Fluorometric determination of kynurenic acid in human serum by high performance liquid chromatography coupled with postcolumn photochemical reaction with hydrogen peroxide.

1988 ◽  
Vol 4 (2) ◽  
pp. 195-197 ◽  
Author(s):  
Ken-ichi MAWATARI ◽  
Fumio IINUMA ◽  
Mitsuo WATANABE
Keyword(s):  
Hydrogen Peroxide ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Serum ◽  
Photochemical Reaction ◽  
High Performance ◽  
Kynurenic Acid ◽  
Fluorometric Determination

scholarly journals Simultaneous Determination of Kynurenine and Kynurenic Acid by High-Performance Liquid Chromatography Photoirradiation System Using a Mobile Phase Containing 18-crown-6

2019 ◽  
Vol 12 ◽  
pp. 117864691983455
Author(s):  
Motomasa Atsumi ◽  
Ken-ichi Mawatari ◽  
Akari Morooka ◽  
Makoto Yasuda ◽  
Tomoko Fukuuchi ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phosphate Buffer ◽  
Mobile Phase ◽  
High Performance ◽  
Kynurenic Acid ◽  
Fluorometric Determination ◽  
The Mean

A high-performance liquid chromatography (HPLC) system has been developed for the fluorometric determination of kynurenine (KYN) and kynurenic acid (KYNA) in human serum using a mobile phase containing 18-crown-6. A retention time of KYNA was adjusted with pH of phosphate buffer in 18-crown-6. KYN and KYNA were separated on a CAPCELLPAK C18 (250 × 4.6 mm i.d.). The mobile phase consisted of 35 mmol/L phosphate buffer (pH 8.0)/methanol (85/15, v/v) containing 35 mmol/L hydrogen peroxide and 10 mmol/L 18-crown-6. The retention times of KYN and KYNA were 18and 24 minutes, respectively. The calibration graphs of KYN and KYNA were linear over the range 180 to 2900 and 1 to 84 nmol/L by injecting a 50-μL volume of KYN and KYNA, respectively. Pretreatment of serum was achieved by deproteinization only. The mean recoveries of KYN and KYNA from serum were more than 97%.

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