virus

Veterinary Microbiology ◽

10.1016/0378-1135(83)90075-5 ◽

1983 ◽

Vol 8 (3) ◽

pp. 221-235 ◽

Cited By ~ 37

Author(s):

D.H. Cybinski ◽

H. Zakrzewski

Keyword(s):

Fever Virus ◽

Bovine Ephemeral Fever Virus ◽

Preliminary Characterization ◽

Bovine Ephemeral Fever

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Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus

Archives of Virology ◽

10.1007/s00705-016-3078-2 ◽

2016 ◽

Vol 162 (2) ◽

pp. 359-367 ◽

Cited By ~ 9

Author(s):

Minmin Zhang ◽

Jinying Ge ◽

Zhiyuan Wen ◽

Weiye Chen ◽

Xijun Wang ◽

...

Keyword(s):

Newcastle Disease Virus ◽

Disease Virus ◽

Newcastle Disease ◽

Fever Virus ◽

Bovine Ephemeral Fever Virus ◽

Bovine Ephemeral Fever ◽

Recombinant Newcastle Disease Virus

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Molecular characterization of bovine ephemeral fever virus in Thailand between 2013 and 2017

Veterinary Microbiology ◽

10.1016/j.vetmic.2018.10.013 ◽

2018 ◽

Vol 227 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Thanawat Chaisirirat ◽

Pradit Sangthong ◽

Pipat Arunvipas ◽

Nantawan Petcharat ◽

Nattarat Thangthamniyom ◽

...

Keyword(s):

Molecular Characterization ◽

Fever Virus ◽

Bovine Ephemeral Fever Virus ◽

Bovine Ephemeral Fever

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Molecular Characterization of a New Variant of Toxin-Coregulated Pilus Protein (TcpA) in a Toxigenic Non-O1/Non-O139 Strain ofVibrio cholerae

Infection and Immunity ◽

10.1128/iai.68.2.948-952.2000 ◽

2000 ◽

Vol 68 (2) ◽

pp. 948-952 ◽

Cited By ~ 29

Author(s):

Bisweswar Nandi ◽

Ranjan K. Nandy ◽

Ana C. P. Vicente ◽

Asoke C. Ghose

Keyword(s):

Southern Hybridization ◽

Analysis Data ◽

Nucleotide Sequence Analysis ◽

Predicted Amino Acid Sequence ◽

Ancestral Gene ◽

Hypervariable Regions ◽

New Variant ◽

Toxin Coregulated Pilus ◽

El Tor

ABSTRACT A toxigenic non-O1/non-O139 strain of Vibrio cholerae(10259) was found to contain a new variant of the toxin-coregulated pilus (TCP) protein gene (tcpA) as determined by PCR and Southern hybridization experiments. Nucleotide sequence analysis data of the new tcpA gene in strain 10259 (O53) showed it to be about 74 and 72% identical to those of O1 classical and El Tor biotype strains, respectively. The predicted amino acid sequence of the 10259 TcpA protein shared about 81 and 78% identity with the corresponding sequences of classical and El Tor TcpA strains, respectively. An antiserum raised against the TCP of a classical strain, O395, although it recognized the TcpA protein of strain 10259 in an immunoblotting experiment, exhibited considerably less protection against 10259 challenge compared to that observed against the parent strain. Incidentally, the tcpA sequences of two other toxigenic non-O1/non-O139 strains (V2 and S7, both belonging to the serogroup O37) were determined to be almost identical to that of classicaltcpA. Further, tcpA of another toxigenic non-O1/non-O139 strain V315-1 (O nontypeable) was closely related to that of El Tor tcpA. Analysis of these results with those already available in the literature suggests that there are at least four major variants of the tcpA gene in V. cholerae which probably evolved in parallel from a common ancestral gene. Existence of highly conserved as well as hypervariable regions within the sequence of the TcpA protein would also predict that such evolution is under the control of considerable selection pressure.

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